BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0044.p (844 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314042|ref|YP_003778134.1| signal transduction protein con... 346 5e-96 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 321 2e-88 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 310 2e-85 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 301 2e-82 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 299 5e-82 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 346 bits (887), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 198/518 (38%), Positives = 295/518 (56%), Gaps = 18/518 (3%) Query: 203 FPPTQAQSEKERYLSADRSAFPLQFQNQWLTRNGQ------IRTLLCSLSILENDYF--- 253 + T+A + R+L R + P F+ W+ Q +R S L ND++ Sbjct: 429 YSSTEAVGKSIRFLEG-RDSDPEGFETVWIAMREQREHNVVLRNYHKDGSELWNDFYIAP 487 Query: 254 -------KTVFILSGIDITQWQEAEERVYRLSHFDQISGLPNRAVLRDRLEQAALRHDPE 306 T F+ + DIT + E + + D ++GL NR +LR LEQA + + Sbjct: 488 VRDESGEVTHFVAALYDITSMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQ 547 Query: 307 AGSVVHILVELNGLARLRDALGAQAGVALAIAIAGRLQAWKPPGPTTIAQYADRVFAVLI 366 ++ + V+L+ + D LG +AG L +AGRL+A T D +L Sbjct: 548 KQALWVVFVDLDRFKFVNDTLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILP 607 Query: 367 EHVKREDIDAVVQHLLRTLEAPYSIPGQEEIHLQPKIGIALYPDDTRSAESLFHFSEVAL 426 E + +Q ++ + AP ++ G + + +G+A YP+D E+L +++A+ Sbjct: 608 EQDELTLDTRSLQRIMDAVAAPVTLGGHT-LFMTCSVGVACYPNDGTDGETLIKHADIAM 666 Query: 427 HRAQGNAYEHTHFYTRSFNQEIAARHVLANQLRQAIQREELVLHYQPQVSLKNGKITGFE 486 +R + + FYT + N++ R + LR A+ E +LHYQP+V L +G++ G E Sbjct: 667 YRGKQTGRNNFQFYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSE 726 Query: 487 ALVRWRHPERGLLLPAEFIALAEESETILVLGEWVLRAACRQCKQWQDQGLPPVTIAVNL 546 AL+RW+HP+ G++ PA FI LAEE+ IL +G WVLRAAC QC+QWQ G ++++VNL Sbjct: 727 ALIRWQHPQLGMVSPARFIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNL 786 Query: 547 SAMQFNEQNLRVLVGHILEESRLDARHLELELTESVSMDDPEKSIAIMVHLRKIGVTLSI 606 S QF +++L + +LE++ L LELELTES+ M D E +I I+ L+K+GV LSI Sbjct: 787 SVGQFLQKDLVSSIADVLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSI 846 Query: 607 DDFGTGYSNLGYLKRFPVGRLKIDKSFVRDLVNDPHDLAICRSVIALAKSLRLEVIAEGV 666 DDFGTGYS+L YLKRFP+ LKID+SFVRD+ D D AI S+I+LA+ L+L VIAEGV Sbjct: 847 DDFGTGYSSLAYLKRFPIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGV 906 Query: 667 ETINQLKLLHAEGCDKIQGYYFSAAMSAEECTSFLARD 704 ET QL L GCD+IQGY FS + A++ L +D Sbjct: 907 ETDEQLDYLRHYGCDEIQGYLFSPPVPADKFEQILRQD 944 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 321 bits (822), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 180/436 (41%), Positives = 265/436 (60%), Gaps = 7/436 (1%) Query: 269 