BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0087.p (898 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 compo... 1798 0.0 gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 compo... 963 0.0 >gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 898 Score = 1798 bits (4657), Expect = 0.0, Method: Compositional matrix adjust. Identities = 860/897 (95%), Positives = 883/897 (98%) Query: 1 MSAQIDQVLAQAANDPDVIETQEWLDALEAVIEAEGPERAHYLMERMVDLARRRGAHIPF 60 MSAQIDQVLAQAANDPDV+ETQEWLDALEAVIE EGP+RAHYLMERMVDLARRRGAHIPF Sbjct: 1 MSAQIDQVLAQAANDPDVMETQEWLDALEAVIEKEGPDRAHYLMERMVDLARRRGAHIPF 60 Query: 61 SSNTAYVNTIPADQGEHCPGNLEIEERLRSWMRWNAMAMVVKANRVDGDLGGHLSSFASL 120 SSNTAYVNTIPADQGEHCPGNLE EERLRSWMRWNAMAMVVKANRVDGDLGGHLSSFASL Sbjct: 61 SSNTAYVNTIPADQGEHCPGNLEYEERLRSWMRWNAMAMVVKANRVDGDLGGHLSSFASL 120 Query: 121 ANMLGIGFNHFWHAPTEDHGGDLLYIQGHSSPGIYARAFLEGRLTEDQLIHFRREADGHG 180 ANMLGIGFNHFWHAPTEDHGGDLLYIQGHSSPGIYARAFLEGRLTEDQLIHFRREADGHG Sbjct: 121 ANMLGIGFNHFWHAPTEDHGGDLLYIQGHSSPGIYARAFLEGRLTEDQLIHFRREADGHG 180 Query: 181 LSSYPHPKLMPEFWQFPTVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEM 240 LSSYPHPKLMPEFWQFPTVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEM Sbjct: 181 LSSYPHPKLMPEFWQFPTVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEM 240 Query: 241 DEPESMGAIGMAGREKLNNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVI 300 DEPESMGAIGMAGREKLNNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVI Sbjct: 241 DEPESMGAIGMAGREKLNNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVI 300 Query: 301 WGSGWDELLAKDKEGILQKVMMETVDGEYQNYKAKDGAFVRKHFFGKHPKLLEMVSKMSD 360 WGSGWDELLAKDK+GILQKVMMETVDGEYQNYKAKDGA+VRKHFFGKHPKLLEMVSKMSD Sbjct: 301 WGSGWDELLAKDKDGILQKVMMETVDGEYQNYKAKDGAYVRKHFFGKHPKLLEMVSKMSD 360 Query: 361 DDIWRLTRGGHDPHKIYAAFKVAQDSKDQPTVILAKSIKGYGFGKSGEARNTAHNTKKLD 420 DDIWRLTRGGHDPHKIYAAFKVAQ+SKDQPTVILAKSIKGYGFGK+GEARNTAHNTKKLD Sbjct: 361 DDIWRLTRGGHDPHKIYAAFKVAQESKDQPTVILAKSIKGYGFGKAGEARNTAHNTKKLD 420 Query: 421 DEAIKAMRDRFQLPISDADLPSIPFFKPSEDTPEMQYLHERRKALGGYLPQRRPQADEKL 480 DEAI+AMRDRFQLPISDADLP+IPFFKPS+D PEMQYLHERRKALGGYLPQRR QADEKL Sbjct: 421 DEAIRAMRDRFQLPISDADLPNIPFFKPSDDAPEMQYLHERRKALGGYLPQRRVQADEKL 480 Query: 481 VVPELSAFQVMLEPTAEGREISTTAAYSRVLTALLRDSSLGQRVVPIMVDESRTFGMEGL 540 VPELSAFQ MLEPTAEGREISTTAAYSRVLTALLRD +LG RVVPIMVDESRTFGMEGL Sbjct: 481 PVPELSAFQAMLEPTAEGREISTTAAYSRVLTALLRDPNLGPRVVPIMVDESRTFGMEGL 540 Query: 541 FRQIGIFSQVGQLYEPVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRI 600 FRQIGIFSQVGQLYEPVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNR+ Sbjct: 541 FRQIGIFSQVGQLYEPVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRV 600 Query: 601 MIPFYTYYSMFGLQRIGDLAWAAGDMRARGFLIGGTAGRTTLNGEGLQHEDGHSHVFASA 660 MIPFYTYYSMFGLQRIGDLAWAAGDMRARGFLIGGTAGRTTLNGEGLQHEDGHSHVFASA Sbjct: 601 MIPFYTYYSMFGLQRIGDLAWAAGDMRARGFLIGGTAGRTTLNGEGLQHEDGHSHVFASA 660 Query: 661 IPNCVPYDPTFAHEVAVIIHDGLRRMVGNQEDVFYYITVMNENYAHPGIKPGQEEGIIKG 720 IPNC+PYDPTFAHEVAVIIHDGLRRMV NQEDVFYYITVMNENY HPGIKPGQEEGIIKG Sbjct: 661 IPNCIPYDPTFAHEVAVIIHDGLRRMVANQEDVFYYITVMNENYGHPGIKPGQEEGIIKG 720 Query: 721 LYLLNEGGKKNKLRVQLLGSGTILREVIAAADLLRDDWKVDADVWSAPSFTLLARDGQDS 780 LYLLNEGGK+NKLRVQLLGSGTILREVIAAADLLRDDWKVDADVWSAPSFTLLARDGQD Sbjct: 721 LYLLNEGGKQNKLRVQLLGSGTILREVIAAADLLRDDWKVDADVWSAPSFTLLARDGQDV 780 Query: 781 ERWNMLHPAEAARKSHFEQSMEGTEGPIVVSTDYMRTYAEQVRAFVPKGRTYKVLGTDGY 840 ERWNMLHPAEA +KS+FEQS+EG+EGPIVVSTDYMRTYAEQVRAFVPKGR+YKVLGTDGY Sbjct: 781 ERWNMLHPAEAPKKSYFEQSLEGSEGPIVVSTDYMRTYAEQVRAFVPKGRSYKVLGTDGY 840 Query: 841 GRSDTRAKLREFFEVDRHYVTVAALKSLADEGKIKPSVVAEAIAKYGINPEKPNPVT 897 GRSDTRAKLREFFEV+R++VTVAALKSLADEGKIKP VVA+AIAKYGI+P KPNPVT Sbjct: 841 GRSDTRAKLREFFEVNRYFVTVAALKSLADEGKIKPEVVAQAIAKYGIDPNKPNPVT 897 >gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 963 bits (2489), Expect = 0.0, Method: Compositional matrix adjust. Identities = 477/887 (53%), Positives = 631/887 (71%), Gaps = 20/887 (2%) Query: 15 DPDVIETQEWLDALEAVIEAEGPERAHYLMERMVDLARRRGAHIPFSSNTAYVNTIPADQ 74 D D ETQEWL AL++V+ GPERA +L+ R+ A++ G + + NT YVNTI +Q Sbjct: 14 DIDSQETQEWLQALQSVLAEAGPERARFLLSRLSAAAQQWGVNWRDARNTPYVNTIRPEQ 73 Query: 75 GEHCPGNLE---IEERLRSWMRWNAMAMVVKANRVDGDLGGHLSSFASLANMLGIGFNHF 131 PG + IEER+ S MRWNA+AMVV+ANR G+LGGH++SFAS A++ +GFNHF Sbjct: 74 EPPFPGGSDAQAIEERIASIMRWNALAMVVRANRAYGELGGHIASFASAADLFEVGFNHF 133 Query: 132 WHAPTEDHGGDLLYIQGHSSPGIYARAFLEGRLTEDQLIHFRREADG-----HGLSSYPH 186 + A E GD++Y Q HS+PGIYARAFLEG L+ED L ++RRE + GLSSYPH Sbjct: 134 FRARDEQFAGDVVYFQPHSAPGIYARAFLEGALSEDDLAYYRREIEAKRVGRQGLSSYPH 193 Query: 187 PKLMPEFWQFPTVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEMDEPESM 246 P LMP+FWQFPT SMG+GP+ AIYQARFL+Y+ RG+ + RKVW GDGEMDEPES+ Sbjct: 194 PWLMPQFWQFPTGSMGIGPINAIYQARFLRYMEHRGLLQDQGRKVWGVFGDGEMDEPESL 253 Query: 247 GAIGMAGREKLNNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVIWGSGWD 306 A+ +A REKL+NL+ VVNCNLQRLDGPVRGNG I+ ELE+ F GAGWNV+K++WGS WD Sbjct: 254 AALSLASREKLDNLIFVVNCNLQRLDGPVRGNGHIVDELETLFAGAGWNVIKLLWGSDWD 313 Query: 307 ELLAKDKEGILQKVMMETVDGEYQNYKAKDGAFVRKHFFGKHPKLLEMVSKMSDDDIWRL 