BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0073.p (575 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 1061 0.0 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 755 0.0 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 437 e-124 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 243 3e-65 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 176 5e-45 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 1061 bits (2743), Expect = 0.0, Method: Compositional matrix adjust. Identities = 504/575 (87%), Positives = 544/575 (94%), Gaps = 2/575 (0%) Query: 3 DTKTFTRRSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFR 62 DTK TRRSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFR Sbjct: 4 DTKKSTRRSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFR 63 Query: 63 ALAEQVKIGIWEAGGFPLEFPVMSLGETLMRPTAMLFRNLASMDVEESIRANPLDGVVLL 122 ALAEQVKIGIWEAGGFPLEFPVMSLGETL+RPTAML+RNLASMDVEESIRANPLDGVVLL Sbjct: 64 ALAEQVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGVVLL 123 Query: 123 MGCDKTTPALLMGASSVDLPTIGISGGPMLSGKYRGGELGSGTGVWQMSEEVRAGQMSQE 182 MGCDKTTPALLMGA+SVD+PTIG+SGGPMLSGKYRG ELGSGTGVWQMSE+VRAG+M+QE Sbjct: 124 MGCDKTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKMTQE 183 Query: 183 EFFEAESCMHRSHGHCMTMGTASTMASMVEALGMSLPGNAAIPAVDARRNVLARNSGRRI 242 EFFEAESCMHRSHGHCMTMGTASTMASMVEALG+ LP NAAIPAVDARRNVLARN+GRRI Sbjct: 184 EFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRI 243 Query: 243 VEMVKEDLIMSRILTKAAFENAIRVNAGIGGSTNAVIHLLAIAGRIGVDLKLEEWDKIGQ 302 V+MV+EDL++S+ILT+ AFENAIRVNA IGGSTNAVIHLLAIAGRIG+ L L++WD IGQ Sbjct: 244 VQMVEEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDIGQ 303 Query: 303 KLPCLVNLQPSGKYLMEDFYYAGGLPSVIRELESVIDKSALSANGQTLWDNCKDAPNWNR 362 +LPCL++LQPSGK+LMEDFYYAGGLP+VIR+LESVIDK+AL+ANG+TLW+NC+DAPNWN Sbjct: 304 QLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESVIDKTALTANGKTLWENCQDAPNWNE 363 Query: 363 DVIHAFDTPFKAAAGIAILHGNLCPDGAVIKPSAATPALLKHTGRAVVFENSDDLHKRID 422 +VI +FD PFK AAGIAIL GNL PDGAVIKPSAATPALLKH GRAVVFENSDDLHKRID Sbjct: 364 EVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRID 423 Query: 423 DENLDVDENCVLVLKNCGPKGYPGMGEAGNMPLPPKVLRKGITDMVRVSDARMSGTAYGT 482 DENLDVDE CVLVLKNCGPKGYPGM EAGNMPLPPK+LRKGITDMVRVSDARMSGTAYGT Sbjct: 424 DENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTAYGT 483 Query: 483 VVLHVTPEAAAGGPLALVQDGDLVELDVDARKLHLHVSDEELARRRALWQAPKAPA--DR 540 VVLHV+PEAAAGGPLA+VQ+GD +ELDV+ARKLHL VSDEELARRRA WQ P+ P R Sbjct: 484 VVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHLDVSDEELARRRAQWQKPELPPQMQR 543 Query: 541 GWVKLYVDHVQQANLGADLDFLVGKSGSGIPKDNH 575 GWVKLYVDHVQQAN GADLDFLVGKSG +PKDNH Sbjct: 544 GWVKLYVDHVQQANQGADLDFLVGKSGPYVPKDNH 578 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 755 bits (1950), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/578 (64%), Positives = 453/578 (78%), Gaps = 7/578 (1%) Query: 5 KTFTRRSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFRAL 