BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0059.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 632 0.0 gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 127 2e-30 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 61 1e-10 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 60 3e-10 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 60 4e-10 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 632 bits (1629), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/392 (81%), Positives = 345/392 (88%) Query: 6 NHPRFLIFFICMLASAGQLAIDIYVPALPTMAQYFQTSAQAIQSSVTIYLMAYAFGQLFF 65 NHPRFL+F IC ASAGQLAID+YVPALP MA + TSAQAIQSSVT YL+AYAFGQL F Sbjct: 17 NHPRFLLFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVF 76 Query: 66 GPLADAYGRKTMLILGLAMFTAGCLLTLAANNLETFILARCLQGFGIAATNLLAKAIITD 125 GPLADAYGRK +L LGL +F+ GC+L+LAA NLETF+ AR LQGFGIAATNLLAKAIITD Sbjct: 77 GPLADAYGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITD 136 Query: 126 SFSGIALMHAFTYMAIAWGLAPILAPVIGAHLQTGFGWRSCLTFLLIYSFVMWAVLSRYK 185 SF+G AL+HA+TYMAIAWGLAPI+APVIGAHLQ FGWRSCL FLL+YS MWAVL RY+ Sbjct: 137 SFAGQALVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMWAVLWRYR 196 Query: 186 ETLKQPVHLHPKTLLRNAGMVLRSPVFQSCFLAQGLCYSILLVFNIVGPFMVQETLHKPP 245 ETL QPV LHP TL RNA MVL SPVFQSCFLAQGLCYSILLVFNIVGPFMVQ TLH+PP Sbjct: 197 ETLAQPVRLHPATLARNARMVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLHQPP 256 Query: 246 TYFGYLALAIGLMYFLGGISNRIHHPRLPTPEQRLRIGARVMTGAAVVMLILALTVGLEV 305 T+FGYLALAIG+MYFLGGISNRI H RLPT EQRLR+GARVMTGAA VML+LAL+VGL V Sbjct: 257 TFFGYLALAIGMMYFLGGISNRITHRRLPTAEQRLRLGARVMTGAAFVMLLLALSVGLTV 316 Query: 306 WTLTLPVLFMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLVSSLMMGLAGFVPF 365 WTL PVL MAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLVS+ MMGLAGFV Sbjct: 317 WTLCAPVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLVSAAMMGLAGFVSI 376 Query: 366 HSLTPMAIFFVIVGLTVISMVGKLLHHLXTGA 397 HSLTP+A FF +VGLTVI MVGKLLHHL A Sbjct: 377 HSLTPLAGFFCVVGLTVIVMVGKLLHHLQVPA 408 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 127 bits (318), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 87/263 (33%), Positives = 139/263 (52%), Gaps = 10/263 (3%) Query: 3 TTANHPRFLIFFICMLASAGQLAIDIYVPALPTMAQYFQTSAQAIQSSVTIYLMAYAFGQ 62 +T P +L + L + G LAID+Y+P+ +AQ +Q ++ +L+ A GQ Sbjct: 9 STGKFPAWLAV-LAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIGVAVGQ 67 Query: 63 LFFGPLADAYGRKTMLILGLAMFTAGCLLTLAANNLETFILARCLQGFGIAATNLLAKAI 122 +F+GPL+D +GRK L G+A++ LL + A N+ET I+ R QG G +A ++ +A+ Sbjct: 68 MFYGPLSDRFGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMVIPRAM 127 Query: 123 ITDSFSGIALMHAFTYMAIAWGLAPILAPVIGAHLQTGFGWRSCLTFLLIYSFVMWAVLS 182 + D AF+ + + +GLAPILAP IG + WR L ++ + Sbjct: 128 VRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGLLCILGTR 187 Query: 183 RY-KETLK----QPVHLHPKTLLRNAGMVLRSPVFQSCFLAQGLCYSILLVFNIVGPFMV 237 +Y ET+ +P+HL +TL + G+ LR F S L GL + + + GPF+V Sbjct: 188 