BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0006.p (441 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312403|ref|YP_003776495.1| sulfate adenylyltransferase sub... 781 0.0 gi|300309454|ref|YP_003773546.1| GTPase translation elongation f... 76 5e-15 gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein... 76 5e-15 >gi|300312403|ref|YP_003776495.1| sulfate adenylyltransferase subunit 1 protein [Herbaspirillum seropedicae SmR1] Length = 443 Score = 781 bits (2016), Expect = 0.0, Method: Compositional matrix adjust. Identities = 377/427 (88%), Positives = 405/427 (94%) Query: 14 SHERGLLRFITAGSVDDGKSTLIGRLLFDSKGIFADQLDAMSRAKHKRTVGDTIDLSLLT 73 SHERGLLRFITAGSVDDGKSTLIGRLLFDSKGIFADQLDA+SRAKHKRTVGDT+DLSLLT Sbjct: 17 SHERGLLRFITAGSVDDGKSTLIGRLLFDSKGIFADQLDAISRAKHKRTVGDTVDLSLLT 76 Query: 74 DGLEAEREQGITIDVAYRYFATPKRKFIIADTPGHEQYTRNMVTGASTADAVIILIDVSK 133 DGLEAEREQGITIDVAYRYFATPKRKFIIADTPGHEQYTRNMVTGASTADAVIIL+DVSK Sbjct: 77 DGLEAEREQGITIDVAYRYFATPKRKFIIADTPGHEQYTRNMVTGASTADAVIILVDVSK 136 Query: 134 VKLGDDGSVELLTQTKRHSTIAHLLQIQHIVVAVNKMDLVDYDQTVYDRIVGAYQKFAAQ 193 VKLG+DGSVELLTQTKRHSTIAHLLQIQH++VAVNKMDLVDYDQTVYDRIVGAYQ+FA Q Sbjct: 137 VKLGEDGSVELLTQTKRHSTIAHLLQIQHVIVAVNKMDLVDYDQTVYDRIVGAYQQFAQQ 196 Query: 194 LGLKDVRPIPLSALAGDNVVTASEKLSWYKGPTLIALLESLSVYEDAHEEPFRFPVQLVA 253 LGL+DV IPLSALAGDNVV AS ++ WY+GPTLI LLESLSVYEDAHEE FRFPVQLVA Sbjct: 197 LGLQDVHAIPLSALAGDNVVEASARMPWYQGPTLITLLESLSVYEDAHEEAFRFPVQLVA 256 Query: 254 RHNGHEANDFRGYMGRIEAGKVSKGDKLLVQPSGQSATVKDILTLDGSLQSAAAGQSVTI 313 RHNGHEANDFRGYMGRIEAGKV++GDKL+V PSGQSATVKDI TLDGSL+SA+AGQSVTI Sbjct: 257 RHNGHEANDFRGYMGRIEAGKVARGDKLVVLPSGQSATVKDIQTLDGSLESASAGQSVTI 316 Query: 314 LLDEYLDISRGDMLASAEQAPTQLKTIDADLCWLSEDALDPRRKYWLKHTTKQVAARVTK 373 LLDEYLDISRGD+LASA Q T LKT++AD+CWLSE+ LD RRKYWLKHTTKQVAARV K Sbjct: 317 LLDEYLDISRGDLLASAAQPSTILKTVNADVCWLSEEPLDLRRKYWLKHTTKQVAARVAK 376 Query: 374 IDTLLDINTQQRHPAESLKLNDIARVSINVQQAIAVDGYDAIRATGAFILIDEVTHQTVA 433 +DTLLDINTQ+R PAE+LKLNDIAR+S+NVQQ IA D Y AIRATGAFILIDEV+HQTVA Sbjct: 377 VDTLLDINTQERRPAETLKLNDIARISVNVQQPIAADAYQAIRATGAFILIDEVSHQTVA 436 Query: 434 AGMIRLD 440 AGMIR+D Sbjct: 437 AGMIRID 443 >gi|300309454|ref|YP_003773546.1| GTPase translation elongation factor TU [Herbaspirillum seropedicae SmR1] Length = 396 Score = 76.3 bits (186), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 98/411 (23%), Positives = 170/411 (41%), Gaps = 79/411 (19%) Query: 24 TAGSVDDGKSTLIGRLL------FDSKGIFADQLDAMSRAKHKRTVGDTIDLSLLTDGLE 77 T G VD GK+TL + F + DQ+DA Sbjct: 17 TIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPE--------------------- 55 Query: 78 AEREQGITIDVAYRYFATPKRKFIIADTPGHEQYTRNMVTGASTADAVIILIDVSKVKLG 137 E+ +GITI+ A+ + T R + D PGH Y +NM+TGA+ D I++ + Sbjct: 56 -EKARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA----- 109 Query: 138 DDGSVELLTQTKRHSTIAHLLQIQHIVVAVNKMDLVDYDQTVYDRIVGAYQKFAAQLGLK 