BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0015.p
         (442 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herba...   553   e-158
gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba...    70   4e-13

>gi|300310046|ref|YP_003774138.1| chloride-channel protein
           [Herbaspirillum seropedicae SmR1]
          Length = 441

 Score =  553 bits (1424), Expect = e-158,   Method: Compositional matrix adjust.
 Identities = 289/419 (68%), Positives = 344/419 (82%), Gaps = 6/419 (1%)

Query: 17  RRLWLQYGILWLGAVLVGLVAVMYAWLIDFGFEMFSAMQRDHVWLPLVLTPAMSALCVWL 76
           RRLW++YGILW GAV+VGLVAV+YA  I+ GF +F ++Q    WLPL+LTPA+ A CVWL
Sbjct: 26  RRLWVEYGILWSGAVIVGLVAVLYAQAIEIGFGVFRSIQHRSPWLPLLLTPAVCAACVWL 85

Query: 77  TRRFFPGAEGSGIPQVIASLAEENDRGTPRPSRAPALLTLKILGGKIGISFLGILGGFTI 136
           TRR+FPG+EGSGIPQ IA+L  +         R   LL+L++L GKI +S + I GG TI
Sbjct: 86  TRRYFPGSEGSGIPQAIATLHGDTAE------RGSTLLSLRVLLGKIVLSVVAIAGGMTI 139

Query: 137 GREGPTVHVGAALMYNMRRFYPRPSAILERRLILAGAAAGLSAAFNTPLAGIVFAIEELS 196
           GREGPTV +GAA+MYN+RR YP     +ERRLILAGAAAGL+AAFNTPLAGIVFAIEELS
Sbjct: 140 GREGPTVQIGAAIMYNLRRLYPAGYEHMERRLILAGAAAGLAAAFNTPLAGIVFAIEELS 199

Query: 197 RSFEQRASGIVITTIIFAGIVALGLNGNYTYFGTINAGNDSARELIVAVVVSGILMGVAG 256
           RSFEQRASG+VITTIIFAG+VAL +NGNYTYFG+I    +++  LI+AV+V+ +LMGVAG
Sbjct: 200 RSFEQRASGVVITTIIFAGLVALSINGNYTYFGSIAFPGETSGRLILAVIVTAVLMGVAG 259

Query: 257 GIFCWLLLNTHKWIPAPLRTLRAERPVVFGALCGLVVAIVGVCAGGVTFGSGYAEAKGLL 316
           G+FCWL+LN  +WIP  L TLR ERPV F A+CG +VA++GV AGG TFGSGY +A GLL
Sbjct: 260 GLFCWLILNPTRWIPPALVTLRTERPVAFAAVCGFIVAVIGVAAGGATFGSGYEQAHGLL 319

Query: 317 SGQTQLSAWYPVAKMASMVGSYLPGIPGGIFAPSLSIGAGFGNLLHMVFEHTSLSMLIAL 376
           +    LS +YP  K ASM+GSYLPG+PGGIFAPSLSIGAGFGNLLH++F  TSL ML+AL
Sbjct: 320 NDGQGLSVFYPFLKFASMIGSYLPGLPGGIFAPSLSIGAGFGNLLHLLFGGTSLPMLVAL 379

Query: 377 AMVGYLAAVTQSPITAFVIVMEMINGHALVISLMATALLASRVSRFFAPPLYEALSERY 435
           AMVGYLAAVTQSPITAFVIVMEM++GHALVISLMATAL+AS VS+ FAPPLYE L+ERY
Sbjct: 380 AMVGYLAAVTQSPITAFVIVMEMVDGHALVISLMATALIASAVSKVFAPPLYETLAERY 438


>gi|300311435|ref|YP_003775527.1| chloride channel protein
           [Herbaspirillum seropedicae SmR1]
          Length = 555

 Score = 70.1 bits (170), Expect = 4e-13,   Method: Compositional matrix adjust.
 Identities = 89/307 (28%), Positives = 138/307 (44%), Gaps = 36/307 (11%)

Query: 133 GFTIGREGPTVHVGAALMYNMRRFYPRPSAILERRLILAGAAAGLSAAFNTPLAGIVFAI 192
           G +IGREGP V + A     + +F   PS+ L R L+  GAAAG+++A+N P+AG  F  
Sbjct: 108 GGSIGREGPMVQLSALCASLVGKFSHFPSSRL-RLLVACGAAAGITSAYNAPIAGAFFIT 166

Query: 193 EELSRSFEQRASGIVITTIIFAGIVALGLNG-----NYTYFGTINAGNDSARELIVAVVV 247
           E +  S    + G V+   + A I    L G        YF  I          ++  +V
Sbjct: 167 EIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWE------VMLFLV 220

Query: 248 SGILMGVAGGIFCWLL-LNTHKW----IPAPLRTLRAERPVVFGALCGLVVAIVGVCAGG 302
            G+L GV    F  +L L  H +    +P P+R            +  + V +  V    
Sbjct: 221 LGVLAGVLAPQFLRVLELGKHGFGKLRLPLPVRLGVGGL-----LVGLISVQVPEV---- 271

Query: 303 VTFGSGYAEAKGLLSGQTQLSAWYPVAKMASMVGSYL--PGIPGGIFAPSLSIGAGFGNL 360
             +G+GY+    LL       A   V  +  +  S     G  GGIF P+L +GA  G L
Sbjct: 272 --WGNGYSLVNSLLHTSWVWQAVLMVLVVKVLATSITVGSGAVGGIFTPTLFVGAAVGYL 329

Query: 361 LHMVFEH---TSLSMLIALAMVG---YLAAVTQSPITAFVIVMEMINGHALVISLMATAL 414
                +      +S   A AMVG   +LAA + +P+ A +++ EM   + +V+ LM + +
Sbjct: 330 FGDAAQALLPVHISQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCV 389

Query: 415 LASRVSR 421
           +A  V+R
Sbjct: 390 VAYVVAR 396


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.141    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,214,926
Number of extensions: 45452
Number of successful extensions: 269
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 263
Number of HSP's successfully gapped: 4
Length of query: 442
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 354
Effective length of database: 1,203,190
Effective search space: 425929260
Effective search space used: 425929260
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 132 (55.5 bits)