EAEERVYRLSHFDQISGLPNRAVLRDRLEQAALRHDPEAGSVVHI-LVELNGLARLRDAL 327 EAE R ++ +H D ++GLPNR +L + L Q ALR + G++ + V+L+ + D+L Sbjct: 259 EAEMR-HQATH-DALTGLPNRNMLHEHL-QRALRVARQKGALTAVAFVDLDQFKFINDSL 315 Query: 328 GAQAGVALAIAIAGRLQAWKPPGPTTIAQYADRVFAVLIEHVKRE-DIDAVVQHLLRTLE 386 G Q G L +A RLQA Q D VL + E I V+Q +L + Sbjct: 316 GHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRILAAVA 375 Query: 387 APYSIPGQEEIHLQPKIGIALYPDDTRSAESLFHFSEVALHRAQGNAYEHTHFYTRSFNQ 446 P+ G E + IG++ YP D + ESL ++ A++RA+ + F+ + Sbjct: 376 RPWQ-AGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMDS 434 Query: 447 EIAARHVLANQLRQAIQREELVLHYQPQVSLKNGKITGFEALVRWRHPERGLLLPAEFIA 506 +++ R + LR+A+ ++E L+YQP++SL++G++ G EAL+RW P++G++ P FI Sbjct: 435 QVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIP 494 Query: 507 LAEESETILVLGEWVLRAACRQCKQWQDQGLPPVTIAVNLSAMQFNEQNLRVLVGHILEE 566 AEE+ I+ +GEWVLR ACRQ + WQ GLPP+ +AVNLS Q N Q+L V +L + Sbjct: 495 FAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLN-QSLPDFVAGVLAQ 553 Query: 567 SRLDARHLELELTESVSMDDPEKSIAIMVHLRKIGVTLSIDDFGTGYSNLGYLKRFPVGR 626 S L A LELE+TE+V M D EKS+A + L+++G+ +S+DDFGTGYS+L YL+RFPV Sbjct: 554 SGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDA 613 Query: 627 LKIDKSFVRDLVNDPHDLAICRSVIALAKSLRLEVIAEGVETINQLKLLHAEGCDKIQGY 686 LKIDKSFVRD+V D AI +++I+LA L L VIAEGVE Q L CD++QGY Sbjct: 614 LKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGY 673 Query: 687 YFSAAMSAEECTSFLA 702 YF M T++++ Sbjct: 674 YFGKPMEVAVFTAWMS 689 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 310 bits (795), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 202/589 (34%), Positives = 334/589 (56%), Gaps = 22/589 (3%) Query: 130 RALQESHQRAIRRRTEEILSAESKLLSAVFDSAGAVGVMLDAQGRILRFNQAGEKATGHA 189 RA E RA+ + E L ++++L+ + +S + +D G I +N+ E+ G++ Sbjct: 104 RADTEQRVRALDQ-IEAKLEQQAQILNQMHESV----ITMDPAGFITSWNRGAEQLFGYS 158 Query: 190 SDDIQGLCFWDIFFPPTQAQSEKERYLSADRSAFPLQFQNQWLTRNGQIRTLLCSLSILE 249 + + G +F + + + L + ++ + + ++G++ SLS++ Sbjct: 159 AMEAIGRNV--LFLYENEDEEDSLHDLFLEHGGREMEVRRR--KKSGEVFWASLSLSVMR 214 Query: 250 NDYFKTVFILSGI-DITQWQEAEERVYRLSHFDQISGLPNRAVLRDRLEQA--ALRHDPE 306 + + ++ + DIT+ + AE+ ++ L+++D ++GLPNR +L ++QA RH+ Sbjct: 215 DQNGHPIGLIGYLNDITERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENL 274 Query: 307 AGSVVHILVELNGLARLRDALGAQAGVALAIAIAGRLQ-AWKPPGPTTIAQYADRVFAVL 365 G V + ++LN + D LG AG L + +A RL+ A + +A+ FAV Sbjct: 275 HGCV--MFIDLNRFKPINDTLGHVAGDMLLVEVAMRLRRALREQ--DVVARLGADEFAVA 330 Query: 366 IEHVKREDIDAVVQHLLRTLEAPYSIPGQEEIHLQPKIGIALYPDDTRSAESLFHFSEVA 425 + + ++ V Q LL + P+ I G E + + IG++LYP++ E+L +++A Sbjct: 331 LFGIDKDSPAYVAQKLLALFDDPFFIDGHE-LRVGASIGVSLYPENATDTETLLRLADIA 