64 K+ T RS WFG D++GF+YRSW+KN+GIP +F G+PVIGICNT+SELTPCN+HFR + Sbjct: 8 KSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKI 67 Query: 65 AEQVKIGIWEAGGFPLEFPVMSLGETLMRPTAMLFRNLASMDVEESIRANPLDGVVLLMG 124 AE V+ GI EAGGFP+EFPV S GE+ +RPTAML RNLASMDVEESIR NP+D VVLL G Sbjct: 68 AEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPIDAVVLLTG 127 Query: 125 CDKTTPALLMGASSVDLPTIGISGGPMLSGKYRGGELGSGTGVWQMSEEVRAGQMSQEEF 184 CDKTTPALLMGA+S D+P I ++GGPML+GK++G ++GSGT VWQ+SE+V+AG+++ +F Sbjct: 128 CDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAGEITIHDF 187 Query: 185 FEAESCMHRSHGHCMTMGTASTMASMVEALGMSLPGNAAIPAVDARRNVLARNSGRRIVE 244 AE+ M RS G C TMGTASTMA M E+LG+SLP NAAIPAVDARR VLA SG RIV+ Sbjct: 188 MAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIVD 247 Query: 245 MVKEDLIMSRILTKAAFENAIRVNAGIGGSTNAVIHLLAIAGRIGVDLKLEEWDKIGQKL 304 MV E L +S+ILT+ AFENAIR NA IGGSTNAVIHL AIAGRIGVDL+LE+W +IG+ Sbjct: 248 MVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGT 307 Query: 305 PCLVNLQPSGKYLMEDFYYAGGLPSVIREL---ESVIDKSALSANGQTLWDNCKDAPNWN 361 P +V+LQPSG+YLME+FYYAGGLP+V+R L + + K AL+ NGQT+WDN KDAP +N Sbjct: 308 PTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNVKDAPIYN 367 Query: 362 RDVIHAFDTPFKAAAGIAILHGNLCPDGAVIKPSAATPALLKHTGRAVVFENSDDLHKRI 421 +V+ P GI IL GNL P GAV+KPSAATP L+KH GRAVVFE+ + +RI Sbjct: 368 DEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERI 427 Query: 422 DDENLDVDENCVLVLKNCGPKGYPGMGEAGNMPLPPKVLRKGITDMVRVSDARMSGTAYG 481 +D +LDVD +CVLV+KN GPKGYPGM E GNM LPPKVL G+ DMVR+SDARMSGTAYG Sbjct: 428 NDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDARMSGTAYG 487 Query: 482 TVVLHVTPEAAAGGPLALVQDGDLVELDVDARKLHLHVSDEELARR---RALWQAPKAPA 538 TV+LHV PEAAAGGPL +VQDGD +ELD A KL L +S+EE+ RR RA A + P Sbjct: 488 TVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKVLAERKPE 547 Query: 539 DR-GWVKLYVDHVQQANLGADLDFLVGKSGSGIPKDNH 575 G+ LYVD V QA+ G D DFLVG G+ +PK +H Sbjct: 548 MVGGYQSLYVDRVLQADEGCDFDFLVGCRGAAVPKHSH 585 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 437 bits (1125), Expect = e-124, Method: Compositional matrix adjust. Identities = 243/584 (41%), Positives = 356/584 (60%), Gaps = 19/584 (3%) Query: 10 RSQAWFGRQDRDGF--IYRSWVKNRGIPHDQF-DGRPVIGICNTYSELTPCNSHFRALAE 66 RSQ WF D +Y N GI ++ GRP+IGI + S+++PCN LA+ Sbjct: 12 RSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLELAK 71 Query: 67 QVKIGIWEAGGFPLEFPVMSLGETLMRPTAMLFRNLASMDVEESIRANPLDGVVLLMGCD 126 +V+ GI +AGG P+EFP+ + E RPTA + RNLA + + E + P+D VVL GCD Sbjct: 72 RVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLTTGCD 131 Query: 127 KTTPALLMGASSVDLPTIGISGGPMLSGKYRGGELGSGTGVWQMSEEVRAGQMSQEEFFE 186 KTTP+ +M A++VD+P I +SGGPML G G +GSG+ +W+ + + AG + E+F E Sbjct: 132 KTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEKFLE 191 Query: 187 AESCMHRSHGHCMTMGTASTMASMVEALGMSLPGNAAIPAVDARRNVLARNSGRRIVEMV 246 + S GHC TMGTASTM +M EALGMSL G +AIPA R +A +GRRIV M Sbjct: 192 IAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA 251 Query: 247 KEDLIMSRILTKAAFENAIRVNAGIGGSTNAVIHLLAIAGRIGVDLKLEEWDKIGQKLPC 306 EDL S ILT+ AF +AI VNA IGGSTNA H++A+A GV+L+ E+W K G +P Sbjct: 252 YEDLRPSAILTRDAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPL 311 Query: 307 LVNLQPSGKYLMEDFYYAGGLPSVIRELESV--IDKSALSANGQTLWDNCKDAPNWNRDV 364 L+N+QP+GKYL E F+ AGG+P+++ EL+ + ++A G+T+ +N + + +R++ Sbjct: 312 LLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRASNDREM 371 Query: 365 IHAFDTPFKAAAGIAILHGNLCPDGAVIKPSAATPAL----LKHTG-------RAVVFEN 413 I+ F P + AG +L GNL D A++K S + L G RAVVF+ Sbjct: 372 IYPFAAPLRERAGFLVLKGNLF-DFAIMKTSVISETFRERYLSTPGQENIFECRAVVFDG 430 Query: 414 SDDLHKRIDDENLDVDENCVLVLKNCGPKGYPGMGEAGNMPLPPKVLRKGITDMVRVSDA 473 SDD H RI+D L +DEN +L ++ GP G+PG E NM P ++++G++ + + D Sbjct: 431 SDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTLPTLGDG 490 Query: 474 RMSGTAYGTVVLHVTPEAAAGGPLALVQDGDLVELDVDARKLHLHVSDEELARRRALWQA 533 R SGT+ +L+ +PE+A GG LA ++DGD V +D++ + ++ VS+EELARR++ Sbjct: 491 RQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELARRKSEGIP 550 Query: 534 PKAPADRGWVKLYVDHVQQANLGADLDFLVGKSG--SGIPKDNH 575 P P+ W ++Y V Q GA ++ + G +P+ NH Sbjct: 551 PVPPSQTPWQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH 594 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 243 bits (620), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 173/590 (29%), Positives = 299/590 (50%), Gaps = 69/590 (11%) Query: 12 QAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFRALAEQVKIG 71 +++ Q R+ R+ + G+ F +P+I + N++++ P + H + L + V Sbjct: 5 RSYTTTQGRNMAGARALWRATGMKDGDFT-KPIIAVVNSFTQFVPGHVHLKDLGQLVARE 63 Query: 72 IWEAGGFPLEFPVMSLGETLMRPTAMLFRNLASMD-----VEESIRANPLDGVVLLMGCD 126 I AGG EF +++ ++ A + +L S D VE + A+ D +V + CD Sbjct: 64 IEAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCD 123 Query: 127 KTTPALLMGASSVDLPTIGISGGPMLSGKYRGGELGSGTGVWQMS---EEVRAG--QMSQ 181 K TP +LM A +++P + +SGGPM +GK + + V ++ ++AG +S Sbjct: 124 KITPGMLMAAMRLNIPVVFVSGGPMEAGKVIK-TVNTDKKVIKLDLVDAMIQAGDKNISD 182 Query: 182 EEFFEAESCMHRSHGHCMTMGTASTMASMVEALGMSLPGNAAIPAVDARRNVLARNSGRR 241 + E E + G C M TA++M + E LG+SLPGN I A A R L +GR Sbjct: 183 ADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRL 242 Query: 242 IVEMVKE-------DLIMSRILTKAAFENAIRVNAGIGGSTNAVIHLLAIAGRIGVDLKL 294 IVE+ K ++ I TK +ENA+ ++ +GGSTN V+HLLA A V+ + Sbjct: 243 IVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFTM 302 Query: 295 EEWDKIGQKLPCLVNLQP-SGKYLMEDFYYAGGLPSVIREL--ESVIDKSALSANGQTLW 351 + D+I +++PCL + P + KY +ED + AGG+ S++ EL ++D S + + +TL Sbjct: 303 ADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKTLG 362 Query: 352 DNCK-----------------------------------DAPNWNR--DVIHAFDTPFKA 374 + + +A + +R I + + Sbjct: 363 EAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAYSQ 422 Query: 375 AAGIAILHGNLCPDGAVIKPSAATPALLKHTGRAVVFENSDDLHKRIDDENLDVDENCVL 434 G+A+L+GN+ G ++K + ++LK +G A VFE+ D + I + + + V+ Sbjct: 423 DGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGD--VV 480 Query: 435 VLKNCGPKGYPGMGEAGNMPLPPKVLR-KGITDMVRV-SDARMSGTAYGTVVLHVTPEAA 492 +++ GPKG PGM E M P ++ KG+ + +D R SG + G V+ H +PEAA Sbjct: 481 IVRYEGPKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAA 537 Query: 493 AGGPLALVQDGDLVELDVDARKLHLHVSDEELARRRALWQAPKAPADRGW 542 GG + LV++GD++++D+ R ++L +S ++LA+RR A +A D W Sbjct: 538 EGGAIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRT---AMEARGDAAW 584 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 176 bits (446), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 155/523 (29%), Positives = 238/523 (45%), Gaps = 48/523 (9%) Query: 40 DGRPVIGICNTYSELTPCNSHFRALAEQVKIGIWEAGG---FPLEFPVMSLGETLMRP-- 94 D P + I + Y+++ + F + +K EAG F P M G T + Sbjct: 65 DKAPSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQAGM 124 Query: 95 -TAMLFRNLASMDVEESIRANPLDGVVLLMGCDKTTPALLMGASSV-DLPTIGISGGPML 152 ++ R+ +M ++ N D + L CDK P LL+GA LP + + GPM Sbjct: 125 ELSLFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMT 184 Query: 153 SGKYRGGELGSGTGVWQMSEEVRAGQMSQEEFFEAESCMHRSHGHCMTMGTASTMASMVE 212 SG S + ++ + AG++ + E EAES + G C GTA++ ++E Sbjct: 185 SGM-------SNSDKVKIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLME 237 Query: 213 ALGMSLPGNAAIPAVDARRNVLARNSGR---RIVEMVKEDLIMSRILTKAAFENAIRVNA 269 A+G+ LPG A I R+ L + + +I ++ + + R++ + + NAI Sbjct: 238 AMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQYTPVGRMIDEKSIVNAIVALH 297 Query: 270 GIGGSTNAVIHLLAIAGRIGVDLKLEEWDKIGQKLPCLVNLQPSGKYLMEDFYYAGGLPS 329 GGSTN +HL+AIA G+ + +++DK+ +P + + P+G + F+ AGG Sbjct: 298 ATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGF 357 Query: 330 VIREL--ESVIDKSALSANGQTLWDNCK-------------------DAPNWNRD--VIH 366 V+REL ++ + GQ L +CK DAP + D V+ Sbjct: 358 VLRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEGVLG 417 Query: 367 AFDTPFKAAAGIAILHGNLCPDGAVIKPSAATPALLKHTGRAVVFENSDDLHKRIDDENL 426 PF A G+ +L GNL AVIK SA P AVVF + + L Sbjct: 418 TVAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGQL 475 Query: 427 DVDENCVLVLKNCGPKGYPGMGEAGNMPLPPKVLRKGITDMVRVSDARMSGTAYGTV--V 484 D D V+ + GP+ GM E + +L+ + V+D RMSG A G V Sbjct: 476 DRDFVAVITFQ--GPRAN-GMPELHALTPALGILQDKGRHVALVTDGRMSG-ASGKVPAA 531 Query: 485 LHVTPEAAAGGPLALVQDGDLVELDVDARKLHLHVSDEELARR 527 +HVTPE GGPL V+DGD++ LD +A L V E A R Sbjct: 532 IHVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDATEWAAR 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,975,198 Number of extensions: 89538 Number of successful extensions: 249 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 233 Number of HSP's successfully gapped: 6 Length of query: 575 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 485 Effective length of database: 1,193,720 Effective search space: 578954200 Effective search space used: 578954200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)