197 DG + QT+ H ++ + + +I+V +NK D+VD D + + + ++ ++ Sbjct: 110 -DGP---MPQTREHILLSRQVGVPYIIVFLNKADMVD-DAELLELVEMEVRELLSKY--- 161 Query: 198 DVRPIPLSALAGDN--VVTASEKLSWY--KGP----TLIALLESLSVY----EDAHEEPF 245 GD+ +V S KL+ GP ++AL E+L Y E A + F Sbjct: 162 --------EFPGDDLPIVKGSAKLALEGDTGPLGEQAIMALAEALDTYIPTPERAVDGTF 213 Query: 246 RFPVQLVARHNGHEANDFRGYM--GRIEAGKVSKGDKLLVQ--PSGQSATVKDILTLDGS 301 PV+ V +G RG + GR+E G + G+++ + Q T + Sbjct: 214 LMPVEDVFSISG------RGTVVTGRVERGIIKVGEEIEIVGIADTQKTTCTGVEMFRKL 267 Query: 302 LQSAAAGQSVTILL--DEYLDISRGDMLASAEQAPTQLKTIDADLCWLSEDALDPRRKYW 359 L AG +V +LL + D+ RG +LA K ++ LS+D R + Sbjct: 268 LDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGSIKPH-KHFTGEIYVLSKDE-GGRHTPF 325 Query: 360 LKHTTKQVAARVTKIDTLLDINTQQRHPAESLKLNDIARVSINVQQAIAVD 410 + Q R T + +++ + E + D +++ + IA++ Sbjct: 326 FNNYRPQFYFRTTDVTGAIELPKDK----EMVMPGDNVSITVQLINPIAME 372 >gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 76.3 bits (186), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 98/411 (23%), Positives = 170/411 (41%), Gaps = 79/411 (19%) Query: 24 TAGSVDDGKSTLIGRLL------FDSKGIFADQLDAMSRAKHKRTVGDTIDLSLLTDGLE 77 T G VD GK+TL + F + DQ+DA Sbjct: 17 TIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPE--------------------- 55 Query: 78 AEREQGITIDVAYRYFATPKRKFIIADTPGHEQYTRNMVTGASTADAVIILIDVSKVKLG 137 E+ +GITI+ A+ + T R + D PGH Y +NM+TGA+ D I++ + Sbjct: 56 -EKARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA----- 109 Query: 138 DDGSVELLTQTKRHSTIAHLLQIQHIVVAVNKMDLVDYDQTVYDRIVGAYQKFAAQLGLK 197 DG + QT+ H ++ + + +I+V +NK D+VD D + + + ++ ++ Sbjct: 110 -DGP---MPQTREHILLSRQVGVPYIIVFLNKADMVD-DAELLELVEMEVRELLSKY--- 161 Query: 198 DVRPIPLSALAGDN--VVTASEKLSWY--KGP----TLIALLESLSVY----EDAHEEPF 245 GD+ +V S KL+ GP ++AL E+L Y E A + F Sbjct: 162 --------EFPGDDLPIVKGSAKLALEGDTGPLGEQAIMALAEALDTYIPTPERAVDGTF 213 Query: 246 RFPVQLVARHNGHEANDFRGYM--GRIEAGKVSKGDKLLVQ--PSGQSATVKDILTLDGS 301 PV+ V +G RG + GR+E G + G+++ + Q T + Sbjct: 214 LMPVEDVFSISG------RGTVVTGRVERGIIKVGEEIEIVGIADTQKTTCTGVEMFRKL 267 Query: 302 LQSAAAGQSVTILL--DEYLDISRGDMLASAEQAPTQLKTIDADLCWLSEDALDPRRKYW 359 L AG +V +LL + D+ RG +LA K ++ LS+D R + Sbjct: 268 LDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGSIKPH-KHFTGEIYVLSKDE-GGRHTPF 325 Query: 360 LKHTTKQVAARVTKIDTLLDINTQQRHPAESLKLNDIARVSINVQQAIAVD 410 + Q R T + +++ + E + D +++ + IA++ Sbjct: 326 FNNYRPQFYFRTTDVTGAIELPKDK----EMVMPGDNVSITVQLINPIAME 372 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,304,246 Number of extensions: 52991 Number of successful extensions: 169 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 3 Length of query: 441 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 353 Effective length of database: 1,203,190 Effective search space: 424726070 Effective search space used: 424726070 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)