BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0064.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferas... 666 0.0 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 152 4e-38 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 130 2e-31 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 128 1e-30 gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 127 2e-30 >gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 666 bits (1719), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/399 (80%), Positives = 352/399 (88%) Query: 1 MEFNEFAINKLMYVTNRPEIVFTEGSGMWMTDHTGKRYLDYLQGWAVNALGHAPQCISDA 60 M++++ NKLMYVTNRPE+VFTEG GMW+TDH GKRYLDYLQGWAVN LGHAPQCI+DA Sbjct: 1 MQYSQSDANKLMYVTNRPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGHAPQCIADA 60 Query: 61 LVAQGKKLINPSPAFYNAPSIELANLLTENSVFDRVFFTNSGAEANEGAFKLARKWGKLN 120 L AQ KKLINPSPAFYN PSIELA LLT NSVFDRVFF NSG EANEGA KLARKWGK N Sbjct: 61 LAAQSKKLINPSPAFYNEPSIELAKLLTANSVFDRVFFANSGGEANEGAIKLARKWGKKN 120 Query: 121 KNSAGEDRFEIITFNHSFHGRTIATMSASGKPGWDTMFAPQVPGFPKAELNDLASVERLI 180 + G RFEIITF HSFHGRT+ATMSASGK GWDTMFAPQVPGFPKA LNDL SV+ LI Sbjct: 121 PAADGSARFEIITFKHSFHGRTLATMSASGKDGWDTMFAPQVPGFPKAVLNDLESVKALI 180 Query: 181 SKKTVAVMLEPVQGEGGVIPATREFMQGLRRLTKNANLLLIVDEVQTGVGRTGELFGYQL 240 + TVAVMLEPVQGEGGVIPA++EFMQGLR LTK NLLLIVDEVQ+G+GRTG+LF YQ Sbjct: 181 GEHTVAVMLEPVQGEGGVIPASKEFMQGLRSLTKEKNLLLIVDEVQSGMGRTGQLFAYQH 240 Query: 241 SGIEPDIMTLGKGIGGGVPLAALLAREEIACFEAGEQGGTYNGNPLMTAVGAAVIKELLK 300 SGIEPDIMTL KGIGGGVPLAALLAREEIACFEAGEQGGTYNGNPLMTAVG AVIKELLK Sbjct: 241 SGIEPDIMTLAKGIGGGVPLAALLAREEIACFEAGEQGGTYNGNPLMTAVGVAVIKELLK 300 Query: 301 PDFLPAVKATGAYLSAELIKLSDKHGFEGERGEGLLRALKLGKDIGPQIVEAARDLNPVG 360 P F+ +V+ G YL +++S+K+GFEGERGEGLLRAL+LG+DIGPQIVEAAR+L PVG Sbjct: 301 PGFMESVRERGQYLRQRSLEISEKYGFEGERGEGLLRALQLGRDIGPQIVEAARNLEPVG 360 Query: 361 LLLNSPRPNLIRFMPSLNVTKDEIDQMLTMLEGVLVKLG 399 LLLNSPRPNL+RFMP+LNVTK+EIDQM +MLE VL K+G Sbjct: 361 LLLNSPRPNLLRFMPALNVTKEEIDQMFSMLEEVLAKIG 399 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 152 bits (384), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 134/398 (33%), Positives = 192/398 (48%), Gaps = 50/398 (12%) Query: 28 MWMTDHTGKRYLDYLQGWAVNALGHAPQCISDALVAQGKKLINPSPAFY-NAPSIELA-- 84 +W D G+R++D+ G AV GH + DA+ AQ K + + A +ELA Sbjct: 38 LW--DVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKFTHTAYQIVPYASYVELAER 95 Query: 85 -NLLTENSVFDRVFFTNSGAEANEGAFKLARKWGKLNKNSAGEDRFEIITFNHSFHGRTI 143 N LT + + F ++GAEA E A K+AR A R +I F FHGRT+ Sbjct: 96 INRLTPGNYPKKTAFFSTGAEAVENAIKIAR---------AHTGRPGVIAFAGGFHGRTM 146 Query: 144 ATMSASGK-----------PGWDTMFAPQVPGFPKA-----ELNDLASVERLI-----SK 