BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0026.0085.p
         (321 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransfer...   575   e-165
gi|300312272|ref|YP_003776364.1| dihydrolipoamide succinyltransf...   144   7e-36

>gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransferase
           component of pyruvate dehydrogenase complex
           [Herbaspirillum seropedicae SmR1]
          Length = 554

 Score =  575 bits (1481), Expect = e-165,   Method: Compositional matrix adjust.
 Identities = 279/314 (88%), Positives = 294/314 (93%), Gaps = 1/314 (0%)

Query: 9   SSVNKSHASPSVRKYGRELGVDLTRVVGTGPKNRILQEDIQKFVKAALAGA-GTAASAPA 67
           S+  K+HASPSVRK+ RELGVDL+RV  TGPK RILQ D+Q FVK  +AG+   AASAPA
Sbjct: 241 STGGKAHASPSVRKFARELGVDLSRVPATGPKGRILQLDVQNFVKGVMAGSTSVAASAPA 300

Query: 68  KNGSGVGLDLLPWPSLDFSKFGTTELLPLSRIKKISGPNLHRNWVMIPHVTQFDEADVTE 127
            NGSG G++LLPWPSLDFSKFG TEL PLSRIKKISGPNLHRNWVMIPHVTQFDEADVTE
Sbjct: 301 TNGSGAGMNLLPWPSLDFSKFGETELQPLSRIKKISGPNLHRNWVMIPHVTQFDEADVTE 360

Query: 128 LEEFRKTSNDAYAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKQYYNI 187
           LEEFRKTSNDA+AKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILK++YNI
Sbjct: 361 LEEFRKTSNDAFAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKKFYNI 420

Query: 188 GFAADTPNGLVVPVIKNADQKTIGQIATEMGELSAQARDGKLKPADMQGATFTISSLGGI 247
           GFAADTPNGLVVPVIKNADQK+IGQIA EMGELSAQARDGKLKPADMQGATFTISSLGGI
Sbjct: 421 GFAADTPNGLVVPVIKNADQKSIGQIAIEMGELSAQARDGKLKPADMQGATFTISSLGGI 480

Query: 248 GGTAFTPIINAPEVAILGLSKSIIKPVWDGKQFAPRLMLPTSLSYDHRVVDGAMGARFSA 307
           GGTAFTPIINAPEVAILGLSKSI KPVWDGKQF PRLMLPTSLSYDHRV+DGAMGARFSA
Sbjct: 481 GGTAFTPIINAPEVAILGLSKSITKPVWDGKQFVPRLMLPTSLSYDHRVIDGAMGARFSA 540

Query: 308 YLAEVLGDLRKSLL 321
           YLA+VLGDLRKSLL
Sbjct: 541 YLADVLGDLRKSLL 554


>gi|300312272|ref|YP_003776364.1| dihydrolipoamide
           succinyltransferase component of 2-oxoglutarate
           dehydrogenase complex protein [Herbaspirillum
           seropedicae SmR1]
          Length = 413

 Score =  144 bits (364), Expect = 7e-36,   Method: Compositional matrix adjust.
 Identities = 81/228 (35%), Positives = 130/228 (57%), Gaps = 3/228 (1%)

Query: 94  LPLSRIKKISGPNLHRNWVMIPHVTQFDEADVTELEEFRKTSNDAYAKAKSPVKLTMLAF 153
           +P+SR++      L ++      +T F+E ++  + + R    D + K    VKL  ++F
Sbjct: 187 VPMSRLRARVAERLVQSQSTNAILTTFNEINMQPVIDLRNKYKDKFEKEHG-VKLGFMSF 245

Query: 154 VIKASVSALKKFPAFNSSLDAKGENLILKQYYNIGFAADTPNGLVVPVIKNADQKTIGQI 213
            +KA V ALKK+P  N+S+D  G +++   Y++IG A  +P GLVVP++++ADQ +I  I
Sbjct: 246 FVKAVVHALKKYPIVNASVD--GNDIVYHGYFDIGVAVGSPRGLVVPILRDADQMSIADI 303

Query: 214 ATEMGELSAQARDGKLKPADMQGATFTISSLGGIGGTAFTPIINAPEVAILGLSKSIIKP 273
             ++GE   +A+DGKL   ++ G TFT+S+ G  G    TPIIN P+ AILG+  +  + 
Sbjct: 304 EKKIGEFGQKAKDGKLSIEELSGGTFTVSNGGTFGSMLSTPIINPPQSAILGIHATKERA 363

Query: 274 VWDGKQFAPRLMLPTSLSYDHRVVDGAMGARFSAYLAEVLGDLRKSLL 321
           V +  Q   R +   +LSYDHR++DG         + E L D  + LL
Sbjct: 364 VVENGQVVVRPINYFALSYDHRIIDGREAVLSLVAIKEALEDPARLLL 411


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.133    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 955,384
Number of extensions: 38368
Number of successful extensions: 122
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 120
Number of HSP's successfully gapped: 2
Length of query: 321
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 236
Effective length of database: 1,217,395
Effective search space: 287305220
Effective search space used: 287305220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 130 (54.7 bits)