BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0016.p (469 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 717 0.0 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 240 2e-64 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 224 1e-59 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 204 2e-53 gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 180 3e-46 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 717 bits (1851), Expect = 0.0, Method: Compositional matrix adjust. Identities = 379/457 (82%), Positives = 400/457 (87%), Gaps = 1/457 (0%) Query: 5 DGSLDWNALYQAVSGGTGEVAPVAAAPVQAAPAEAESSSGHPKSLPSATPTPKP-AAAPV 63 +G L+W ALYQA+ G APVA A +AAP A S+ + + +P AAAP Sbjct: 174 NGELNWPALYQALVPGAAAAAPVAGAVAEAAPVAAASAPAAAVAAAAPAADGEPKAAAPA 233 Query: 64 QARRATDNQPGNAKEESIRIDAGKLDALLEVAGESVQAANQAAVLLEKLQQFKFEGTAAS 123 Q R + PG AKEESIRIDAGKLDALLEVAGESVQAANQAAVLLEKLQQFKFEGTAA+ Sbjct: 234 QHNRRASDSPGGAKEESIRIDAGKLDALLEVAGESVQAANQAAVLLEKLQQFKFEGTAAA 293 Query: 124 LMSTLAETLGRASRYSSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDLAISGA 183 LMSTLAETLGRASRYSSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDL I+GA Sbjct: 294 LMSTLAETLGRASRYSSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDLVITGA 353 Query: 184 ETEVDRVVVDSLYDPLVHMLRNSLDHGIETDRGAAGKPNKATISLKAWQEASSVMIEIFD 243 ETEVDRVVVDSLYDPLVHMLRNSLDHGIE DR AAGKP KATISL AWQ SSVMIE+FD Sbjct: 354 ETEVDRVVVDSLYDPLVHMLRNSLDHGIEVDRVAAGKPTKATISLNAWQAGSSVMIEVFD 413 Query: 244 DGKGMDPDFLRKKAIGKGLIGEHEAQSHDEALQLVFLPGFSTKEVASSVSGRGVGMDVVK 303 DGKGMDPD LR KA+ KGLI EH+A + DEALQLVFLPGFSTKEVASSVSGRGVGMDVVK Sbjct: 414 DGKGMDPDMLRSKALAKGLINEHQALTADEALQLVFLPGFSTKEVASSVSGRGVGMDVVK 473 Query: 304 TAVEKHRGTIRIDSTLGVGTRFSIRLPIELSIIPTMLVRTAGAALALPMAVVERVVELPA 363 TAVEKHRGTIRI+ST+G GT+FSIRLPIELSIIPTMLVR AGA LALPMAVVERVVELP Sbjct: 474 TAVEKHRGTIRIESTIGKGTKFSIRLPIELSIIPTMLVRCAGAPLALPMAVVERVVELPE 533 Query: 364 EFDEVGGAPVLRDQGRPLPVHSLAGVLGYEPGNERVGIVMAVPHPYILSVGAVEGTADLV 423 FDEVGGAPVLRDQGRPLPV+SLAGVLGYEPGNERVGIVMAVPHPYIL V +VEGTADLV Sbjct: 534 NFDEVGGAPVLRDQGRPLPVNSLAGVLGYEPGNERVGIVMAVPHPYILGVESVEGTADLV 593 Query: 424 IKPLTAIQTNGITGTARSAEGELVLVVGLSFLLEGCR 460 IKPLTAI T GITGTARSAEGELVLVVGLSFLL+GCR Sbjct: 594 IKPLTAIHTAGITGTARSAEGELVLVVGLSFLLDGCR 630 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 240 bits (613), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 152/435 (34%), Positives = 245/435 (56%), Gaps = 20/435 (4%) Query: 40 ESSSGHPKSLPSATPTPKPAAAPVQARRATDNQPGNAKEESIRIDAGKLDALLEVAGESV 99 E++S P +A P A +Q RA D + +++ I+I+ KLD L+++ GE V Sbjct: 294 EAASATPSDALAAAVEPMAAEGVLQRPRALDERRAQ-EQKFIKIEVSKLDQLIDLVGELV 352 Query: 100 QAANQAAVLLEKLQQFKFEGTAASLMSTLAETLGRASRYSSELQRATLSTRMQPVGRLFQ 159 A A ++ + + +FE A + TL E +++ A L+ RM + +FQ Sbjct: 353 IAGASARLIARRRKDSQFE-EATQAIDTLME----------QIRDAALTLRMVQINEVFQ 401 Query: 160 KFPRLVRELAKDLGKDVDLAISGAETEVDRVVVDSLYDPLVHMLRNSLDHGIE--TDRGA 217 +FPR+VR++A+DLGKD+DL ++G++TE+D+ +V+ L DPL+H++RN++DHGIE +R A Sbjct: 402 RFPRIVRDIARDLGKDIDLVMTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAERLA 461 Query: 218 AGKPNKATISLKAWQEASSVMIEIFDDGKGMDPDFLRKKAIGKGLIGEHEAQSHDEALQL 277 AGKP KA++ L A E+ SV+IE+ DDG+GMD + + KA+ +GL S E + Sbjct: 462 AGKPAKASLQLNAIHESGSVVIEVMDDGRGMDREAILAKAVAQGLASPDAELSDAEIYRF 521 Query: 278 VFLPGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIRIDSTLGVGTRFSIRLPIELSIIP 337 VF PGFST + +SGRGVGMDVV+ +E G + ID+ G GT +RLP+ L+II Sbjct: 522 VFEPGFSTARQVTELSGRGVGMDVVRRNIEALHGEVSIDTGRGRGTVVRLRLPLTLAIIS 581 Query: 338 TMLVRTAGAALALPMAVVERVVELPAEFDEVGGAPV-LRDQGRPL-PVHSLAGVLGYEPG 395 V A +P+ +V +++P + + G V +RD+ P P+ L + E G Sbjct: 582 GFQVVVGNAVFVIPLDMVVECIDMPPQ--QPGSHIVNVRDEPLPFVPLRELFDLPARERG 639 Query: 396 NERVGIVMAVPHPYILSVGAVEGTADLVIKPLTAI--QTNGITGTARSAEGELVLVVGLS 453 + IV P L V + G VIKPL + + G++G+ +G + L++ ++ Sbjct: 640 RNSLVIVQYGPLRAGLLVDGLLGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILDIA 699 Query: 454 FLLEGCRSAAKAVAQ 468 L+ + + AQ Sbjct: 700 HLVHHTQQQEQGSAQ 714 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 224 bits (571), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 123/302 (40%), Positives = 188/302 (62%), Gaps = 12/302 (3%) Query: 66 RRATDNQPGNAKEESIRIDAGKLDALLEVAGESVQAANQAAVLLEKLQ-QFKFEGTAASL 124 RRA ++ GN +S++++ K+D L+ + GE V + N L ++ + QF A + Sbjct: 439 RRAEESLSGN---KSVKVEQVKIDRLMNLIGEMVVSKNALPYLAQRAEDQFGVRELAREI 495 Query: 125 MSTLAETLGRASRYSSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDLAISGAE 184 + A +R + E+Q A + RM PV +FQ+FPRLVR++++ LGK+V L + G E Sbjct: 496 KAQYAVI----NRIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEE 551 Query: 185 TEVDRVVVDSLYDPLVHMLRNSLDHGIE--TDRGAAGKPNKATISLKAWQEASSVMIEIF 242 TE D+ ++++L DPL+H++RNSLDHG+E R A+GKP ++++ A E V+I I Sbjct: 552 TEADKNIIEALADPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIE 611 Query: 243 DDGKGMDPDFLRKKAIGKGLIGEHEAQ--SHDEALQLVFLPGFSTKEVASSVSGRGVGMD 300 DDGKG+DP +++KA KGLI E + + S +A+ LVF GFST EV S +SGRGVGMD Sbjct: 612 DDGKGIDPALIKRKAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMD 671 Query: 301 VVKTAVEKHRGTIRIDSTLGVGTRFSIRLPIELSIIPTMLVRTAGAALALPMAVVERVVE 360 VV+ AVEK G++ + S G GTR I LP+ +++ M++ + G +PM V V Sbjct: 672 VVRNAVEKVNGSLALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETVR 731 Query: 361 LP 362 +P Sbjct: 732 VP 733 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 204 bits (518), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 156/453 (34%), Positives = 234/453 (51%), Gaps = 23/453 (5%) Query: 20 GTGEVAPVAAAPVQAAPAEAES-SSGHPKSLPSATPTPKPAAAPVQARRATDNQPGNAKE 78 G G AP + A +AE+ S HP P A T AA A+ A G+ + Sbjct: 247 GYGFFAPFVPLDISAKKPDAETLSQPHPDDAPLAV-TEHAAAEKKAAKAAAAAAAGH-ES 304 Query: 79 ESIRIDAGKLDALLEVAGESVQAANQAAVLLEKLQQFKFEGTAASLMSTLAETLGRASRY 138 SIR+ K+D L+ + GE V + KL E L ++ + SR Sbjct: 305 SSIRVSVEKVDQLINLVGELVITQAMIEQRVGKLDPMTHE--------HLLNSVSQLSRN 356 Query: 139 SSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDLAISGAETEVDRVVVDSLYDP 198 + +LQ + +S RM P+ +F +FPR+VR+LA LGK V+ GA TE+D+ +++ + DP Sbjct: 357 TRDLQESVMSIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDKGLIERIVDP 416 Query: 199 