389 Query: 426 LHRA-QGNAYEHTHF--YTRSFNQEIAARHVLANQLRQAIQREELVLHYQPQVSLKNGKI 482 ++RA QG + Y+ N+ + L A + EL+L+YQP+V ++ I Sbjct: 390 MYRAKQGGENNEGGYACYSEEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAI 449 Query: 483 TGFEALVRWRHPERGLLLPAEFIALAEESETILVLGEWVLRAACRQCKQWQDQGLPPVTI 542 TG EALVRW+HPERGLLLP EFI +AEE+ I+ L +WVL A CRQ + W+D GLPP+ I Sbjct: 450 TGAEALVRWQHPERGLLLPGEFIPIAEETGLIVQLSDWVLDAVCRQARAWKDAGLPPIRI 509 Query: 543 AVNLSAMQFNEQNLRVLVGHILEESRLDARHLELELTESVSMDDPEKSIAIMVHLRKIGV 602 A+N++A +F ++L VG LE L LELE+TES+ M ++ I+IM + +G+ Sbjct: 510 ALNVTAREFT-RSLPDRVGAALERHMLSGEWLELEITESMLMHSTDRVISIMEKICGLGI 568 Query: 603 TLSIDDFGTGYSNLGYLKRFPVGRLKIDKSFVRDLVNDPHDLAICRSVIALAKSLRLEVI 662 T+S+DDFGTGYS+L YLKRFP+ LKID+SF + +D +D AI ++I +AK LR +VI Sbjct: 569 TVSLDDFGTGYSSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVI 628 Query: 663 AEGVETINQLKLLHAEGCDKIQGYYFSAAMSAEECTSFLARDVSLPLDA 711 AEGVE Q L GC+++QGY FS + A + + L L L++ Sbjct: 629 AEGVEDEKQFNFLREAGCNELQGYIFSRPVPAPDFHAMLLEGRKLALES 677 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 301 bits (770), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 169/436 (38%), Positives = 254/436 (58%), Gaps = 3/436 (0%) Query: 268 QEAEERVYRLSHFDQISGLPNRAVLRDRLEQAALRHDPEAGSVVHILVELNGLARLRDAL 327 + A+ER+ L+H+D+++GLPNR + + A + G V IL++L+ + D Sbjct: 660 RRADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKNINDTF 719 Query: 328 GAQAGVALAIAIAGRLQAWKPPGPTTIAQYADRVFAVLIEHVKRE-DIDAVVQHLLRTLE 386 G + G A+ IA R + +A+ F VL++ + + Q LL Sbjct: 720 GHETGEAVLQEIAQRFRN-SLRELDILARVGGDEFIVLVDDFDDPVQLGEIAQKLLTEAR 778 Query: 387 APYSIPGQEEIHLQPKIGIALYPDDTRSAESLFHFSEVALHRAQGNAYEHTHFYTRSFNQ 446 P+ I GQE + L IGIA YP D +A++L +++A++RA+ + + F++ N Sbjct: 779 KPFIIDGQEAM-LSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEINT 837 Query: 447 EIAARHVLANQLRQAIQREELVLHYQPQVSLKNGKITGFEALVRWRHPERGLLLPAEFIA 506 R L +LR+AI+R E V+HYQP++ L G+I G EALVRW+HP RGLL P EFI Sbjct: 838 NTVERIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFIT 897 Query: 507 LAEESETILVLGEWVLRAACRQCKQWQDQGLPPVTIAVNLSAMQFNEQNLRVLVGHILEE 566 LAEE+ I +G VL ACR + Q + I++NL+ QF ++ L + +++ Sbjct: 898 LAEETRLIDQIGLLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIKEVVDR 957 Query: 567 SRLDARHLELELTESVSMDDPEKSIAIMVHLRKIGVTLSIDDFGTGYSNLGYLKRFPVGR 626 LE E+TES+ M + E++IAIM +R G T+SIDDFGTGYS+L YLKRFPV Sbjct: 958 HGTPPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNT 1017 Query: 627 LKIDKSFVRDLVNDPHDLAICRSVIALAKSLRLEVIAEGVETINQLKLLHAEGCDKIQGY 686 LKIDKSF+ D+ ++P+ AI +++IA++ +L L+V+ EGVET QL+ L G D+ QGY Sbjct: 1018 LKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGY 1077 Query: 