BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0083.p (554 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3... 911 0.0 gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3... 295 6e-81 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 160 3e-40 gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransfer... 57 4e-09 >gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 911 bits (2354), Expect = 0.0, Method: Compositional matrix adjust. Identities = 489/554 (88%), Positives = 513/554 (92%), Gaps = 3/554 (0%) Query: 1 MEIPSSTAGVVKELKVKVGDKVAEGSVLLLVEASGDAAAAPAAPATAPAAAPAPAAVAPA 60 MEIPSS AGVVKE+KVKVGDKVAEGS+LLLVEA+G AAAAPA A AA A A A A Sbjct: 46 MEIPSSQAGVVKEVKVKVGDKVAEGSLLLLVEAAGQAAAAPAPAAPPAPAAAAAPAPAAA 105 Query: 61 PAAAPAAAAPAPVAASFGGSADIECDMMVLGAGPGGYSAAFRAADLGMNTVLVERYSTLG 120 PAAAP AA AASFGGSAD++CD+MVLG GPGGYSAAFRAADLG+NTV+VER +TLG Sbjct: 106 PAAAPTAAP---TAASFGGSADVQCDVMVLGGGPGGYSAAFRAADLGLNTVIVEREATLG 162 Query: 121 GVCLNVGCIPSKALLHVAAVIDETAAMASHGVTFAKPEIDIDKLRAYKEKVIGTMTGGLA 180 GVCLNVGCIPSKALLHVAAVIDETAAMASHGVTF KP+IDIDKLRAYK+KVIGTMTGGLA Sbjct: 163 GVCLNVGCIPSKALLHVAAVIDETAAMASHGVTFGKPQIDIDKLRAYKDKVIGTMTGGLA 222 Query: 181 GMAKARKVNVVQGVGQFVGANHIEVTAADGSKKTVKFKQAIIAAGSSVVNLPFVPQDPRI 240 GMAKARKV VV G GQF G NHIEVTAADGSKK V+FK AIIAAGSSVVNLPFVPQDPRI Sbjct: 223 GMAKARKVQVVNGDGQFAGPNHIEVTAADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPRI 282 Query: 241 VDSTGALELRQVPKRMLVIGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVW 300 VDSTGALELRQVPKRMLVIGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVW Sbjct: 283 VDSTGALELRQVPKRMLVIGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVW 342 Query: 301 QKFNEKRFDNVMLKTRTVGVEALKEGIKVTFEAAEAGAKAPEPQVYDLVLVAVGRSPNGK 360 QKFNEKRFDNVM+KT+TV VEAL EGIKVTFEAAEAGA APEPQ+YDLVLVAVGRSPNGK Sbjct: 343 QKFNEKRFDNVMVKTKTVAVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGK 402 Query: 361 KIAADKAGVTVTDRGFIPVDSQMRTNVPHIFAIGDLVGQPMLAHKAVHEAHVAAEAAAGE 420 K++ADKAGV V+DRGFI VD QMRTNVPHIFAIGDLVGQPMLAHKAVHE HVAAEA AGE Sbjct: 403 KLSADKAGVIVSDRGFIAVDKQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGE 462 Query: 421 KSHFDASVIPSVAYTDPEVAWVGITEDEAKAKGIKLEKGHFPWAASGRAVANGRSEGFTK 480 KS FDASVIPSVAYTDPEVAWVG+TEDEAKAKGIK+EKGHFPWAASGRAVANGRSEGFTK Sbjct: 463 KSFFDASVIPSVAYTDPEVAWVGVTEDEAKAKGIKIEKGHFPWAASGRAVANGRSEGFTK 522 Query: 481 LLFDAETHRIVGGGIVGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGMAAEV 540 LLFDAETHRI+GGGIVGTHAGDMIGE+ALAIEMGADAVDIGKTIHPHPTLGES+GMAAEV Sbjct: 523 LLFDAETHRIIGGGIVGTHAGDMIGEVALAIEMGADAVDIGKTIHPHPTLGESLGMAAEV 582 Query: 541 YKGVCTDLPPMRKK 554 +G CTDLPP RKK Sbjct: 583 AEGHCTDLPPQRKK 596 >gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 295 bits (755), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 172/452 (38%), Positives = 257/452 (56%), Gaps = 13/452 (2%) Query: 97 YSAAFRAADLGMNTVLVERYSTL------GGVCLNVGCIPSKALLHVAAVIDETA-AMAS 149 Y AA RAA LG NT ++ + GG C NVGCIPSKALL + + + A Sbjct: 17 YIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQSSEHYEHASHGFAE 76 Query: 150 HGVTFAKPEIDIDKLRAYKEKVIGTMTGGLAGMAKARKVNVVQGVGQFV--GANHIEVTA 207 HG+ ++++K+ K V+ G+ + K KV+ G G FV AN E+ Sbjct: 77 HGIEVKGLGLNLEKMLGRKNTVVKQNNDGILYLFKKNKVSFFHGRGSFVKGDANGYEIKV 136 Query: 208 ADGSKKTVKFKQAIIAAGSSVVNLPFVPQDPR-IVDSTGALELRQVPKRMLVIGGGIIGL 266 A +++T+ K I+A GS+ LP D + I+ +TGAL + +VPK++ VIG G+IGL Sbjct: 137 AGAAEETITAKHVIVATGSNARALPGAEFDEKLILSNTGALSITEVPKKLGVIGAGVIGL 196 Query: 267 EMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMLKTRTVGVEALKEG 326 EM +V+ LGA + V+E + + D + K QK K+ V L + ++A K Sbjct: 197 EMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLTKQGLAVNLGVKIGAIKAGKNS 256 Query: 327 IKVTFEAAEAGAKAPEPQVYDLVLVAVGRSPNGKKIAADKAGVTVTDRGFIPVDSQMRTN 386 + V + A+ A E +D ++V++GR+PN + A+ G+ + +RGFI VD +TN Sbjct: 257 VSVEYTDAKGDAHTGE---FDKLIVSIGRTPNTIGLNAEGVGLKLDERGFIAVDGDCKTN 313 Query: 387 VPHIFAIGDLVGQPMLAHKAVHEAHVAAEAAAGEKSHFDASVIPSVAYTDPEVAWVGITE 446 +P+++A+GD+V PMLAHKA E AE AG+ H + + IP V YT PE+AWVG TE Sbjct: 314 LPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTSPEIAWVGKTE 373 Query: 447 DEAKAKGIKLEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIVGGGIVGTHAGDMIGE 506 + KA+G++ + G FP+ A+GRA A G + G K L DA+T I+G IVG A ++I E Sbjct: 374 QQLKAEGVQYKAGTFPFLANGRARALGDTSGMVKFLADAKTDEILGVHIVGPMASELISE 433 Query: 507 IALAIEMGADAVDIGKTIHPHPTLGESIGMAA 538 +A+E A + DI + H HP+L E+ AA Sbjct: 434 AVVAMEFRASSEDIARICHAHPSLSEATKEAA 465 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 160 bits (405), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 131/462 (28%), Positives = 218/462 (47%), Gaps = 21/462 (4%) Query: 81 ADIECDMMVLGAGPGGYSAAFRAADLGMNTVLVERYSTLGGVCLNVGCIPSKALLHVAAV 140 + + D+ +G G GG AA A+ G L E+ LGG C+N+GCIP K + + A Sbjct: 2 SQYDYDLFTIGGGSGGVRAARFASQAGARVGLAEK-GDLGGTCVNLGCIPKKLMSYSAHY 60 Query: 141 IDETAAMASHGVTF-AKPEIDIDKLRAYKEKVIGTMTGGLAGMAKARKVNVVQGVGQFVG 199 +E A A +G T +P D L A K++ I + + KV++ +G + Sbjct: 61 HEEFADAAGYGWTLNGQPSFDWSALMANKDREIAHLNDIYLRLLDNAKVSLHRGFAKVED 120 Query: 200 ANHIEVTAADGSKKTVKFKQAIIAAGSSVVNLPFVPQDPRIVDSTGALELRQVPKRMLVI 259 A+ + V DG + T + I+ A + P +P + S L+ +P+R +V+ Sbjct: 121 AHTVNV---DGQRFTARH---ILVATGGRPDKPAIPGAELGITSDDFFHLKALPQRAVVL 174 Query: 260 GGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMLKTRTVG 319 GGG I +E+A++ + LG + +V + L++ D D+ K+ ++ Sbjct: 175 GGGYIAVELASILNGLGCEVTLVYRRERLLRNMDADLGIHLADEMAKKGIRIVFNASIEA 234 Query: 320 VEALKEGIKVTFEAAEAGAKA-----PEPQVYDLVLVAVGRSPNGKKIAADKAGVTVTDR 374 +EA + G EA+EA K E VL A GR+ N + +AGV + Sbjct: 235 IEAEQAG-----EASEASVKTVRLTNGEALSAQCVLFATGRTANTAGLGLQEAGVKLKAN 289 Query: 375 GFIPVDSQMRTNVPHIFAIGDLVGQPMLAHKAVHEAH--VAAEAAAGEKSHFDASVIPSV 432 G I V+ ++VP I A+GD++ + L A+ EA V+ GE+ A+ IP+ Sbjct: 290 GAIEVNQDFESSVPSILAVGDVIDRVALTPVALAEAMAVVSRLFGKGERGMSYAN-IPTA 348 Query: 433 AYTDPEVAWVGITEDEAKAKGIKLEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIVG 492 ++ P V VG++E+EA+ + +L + ++ F KL+ DA T R++G Sbjct: 349 VFSHPNVGTVGLSEEEARQQFGELRIFKTDFKPLKNTLSRNTERTFMKLVVDARTDRVLG 408 Query: 493 GGIVGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESI 534 +VG AG++I A+A++ GA TI HPT E Sbjct: 409 VHMVGGDAGEVIQGFAVALQCGATKAQFDTTIGIHPTSAEEF 450 >gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex [Herbaspirillum seropedicae SmR1] Length = 554 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 28/35 (80%), Positives = 32/35 (91%) Query: 1 MEIPSSTAGVVKELKVKVGDKVAEGSVLLLVEASG 35 MEIPSS AGVVKE+KVKVGDKVAEGS+L++VE G Sbjct: 46 MEIPSSQAGVVKEIKVKVGDKVAEGSLLVIVEGEG 80 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,499,350 Number of extensions: 58501 Number of successful extensions: 315 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 6 Length of query: 554 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 464 Effective length of database: 1,193,720 Effective search space: 553886080 Effective search space used: 553886080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)