LVHMLRNSLDHGIE--TDRGAAGKPNKATISLKAWQEASSVMIEIFDDGKGMDPDFLRKK 256 L H++RNS+DHGIE R A+GK T+SL A + +++IE+ DDG G++ + + K Sbjct: 417 LTHLVRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAK 476 Query: 257 AIGKGLIGEHEAQSHDEALQLVFLPGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIRID 316 A +G+ + S E QL+F PGFST E + VSGRGVGMDVVK + G + I Sbjct: 477 AKQQGM-PVTDNLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIR 535 Query: 317 STLGVGTRFSIRLPIELSIIPTMLVRTAGAALALPMA-VVERVVELPAEFDEVGG-APVL 374 S+ G GT SI LP+ L+I+ M +R LP+ VVE + L + E+ G VL Sbjct: 536 SSKGFGTTISISLPLTLAILDGMSIRIGEEVYILPLGYVVESLQPLAQDIKEISGQGQVL 595 Query: 375 RDQGRPLPVHSLAGVLGYEPG--NERVGIVMAVP---HPYILSVGAVEGTADLVIKPLTA 429 R +G LP+ L + EP + G+V+ + L V + G +V+K L + Sbjct: 596 RVRGEYLPLIPLYQIFNIEPSFTDPSQGLVVILESDGKKAALFVDDLIGQQQIVVKNLES 655 Query: 430 I--QTNGITGTARSAEGELVLVVGLSFLLEGCR 460 + GI+G +G + L+V ++ LL R Sbjct: 656 NYRKVAGISGATILGDGGVALIVDVAALLRSSR 688 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 180 bits (456), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 139/397 (35%), Positives = 206/397 (51%), Gaps = 49/397 (12%) Query: 80 SIRIDAGKLDALLEVAGESVQAANQ-----AAVL------------LEKLQQF------- 115 ++R+ A LD LL + GES+ + Q A++L L+ LQQ Sbjct: 197 AVRVSADSLDRLLGLTGESLVESRQLKPFSASMLRMKRTQREAMQALDLLQQKLATTQLD 256 Query: 116 KFEGTAASLMSTLAET----LG-----------RASRYSSELQRATLSTRMQPVGRLFQK 160 +F TA + + TL + LG R+ S L L+ RM+P Sbjct: 257 EFAQTAFAELRTLMQQNQQLLGEQLSALDAFDRRSVNLSQRLYDEALACRMRPFADGTAG 316 Query: 161 FPRLVRELAKDLGKDVDLAISGAETEVDRVVVDSLYDPLVHMLRNSLDHGIET--DRGAA 218 R+VR++ LGK V L I G T++DR +++ L P+ H+LRN++DHGIE+ R AA Sbjct: 317 LARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLLRNAVDHGIESSAQRRAA 376 Query: 219 GKPNKATISLKAWQEASSVMIEIFDDGKGMDPDFLRKKAIGKGLIGEHEAQ--SHDEALQ 276 GKP + I L+A A ++IE+ DDG G+D + +R + +GL + A+ S E L+ Sbjct: 377 GKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRSAVVARGLSNQETAERLSEAELLE 436 Query: 277 LVFLPGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIRIDSTLGVGTRFSIRLPIELSII 336 + LP FS ++ + +SGRGVG+DVV +++ RGTIRI + G G+RF ++LP+ LS+I Sbjct: 437 FLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQAGQGSRFLMQLPLTLSVI 496 Query: 337 PTMLVRTAGAALALPMAVVERVVELPAE-FDEVGGAPVLRDQGRPLPVHSLAGVLGYEPG 395 ++LV AG A P+A V R + LPAE + G GR + + S VL Sbjct: 497 RSLLVDIAGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGGRQVGLVSAHQVLQKGQW 556 Query: 396 NERVGIVMAV-----PHPYILSVGAVEGTADLVIKPL 427 R G V V H Y L+V A G LV++PL Sbjct: 557 RSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPL 593 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.132 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,339,476 Number of extensions: 56060 Number of successful extensions: 218 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 8 Length of query: 469 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 380 Effective length of database: 1,198,455 Effective search space: 455412900 Effective search space used: 455412900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)