687 YFSAAMSAEECTSFLA 702 YFS + + LA Sbjct: 1078 YFSKPVPYAQFVQLLA 1093 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 299 bits (766), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 198/576 (34%), Positives = 306/576 (53%), Gaps = 43/576 (7%) Query: 148 LSAESKLLSAVFDSAGAVGVMLDAQGRILRFNQAGEKATGHASDDIQGLCFWDIFFPPTQ 207 + AE ++ + FDS + ++ DA +ILR N+A + TG+++++I G Sbjct: 470 IEAELRISAVAFDSREGM-LITDANSKILRVNKAFTEITGYSAEEIVG------------ 516 Query: 208 AQSEKERYLSADRSAFPLQFQ---------NQWLT------RNGQIRTLLCSLSILEN-D 251 + L +DR P FQ +W +NG++ ++S ++N D Sbjct: 517 ---KHPNLLRSDRHG-PAFFQEMRESIRRFGKWQGEIWDRRKNGEVYPEWLTISAVKNKD 572 Query: 252 YFKTVFILSGIDITQWQEAEERVYRLSHFDQISGLPNRAVLRDRLEQAALRHDPEAGSVV 311 T ++ + DI+ + AEER+ L+ FD ++ LPNR +L DRL+QA Sbjct: 573 QQVTHYVSTHHDISDRKLAEERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAA 632 Query: 312 HILVELNGLARLRDALGAQAGVALAIAIAGRLQAWKPPGPTTIAQYADRVFAVLIEHVKR 371 + ++L+ L D +G + G L +A RL A T+A+ F V++E + Sbjct: 633 LLFIDLDHFKTLNDTIGHEKGDTLLKQVAQRLLA-NVRENDTVARVGGDEFVVVLEGLNE 691 Query: 372 EDIDAVVQ------HLLRTLEAPYSIPGQEEIHLQPKIGIALYPDDTRSAESLFHFSEVA 425 +A Q +L L + Y + E IG ++ S + L +++A Sbjct: 692 LQQEAATQTRAIGEKILTALGSTYQLD-DVEYRTTASIGATVFLGRAASVDELMKQADLA 750 Query: 426 LHRAQGNAYEHTHFYTRSFNQEIAARHVLANQLRQAIQREELVLHYQPQVSLKNGKITGF 485 +++A+ F+ + + R L LR+AI +L+LHYQPQV ++ G++TG Sbjct: 751 MYKAKETGRNALRFFDPAMQTAVLERATLEAGLRKAIDGNQLLLHYQPQV-VEGGRVTGA 809 Query: 486 EALVRWRHPERGLLLPAEFIALAEESETILVLGEWVLRAACRQCKQWQDQ-GLPPVTIAV 544 E LVRW+HP+RG++ P +FI LAEE+ IL LG WVL ACRQ W Q + ++IAV Sbjct: 810 EVLVRWQHPQRGMVPPGDFIPLAEETGLILSLGNWVLETACRQLATWAAQPQMADLSIAV 869 Query: 545 NLSAMQFNEQNLRVLVGHILEESRLDARHLELELTESVSMDDPEKSIAIMVHLRKIGVTL 604 N+SA Q E + V +E + + R L+LELTES+ +D+ E I M L+ GV Sbjct: 870 NVSAPQLREPDFVQTVLDTIERTGANPRRLKLELTESLLVDNVEDIIQKMFALKAYGVEF 929 Query: 605 SIDDFGTGYSNLGYLKRFPVGRLKIDKSFVRDLVNDPHDLAICRSVIALAKSLRLEVIAE 664 S+DDFGTGYS+L YLKR P+ +LKID+SFVRD++ DP+D +I ++++ LA+SL L VIAE Sbjct: 930 SLDDFGTGYSSLSYLKRLPLNQLKIDQSFVRDVLIDPNDASIAKTIVNLAQSLGLGVIAE 989 Query: 665 GVETINQLKLLHAEGCDKIQGYYFSAAMSAEECTSF 700 GVET Q L GC QGY+F ++ E F Sbjct: 990 GVETDAQRSFLADAGCHAYQGYFFCRPVTVEAFEKF 1025 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,500,222 Number of extensions: 101489 Number of successful extensions: 553 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 541 Number of HSP's successfully gapped: 25 Length of query: 844 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 751 Effective length of database: 1,179,515 Effective search space: 885815765 Effective search space used: 885815765 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)