BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0082.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 464 e-132 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 125 6e-30 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 117 9e-28 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 115 4e-27 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 102 5e-23 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 464 bits (1193), Expect = e-132, Method: Compositional matrix adjust. Identities = 221/309 (71%), Positives = 258/309 (83%) Query: 1 MGMSDFYSNRDDAESIATIHRALELGICFLDTADMYGPHINEELIAKAIKGKRDQVFIAT 60 MGMSDFY+ RDDAES+ATI RALELGI LDTADMYGP+ NEEL+ +AIKG+RD+ FIAT Sbjct: 35 MGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEELLGRAIKGRRDKFFIAT 94 Query: 61 KFGIVRDPANPAARGVNGKPDYIRTSVEGSLKRLGVETIDLYYQHRIDQSTPIEETVGAL 120 KFGI RDP++P ARGV+G P YIR +VEGSLKRLGVETIDLYYQHRID +TPIE T+G L Sbjct: 95 KFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDLYYQHRIDPATPIEVTMGVL 154 Query: 121 ADLVKAGKIRHIGLSEASSQTLERASKVHKITALQSEYSLWTRDPEQGVLATCNKLGIGF 180 ADLV+AGKIR+IGLSEAS+ TLERA +VH +TALQSEYSLWTRDPE VLA C LGIGF Sbjct: 155 ADLVQAGKIRYIGLSEASAATLERAHRVHPVTALQSEYSLWTRDPEAEVLAACRTLGIGF 214 Query: 181 VPYSPLGRGFLTGAITRPDQFEADDYRRHNPRFQGENFAKNLLLVEKVKELATRYECSPS 240 V YSPLGRGFLTGAI R + DD+RR NPRFQGENFA+NL L EKVKE+A ++ C+PS Sbjct: 215 VAYSPLGRGFLTGAIQRFEDLAEDDFRRSNPRFQGENFARNLQLAEKVKEMAGQHGCTPS 274 Query: 241 QLALAWVLAQGDHIVPIPGTKRRTYLEENSGALAVTLTASHLEELNRLFPVDAAAGPRYT 300 QLALAWV+AQ HIVPIPGTKRR YLE+N+G++ V L L+ L+ +FP AAAG RYT Sbjct: 275 QLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVKLAPEDLQALDAIFPRGAAAGERYT 334 Query: 301 EEGMRIING 309 + M+++NG Sbjct: 335 DASMKMLNG 343 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 125 bits (313), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 93/295 (31%), Positives = 138/295 (46%), Gaps = 27/295 (9%) Query: 1 MGMSDFYSNRDDAESIATIHRALELGICFLDTADMYGPHINEELIAKAIKGKRDQVFIAT 60 +G F D ES + A E G+ F+DTAD+Y +EE++ K + G+R + +A+ Sbjct: 18 LGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYTKGKSEEIVGKLLAGQRHEWVLAS 77 Query: 61 KFGIVRDPANPAARGVNGKPD-------YIRTSVEGSLKRLGVETIDLYYQHRIDQSTPI 113 K G ++GKP+ +I E L RL + +D+ Y HR Q + Sbjct: 78 KLG----------NAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILYMHRDYQDENL 127 Query: 114 EETVGALADLVKAGKIRHIGLSE------ASSQTLERASKVHKITALQSEYSLWTRDPEQ 167 EE V AL DL++ GKIR G+S A L + V Q Y+L R PE Sbjct: 128 EEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEV 187 Query: 168 GVLATCNKLGIGFVPYSPLGRGFLTGAITRPDQFEADDYR--RHNPRFQGENF-AKNLLL 224 +L C G+G VPYSP+ RG LTG +P Q A D R R + R F ++LL+ Sbjct: 188 EILPACAHYGLGVVPYSPIARGVLTGKY-QPGQKPAQDTRAGRGDKRMMETEFREESLLI 246 Query: 225 VEKVKELATRYECSPSQLALAWVLAQGDHIVPIPGTKRRTYLEENSGALAVTLTA 279 +++++ Q A AWVLA I G + +E+ A + ++A Sbjct: 247 AQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKISA 301 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 117 bits (294), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 81/264 (30%), Positives = 135/264 (51%), Gaps = 23/264 (8%) Query: 1 MGMSDFYSNRDDAES-IATIHRALELGICFLDTADMYGPHINE------ELIAKAIKGKR 53 +G+ + D+ E+ A + RA + G+ D A+ YGP +AK R Sbjct: 31 LGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPPAGSAEENFGRWMAKDFLPYR 90 