182 M+ +GK PG D AP +P A + L +V+ L +K Sbjct: 147 MGMALTGKVAPYKLGFGPFPG-DVFHAP----YPSALHGITSEDALEAVKGLFKSDIEAK 201 Query: 183 KTVAVMLEPVQGEGGVIPATREFMQGLRRLTKNANLLLIVDEVQTGVGRTGELFGYQLSG 242 + A++LEPVQGEGG A +FM+GLR L +LLI DEVQ+G GRTG+LF + Sbjct: 202 RVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQSGYGRTGKLFAMEHYD 261 Query: 243 IEPDIMTLGKGIGGGVPLAALLAREEIACFEA-GEQGGTYNGNPLMTAVGAAVIKELLKP 301 + PD+MT+ K + GG+PL+A+ R EI A G GGTY GNPL A AV+ + + Sbjct: 262 VLPDLMTMAKSLAGGMPLSAVNGRAEIMDAPAPGGLGGTYAGNPLAIASALAVLDVMEEE 321 Query: 302 DFLPAVKATGAYLSAELIKLSDKHGFEGE-RGEGLLRALKLGKD-IGPQIVEAARDL--- 356 + + G L L +L E RG G + A++ G E + + Sbjct: 322 QLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAVEFADPATGKPDAEYTKKVQQH 381 Query: 357 ---NPVGLLLNSPRPNLIRFMPSLNVTKDEIDQMLTML 391 N + LL N+IRF+ L + +D+ L +L Sbjct: 382 ALNNGLLLLTCGSYGNVIRFLFPLTIPDTVMDEALGIL 419 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 130 bits (326), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 118/415 (28%), Positives = 188/415 (45%), Gaps = 71/415 (17%) Query: 17 RPEIVFTEGSGMWMTDHTGKRYLDYLQGWAVNALGHAPQCISDALVAQGKKLINPSPAFY 76 R I + G+ D G ++D G V+++G + +A+ Q + L++ S Sbjct: 46 RMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMH-SMEVN 104 Query: 77 NAPSIELANLLTE--------NSVFDRVFFTNSGAEANEGAFKLARKWGKLNKNSAGEDR 128 ++ ELA ++E + + FFT G++A E A K A++ R Sbjct: 105 SSKRTELAAKISEIMPDGLRGDCI---TFFTQGGSDALEAAVKFAKRV---------TGR 152 Query: 129 FEIITFNHSFHGRTIATMSASGKPGWDTMFAPQVPG-----------FP------KAE-- 169 +II F+ +HG A+ + + + F P + G FP AE Sbjct: 153 HQIIAFHGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQI 212 Query: 170 ----LNDLASVERLISKKTVAVMLEPVQGEGGVIPATREFMQGLRRLTKNANLLLIVDEV 225 ++ L + + AV++EPVQGEGG +P + EF+Q LR+ + LLIVDEV Sbjct: 213 AGEYVDYLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEV 272 Query: 226 QTGVGRTGELFGYQLSGIEPDIMTLGKGIGGGVPLAALLAREEIAC-FEAGEQGGTYNGN 284 Q G GRTG+++ + SG++PD++T GKGIGG +P+A L+ R ++A G Q T+ N Sbjct: 273 QAGAGRTGKMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKIPDGSQPNTFAAN 332 Query: 285 PLMTAVGAAVIKELLKPDFLPAVKATGAYLSAELIKLSDKHGFEGE-RGEGLLRALKLGK 343 + AV I L P +A L A+ S + GE RG GL+ Sbjct: 333 SISAAVALTNISILQDPRLDLVNRAHTLGLEAQERIRSFNSPWVGEVRGRGLM------- 385 Query: 344 DIGPQIVEAARDLNPVG----------------LLLNSPR-PNLIRFMPSLNVTK 381 IG ++VE P+ L++ R N++R MPSL + + Sbjct: 386 -IGIELVENRETREPLSREKLGKLMDYVVGHGVLMIPCGRYTNVMRVMPSLTIPR 439 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 104/350 (29%), Positives = 165/350 (47%), Gaps = 36/350 (10%) Query: 25 GSGMWMTDHTGKRYLDYLQGWAVNALGHAPQCISDALVAQGKKLINPSPAFYN-APSIEL 83 G GM + D GKRY+D G AV+ LGH + +A+ Q +L +F+ AP+ EL Sbjct: 19 GEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSFFTTAPAEEL 78 Query: 84 ANLLTENS--VFDRVFFTNSGAEANEGAFKLARKWGKLNKNSAGEDRFEIITFNHSFHGR 141 A +L + + + V+F + G+EA E A KLAR++ R II S+HG Sbjct: 79 AAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQY---YVEVGQPQRRHIIARRQSYHGN 135 Query: 142 TIATMSASGKPGWDTMFAPQV-------PGFPKAEL----NDLASVERLISK-------- 182 T+ ++ G MF P + P + +D+ V+RL + Sbjct: 136 TLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDVQYVQRLADELEQKILSL 195 Query: 183 ---KTVAVMLEPVQGE-GGVIPATREFMQGLRRLTKNANLLLIVDEVQTGVGRTGELFGY 238 + +A + E V G G +P ++ + +R + +LLI+DEV +G+GRTG LF Sbjct: 196 GADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVMSGMGRTGYLFAC 255 Query: 239 QLSGIEPDIMTLGKGIGGGV-PLAALLAREEI--ACFEAG---EQGGTYNGNPLMTAVGA 292 + G+ PDI+ + KG+G G P+ A++ + I A + G TY G+ A Sbjct: 256 EEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQHGHTYIGHATACAAAV 315 Query: 293 AVIKELLKPDFLPAVKATGAYLSAELIKLSDKHGFEGE-RGEGLLRALKL 341 AV K + + L V+ G L +EL + G+ RG GL ++L Sbjct: 316 AVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLFVGVEL 365 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 127 bits (318), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 101/354 (28%), Positives = 158/354 (44%), Gaps = 37/354 (10%) Query: 18 PEIVFTEGSGMWMTDHTGKRYLDYLQGWAVNALGHAPQCISDALVAQGKKLINPSPA-FY 76 P I T G+W+ D G+RYLD + W VN GH I+ ALV Q +L + A F Sbjct: 31 PPIAITRAHGLWLHDSDGRRYLDAISSWWVNLFGHTNTRINAALVEQLGQLEHAMLAGFT 90 Query: 77 NAPSIELANLLTE--NSVFDRVFFTNSGAEANEGAFKLA-RKWGKLNKNSAGEDRFEIIT 133 + P + L+ L F+ + GA A E A K++ W +N D+ E I Sbjct: 91 HEPVVALSEQLAALTGQALGHCFYASDGASAVEIALKMSVHAW----RNGGQPDKNEFIC 146 Query: 134 FNHSFHGRTIATMSASGKP----GWDTMFAP-QVPGFPKAE------------LNDLASV 176 +HG T+ + + P ++++ P V P A L V Sbjct: 147 IAGGYHGETVGALGVTDTPLFRSAYESLLRPAHVAASPDARQAAPGESAEEVAARALDDV 206 Query: 177 ERLISKKT---VAVMLEP-VQGEGGVIPATREFMQGLRRLTKNANLLLIVDEVQTGVGRT 232 ER++ ++ A+++EP VQ G+ +++GLR L LI DE+ G GRT Sbjct: 207 ERILQQRQGRIAALIVEPLVQCAAGMAMHDPGYVRGLRSLCDRYGAHLIADEIAVGCGRT 266 Query: 233 GELFGYQLSGIEPDIMTLGKGIGGG-VPLAALLAREEI--ACFEAGEQGG-----TYNGN 284 G F + +G+ PD++ L KGI GG +PL+ +++ E I A ++ + G +Y GN Sbjct: 267 GSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMSSEAIYQAFYDDDVRRGFLHSHSYTGN 326 Query: 285 PLMTAVGAAVIKELLKPDFLPAVKATGAYLSAELIKLSDKHGFEGERGEGLLRA 338 PL A + + L +A L+A L L+ R G++ A Sbjct: 327 PLACRAALATLAIFAEDAVLERNRAFAPVLAAALQPLAQDPRVRHVRQRGMIWA 380 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,245,011 Number of extensions: 54041 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 11 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)