BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0035.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312388|ref|YP_003776480.1| sugar ABC transporter permease ... 291 2e-80 gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease ... 69 2e-13 gi|300313833|ref|YP_003777925.1| sugar ABC transporter permease ... 65 3e-12 gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC tra... 58 5e-10 gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease ... 57 1e-09 >gi|300312388|ref|YP_003776480.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 291 Score = 291 bits (745), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 141/168 (83%), Positives = 154/168 (91%) Query: 1 MNRTQFSITPWLLLMPAMVLLIAFTHYPAVATIWHSFFSTPKGASPSVFVGMENYALMAD 60 M R S+ PWLLL PAMVLLIAFTHYPA+AT+WHSFFSTPKG PSVFVG+ENY LMAD Sbjct: 1 MKRLPSSVLPWLLLSPAMVLLIAFTHYPALATLWHSFFSTPKGVRPSVFVGLENYRLMAD 60 Query: 61 DPVFWRALFNNLWFALGTIPVSIALAIMMALWVNDKLLGRGLLRLAYFTPTVLPMIAVAN 120 DPVFW+AL NNLWFALGTIP++IALAI+MALWVN+KL GRG+LRLAYFTPTVLPMIAVAN Sbjct: 61 DPVFWQALCNNLWFALGTIPLAIALAILMALWVNEKLAGRGVLRLAYFTPTVLPMIAVAN 120 Query: 121 IWLFFYTPQYGLLEQLTNALGLPSHNWLGNPDTVLGCMMVVAIWKEAG 168 IWLFFYTPQYGLLEQ+T ALGLPSHNWLGNP TVLG +MVVA+WKEAG Sbjct: 121 IWLFFYTPQYGLLEQITGALGLPSHNWLGNPQTVLGALMVVAVWKEAG 168 >gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 311 Score = 68.9 bits (167), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 52/154 (33%), Positives = 82/154 (53%), Gaps = 8/154 (5%) Query: 14 LMPAMVLLIAFTHYPAVATIWHSFFSTPKGASPSVFVGMENYALMADDPVFWRALFNN-L 72 L PA V+ + + YP + TI+ SFFS P FVG+ NY + P F+ AL NN L Sbjct: 42 LAPACVMTLVYVLYPILYTIYLSFFSWDGMTEPR-FVGLANYVELFHAPTFYTALKNNVL 100 Query: 73 WFALGTIPVSIALAIMMALWVNDKLLGRGLLRLAYFTPTVLPMIAVANIWLFFYTPQYGL 132 W + + + LAI AL++N K++G +++ +F P VL + V ++ +FY P +GL Sbjct: 101 WLLMFLLAPPMGLAI--ALYLNQKVIGMRVVKSLFFAPFVLSGVVVGLMYSWFYDPSFGL 158 Query: 133 LEQLTNALGLPSHNWLGNPDTVLGCMMVVAIWKE 166 L L G+P LG+ ++ A+W + Sbjct: 159 LSLLFGQ-GVPV---LGDARYATFGIIFAALWPQ 188 >gi|300313833|ref|YP_003777925.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 65.1 bits (157), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 51/169 (30%), Positives = 85/169 (50%), Gaps = 16/169 (9%) Query: 12 LLLMPAMVLLIAFTHYPAVATIWHSFFSTPKGASPSVFVGMENYALMADDP----VFWRA 67 +LL PA+++ F+ P + T+ F+ T A FVG++NY + DP FW A Sbjct: 11 VLLAPAVLIYALFSALPLLDTLRLGFY-TANDAGVRSFVGLDNYRTILFDPDWSAAFWNA 69 Query: 68 LFNNLWF-----ALGTIPVSIALAIMMALWVNDKLLGRGLLRLAYFTPTVLPMIAVANIW 122 ++NN+ F AL P+ + LA + +L L G R F PT+L ++ + IW Sbjct: 70 MWNNVKFFCIHMALQN-PIGLLLATLFSL---KGLRGARTYRTLIFLPTLLSVVIIGFIW 125 Query: 123 LFFYTPQYGLLEQLTNALGLPS--HNWLGNPDTVLGCMMVVAIWKEAGL 169 +P +G+ E+L +GL WLG T L + ++++W+ G+ Sbjct: 126 QLILSPLWGVGEKLLGTVGLADWFAPWLGQESTALLTLSLISVWQYVGI 174 >gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 57.8 bits (138), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 38/167 (22%), Positives = 81/167 (48%), Gaps = 3/167 (1%) Query: 2 NRTQFSIT--PWLLLMPAMVLLIAFTHYPAVATIWHSFFSTPKGASPSVFVGMENYALMA 59 R +F+ P+LL+ P +++ + F +PAV ++ S S FV +N+ + Sbjct: 3 KRARFTSKWLPYLLVAPQIIITLLFFFWPAVQALYQSMLLQDAFGGYSEFVWFDNFKTLF 62 Query: 60 DDPVFWRALFNNLWFALGTIPVSIALAIMMALWVNDKLLGRGLLRLAYFTPTVLPMIAVA 119 DP + + F+ +AL++++A++ + G + + P + + V Sbjct: 63 GDPTYLESFRTTAIFSALVAFFGLALSLLLAVFADRVRRGASIYKTFLIWPYAVSPVVVG 122 Query: 120 NIWLFFYTPQYGLLEQLTNALGLPSHNWLGNPDTVLGCMMVVAIWKE 166 +W+F +P G+L + + LGLP +++ N + +++ AIWK+ Sbjct: 123 VLWMFLLSPSLGILSHVLSWLGLP-WDYMINGTHAMILIVIAAIWKQ 168 >gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 324 Score = 56.6 bits (135), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 44/145 (30%), Positives = 71/145 (48%), Gaps = 10/145 (6%) Query: 12 LLLMPAMVLLIAFTHYPAVATIWHSFFSTPK----GASPSVFVGMENYALMADDPVFWRA 67 L+ P++++L+A+ P TI+ FS + + FVG+ENYA + DP FW + Sbjct: 42 LMQTPSVLILLAWMIVPLAMTIY---FSVNRYNLMAPDQTGFVGLENYAFLLQDPAFWPS 98 Query: 68 LFNNLWFALGTIPVSIALAIMMALWVNDKLLGRGLLRLAYFTP-TVLPMIAVANIWL-FF 125 + N + +S+ ++A+ + GRG+ RL P V+P +A A IW Sbjct: 99 IGNTALLIGSVLAISVIGGTLLAVLFDQPFRGRGIARLLVIGPFFVMPTVA-ALIWKNMI 157 Query: 126 YTPQYGLLEQLTNALGLPSHNWLGN 150 P YG+L + LGL +WL Sbjct: 158 MHPVYGILAYVMRHLGLEPIDWLAE 182 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.140 0.475 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 619,100 Number of extensions: 26540 Number of successful extensions: 118 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 112 Number of HSP's successfully gapped: 8 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 127 (53.5 bits)