Query: 54 DQVFIATKFGIVRDPANPAARGVNGKPDYIRTSVEGSLKRLGVETIDLYYQHRIDQSTPI 113 D++ I++K G P G +G ++ S E SLKR+G++ +D++Y HR+D TP+ Sbjct: 91 DELVISSKAGWQMWPGPYG--GPSGARKHLIASCEQSLKRMGLDYVDIFYSHRVDADTPL 148 Query: 114 EETVGALADLVKAGKIRHIGLSEASSQTLERASKVHKITAL-----QSEYSLWTRDPEQG 168 EET+GALA L + GK ++G+S S + +A+ + K + Q YS+ R E G Sbjct: 149 EETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEGVPLLIHQPNYSMLNRAIEGG 208 Query: 169 VLATCNKLGIGFVPYSPLGRGFLT----GAITRPDQFEADDYRRHNPRFQGENFAKNLLL 224 +L +LG+G + +SPL +G LT G + P+ R E+ NL Sbjct: 209 LLPALEELGVGCIGFSPLAQGLLTSKYLGGV--PEGARVTRAPSLPQRMLSED---NLAR 263 Query: 225 VEKVKELATRYECSPSQLALAWVL 248 + + E+A++ + +Q+A+AWVL Sbjct: 264 IRALNEIASKRGQTLAQMAIAWVL 287 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 115 bits (289), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 94/294 (31%), Positives = 146/294 (49%), Gaps = 24/294 (8%) Query: 17 ATIHRALELGICFLDTADMYGPHINEELIAKAIKGKR-DQVFIATKFGIVRDPANPAARG 75 A +H AL+ G+ F+DTAD+YG +E+LIA+ +K + ++ F+ATK G R + A G Sbjct: 37 AALHAALDAGVSFIDTADVYGDGRSEQLIAQVLKERGGERPFVATKAG--RRLSPHVASG 94 Query: 76 VNGKPDYIRTSVEGSLKRLGVETIDLYYQHRIDQSTPIE-----ETVGALADLVKAGKIR 130 N + + ++ SLK LGV+ +DL H P E E G L LV AGKIR Sbjct: 95 YNDRAQ-LSAFIDRSLKNLGVDCLDLVQLH----CPPTEVYYQQEVFGHLDALVAAGKIR 149 Query: 131 HIGLSEASSQTLERASKVHKITALQSEYSLWTRDPEQGVLATCNKLGIGFVPYSPLGRGF 190 H G+S + +A + + ++Q ++++ + P L + I + PL G Sbjct: 150 HYGVSVEKVEEALKAIEYPNVKSVQIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGL 209 Query: 191 LTGAITRPDQFEADDYR---RHNPRFQ-GENFAK-----NLLLVEKVKELATRYECSPSQ 241 LTG ++ F ADD+R RH F GE F+ L VEK++ L + +Q Sbjct: 210 LTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGVPYEVALAAVEKIRALVPQ-GVPMAQ 268 Query: 242 LALAWVLAQGDHIVPIPGTKRRTYLEENSGALAV-TLTASHLEELNRLFPVDAA 294 AL W+L + IPG + + NS A A+ +TA ++ L ++ D A Sbjct: 269 FALRWILMESGISTVIPGARNVAQAQSNSAASALPPITAEVMQALRAIYLHDIA 322 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 102 bits (253), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 91/288 (31%), Positives = 139/288 (48%), Gaps = 47/288 (16%) Query: 1 MGMSDFYSNRDDAESIATIHRALELGICFLDTADMY-------GPHINEELIAKAIK--G 51 +G + +A++ A + A+E GI +D A+MY + E + +K G Sbjct: 18 LGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAETQGLTERYLGTWLKKSG 77 Query: 52 KRDQVFIATKF-GIVRDPANPAARGVNGKPDYI-----RTSVEGSLKRLGVETIDLY--- 102 KRDQ+ IATK G R P NP R V G + + ++ GSL+RL ++ +DLY Sbjct: 78 KRDQLLIATKCTGPARKPHNP--RHVRGGINNLDRKGLTEALHGSLERLQLDHVDLYQLH 135 Query: 103 -------------YQHRIDQST-PIEETVGALADLVKAGKIRHIGLSEASSQTLE---RA 145 YQH D+ST PIEET+ L++ VKAGKIR IGLS ++ + RA Sbjct: 136 WPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIGLSNETAWGVAQFLRA 195 Query: 146 SKVH---KITALQSEYSLWTRDPEQGVLATCNKLGIGFVPYSPLGRGFLTGAI---TRPD 199 ++ H +I +Q+ Y+L R E + ++ +G + YSPL G L+G RP Sbjct: 196 AEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLAFGMLSGKYLDGARPA 255 Query: 200 QFEADDYRRHNPRFQGENFAKNLLLVEKVKELATRYECSPSQLALAWV 247 + RF A+ + LA ++ P+Q+ALA+V Sbjct: 256 GGRLTLFE----RFSRYTNAQAERATAEYVALARQHGLDPAQMALAYV 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 959,070 Number of extensions: 38935 Number of successful extensions: 135 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 6 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)