366 L A+DK+G L + TVDG+ Q + A DGAF R+HFFG+ P + + ++D++I RL Sbjct: 314 GLFARDKDGELVDALNRTVDGQLQTFAANDGAFNRQHFFGQTPGTRAIGATLTDEEINRL 373 Query: 367 TRGGHDPHKIYAAFKVAQDSKDQPTVILAKSIKGYGFGKSGEARNTAHNTKKLDDEAIKA 426 RGGHD KIY+A++ A + QPTVILA++ KGYG G +GE + T H KKLD+E++ Sbjct: 374 RRGGHDMKKIYSAYQAAARHRGQPTVILAQTKKGYGMGAAGEGKMTTHQQKKLDEESLLQ 433 Query: 427 MRDRFQLPISDADLPSIPFFKPSEDTPEMQYLHERRKALGGYLPQRRPQADEKLVVPELS 486 RDRF+LP+SD ++ F+KP++D+PEM++LH RR AL GYLP+R+ + V P + Sbjct: 434 FRDRFKLPLSDEQCRALAFYKPADDSPEMKHLHARRAALSGYLPRRQAGQARRAVPPLAA 493 Query: 487 AFQVMLEPTAEGREISTTAAYSRVLTALLRDSSLGQRVVPIMVDESRTFGMEGLFRQIGI 546 + LE A+G+E+S+T A R L LL+D GQ VVPI+ DE+RTFGM LFRQ+GI Sbjct: 494 HSKFALE--ADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADEARTFGMANLFRQVGI 551 Query: 547 FSQVGQLYEPVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRIMIPFYT 606 +S GQLYEP D ++YYRE K GQIL+EGI EAG +SSW AAAT+YS + M+PFY Sbjct: 552 YSSQGQLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAAATAYSVHGTPMLPFYI 611 Query: 607 YYSMFGLQRIGDLAWAAGDMRARGFLIGGTAGRTTLNGEGLQHEDGHSHVFASAIPNCVP 666 YYSMFG QRIGDL WAA D RARGFLIG T+GRTTL GEGLQH+DG+S V A++IPNCV Sbjct: 612 YYSMFGFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDGNSLVTAASIPNCVS 671 Query: 667 YDPTFAHEVAVIIHDGLRRMVGNQEDVFYYITVMNENYAHPGIKPGQEEGIIKGLYLLNE 726 YDP +A+E+AVI+ +G+RRM+ +DVFYYITV NEN A P + G ++GI++GLY L Sbjct: 672 YDPAYAYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLPEGVQDGILRGLYCL-- 729 Query: 727 GGKKNKLRVQLLGSGTILREVIAAADLLRDDWKVDADVWSAPSFTLLARDGQDSERWNML 786 +K + + +LLGSG IL+E +AAA LL+ W+++A+VWS S+T LARDG ++R L Sbjct: 730 -ARKPQAQARLLGSGPILKEAVAAAALLQQHWQIEAEVWSVTSYTELARDGVAAQRAQRL 788 Query: 787 HPAEAARKSHFEQSMEGTEGPIVVSTDYMRTYAEQVRAFVPKGRTYKVLGTDGYGRSDTR 846 A +A + Q ++G+ P++ +DY+RT E +RAFVP Y LGTDG+GRSDTR Sbjct: 789 --AGSAALPYVTQQLQGSAVPVIAVSDYIRTLPESIRAFVPA--PYVTLGTDGFGRSDTR 844 Query: 847 AKLREFFEVDRHYVTVAALKSLADEGKIKPSVVAEAIAKYGINPEKP 893 LR+FFE+D ++ AL + + + + + EA GI+ KP Sbjct: 845 VNLRDFFEIDARWIAYTALCEVWGDDRAR---LQEAARTLGIDTAKP 888 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,017,468 Number of extensions: 135875 Number of successful extensions: 354 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 347 Number of HSP's successfully gapped: 2 Length of query: 898 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 804 Effective length of database: 1,174,780 Effective search space: 944523120 Effective search space used: 944523120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)