KYLTETVDVSRAEPLHLG-RTLRQYWGL-LRHRQFMSYVLCGGLVQAGMFAYIAGGPFVV 245 Query: 238 QETLHKPPTYFGYL--ALAIGLM 258 E P YFG++ + AIGL+ Sbjct: 246 IELHGVKPQYFGFVFASNAIGLI 268 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 40/159 (25%), Positives = 75/159 (47%) Query: 32 ALPTMAQYFQTSAQAIQSSVTIYLMAYAFGQLFFGPLADAYGRKTMLILGLAMFTAGCLL 91 +LP +A+ ++ ++T Y+ A G +AD G + + + +F G +L Sbjct: 9 SLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMIVFMTGSIL 68 Query: 92 TLAANNLETFILARCLQGFGIAATNLLAKAIITDSFSGIALMHAFTYMAIAWGLAPILAP 151 + +L TF+ AR LQG G A + + II S L+ A +Y+ + L P++ P Sbjct: 69 CAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPALLGPVVGP 128 Query: 152 VIGAHLQTGFGWRSCLTFLLIYSFVMWAVLSRYKETLKQ 190 ++G + T F WR + + F+ + R+ + K+ Sbjct: 129 LLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKE 167 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 60.5 bits (145), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 67/257 (26%), Positives = 102/257 (39%), Gaps = 19/257 (7%) Query: 24 LAIDIYVPALPTMAQYFQTSAQAIQSSVTIYLMAYAFGQLFFGPLADAYGRKTMLILGLA 83 L I ALP MAQ F + +T Y++ A G +AD +G +T+ + +A Sbjct: 45 LDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIA 104 Query: 84 MFTAGCLLTLAANNLETFILARCLQGFGIAATNLLAKAIITDSFSGIALMHAFTYMAIAW 143 +FT +L + N+ F AR LQG G A + + I+ S LM A + Sbjct: 105 IFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPA 164 Query: 144 GLAPILAPVIGAHLQTGFGWRSCLTFLLIYSFVMWAVLSRYKETLKQPVHLHPKTLLRNA 203 +AP++ P IG + T W FL+ F + A + ++RN Sbjct: 165 IVAPVVGPSIGGFITTYASWH--WIFLMNVPFGLAAFTAVMM-------------VVRNE 209 Query: 204 GMVLRSPVFQSCFLAQGLCYSILLVFNIVGPFMVQETLHKPPTYFGYLALAIGLMYFLGG 263 V + P+ FL G + LL + M G++AL + L Y Sbjct: 210 HGVSKRPLDLVGFLLSGAALTSLLYGTEIASHMESNI----KVALGFVALGVVLGYGAWR 265 Query: 264 ISNRIHHPRLPTPEQRL 280 RI HP L R+ Sbjct: 266 HMQRIEHPLLDFSNLRI 282 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 59.7 bits (143), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 56/185 (30%), Positives = 84/185 (45%), Gaps = 19/185 (10%) Query: 33 LPTMAQYFQTSAQAIQSSVTIYLMAYAFGQLFFGPLADAYGRKTMLILGLAMFTAGCLLT 92 LPT+A+ S I+ V+ Y++ +A L G AD YGR+ +L+ G+A+F L Sbjct: 59 LPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRRVLLGGIALFALTSLAC 118 Query: 93 LAANNLETFILARCLQGFGIAATNLLAKAIITDSFSGIALMHAFTYMAIAW-------GL 145 A + + AR LQG G A A A+I +F H AW GL Sbjct: 119 ALAPSAQALYGARALQGVGAAFLLAPALAVIGHAF------HDEDQRERAWALWGGIMGL 172 Query: 146 APILAPVIGAHLQTGFGWRSC----LTFLLIYSFVMWAVLSRYKETLKQPVHLHPKTLLR 201 +LAP+IG + T GWR L L+ + +W + + L +P L P +L Sbjct: 173 TMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPLPRP--LDPLGILS 230 Query: 202 NAGMV 206 ++ V Sbjct: 231 SSSAV 235 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.142 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,287,436 Number of extensions: 53611 Number of successful extensions: 330 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 330 Number of HSP's successfully gapped: 12 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)