BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0043.p (443 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 553 e-159 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 134 1e-32 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 130 3e-31 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 114 1e-26 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 108 1e-24 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 553 bits (1426), Expect = e-159, Method: Compositional matrix adjust. Identities = 272/429 (63%), Positives = 324/429 (75%), Gaps = 2/429 (0%) Query: 5 FETFESSPLSSADRVAD--VAPVNIAQRFEYLIANTPAIIYSSVPSGDFKMTFVSENASR 62 FE + PL +A A A + R+ ++I NTPAIIYSSVPSGDF++TFVSENASR Sbjct: 7 FEVSQVCPLPAAKEEAPDFCALIKANARYRHIIGNTPAIIYSSVPSGDFRLTFVSENASR 66 Query: 63 VLGYDPKEMVADPNFWFDHIHPDDAPDIFSSLAMLFVEGERAYEYRFLTKDKRYLWMHDS 122 VLGY+P EM+ DPNFWF+HIHPDD IFSSLAMLFVE ++ YEYRF T + YLWMHD+ Sbjct: 67 VLGYEPCEMLDDPNFWFNHIHPDDTAQIFSSLAMLFVEDQKIYEYRFRTSNGSYLWMHDT 126 Query: 123 LRLIRDENNNPVEVIGSLTNITERKLMEEALQRTGEEQRSLINKLKEAQEQLLQSEKMAS 182 LRLIRD + P EVIG +T+IT RK ME+ LQR EEQR LI +LK AQ QL+QSEK+AS Sbjct: 127 LRLIRDPDGTPQEVIGLMTDITGRKEMEDTLQRQAEEQRKLIEQLKMAQAQLMQSEKLAS 186 Query: 183 IGQLAAGVAHEINNPVGFVSSNMKSLQTYVDQLLEVIVRQDALLSDDSLGEDLRQQARQL 242 IGQLAAGVAHEINNP+GFVS+N+ +L YV LLE I L ++Q R Sbjct: 187 IGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCLQGGALPAAMAQEQLRSF 246 Query: 243 SASADLAYLKGDVVELITESLEGLKRVKDIVQSLKDFSHVGESDWQMVDIRKGLESTITI 302 A ADLA+L DV EL+ ES EGL+RV++IVQSLKDFS +GES+WQM D+ +GL+ST+ I Sbjct: 247 EARADLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSRIGESNWQMTDLHRGLDSTLNI 306 Query: 303 ANNELKYKTVIERQYGELPLIKGLASQLNQVFMNLLVNAAHSFAERGTIRIRTECKEDWV 362 ANNELKYK I +QYGELPL+K LAS+LNQVFMNLLVNAA + +ERGT+ I T D V Sbjct: 307 ANNELKYKVSIVKQYGELPLVKCLASELNQVFMNLLVNAAQAISERGTVTICTARAGDKV 366 Query: 363 YIQVEDTGCGIPPENLSRIFEPFFTTKPIGSGTGLGLSLSYGIVQKHGGRIDVQSEVGVG 422 + + DTG GI PENL+RIFEPFFTTKP+GSGTGLGLSLSYGIV+KHGG I V S G G Sbjct: 367 SVSISDTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCG 426 Query: 423 TCFTVCLPV 431 T FT+ LP+ Sbjct: 427 TTFTIELPI 435 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 134 bits (337), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 118/407 (28%), Positives = 183/407 (44%), Gaps = 82/407 (20%) Query: 56 VSENASRVLGYDPKEMVADPNFWFDHIHPDDAPDIFSSLAMLFVEGERAY--EYRFLTKD 113 V++ R+LG +EM+ D HPDD P + SL +E R++ E R+L D Sbjct: 596 VNQALCRMLGRSEEEMLG--MTLADVTHPDDVP-MNMSLFNRCIEDGRSFTLEKRYLRPD 652 Query: 114 KRYLWMHDSLRLIRDENNNPVEVIGSLTNITERKLMEEALQRTGE--EQRSL--INKLKE 169 W+ + I D P +I T+ITER+ EEAL+ + EQR + I + Sbjct: 653 GSTFWVSSDISRIVDGEGRPQAMIAVSTDITERRRAEEALRELNDTLEQRVVEEILERSR 712 Query: 170 AQEQLLQSEKMASIGQLAAGVAHEINNPVGFVSSNMKSLQTYVDQLLEVIVRQDALLSDD 229 A+ L Q +KM ++GQL GVAH+ NN + + N++ LQ SD Sbjct: 713 AEAALRQMQKMEAVGQLTGGVAHDFNNVLQIIGGNLQLLQ-----------------SDP 755 Query: 230 SLGEDLRQQARQLSASAD---------LAYLKGDVVELITESLEGLKRVKDIVQSLKDFS 280 L E RQ+ R + D LA+ + ++ + +LE +VQ++ D Sbjct: 756 RLDERSRQRVRIAIGAVDRGAKLSSQLLAFARRQPLQPRSINLE------RVVQNMDDL- 808 Query: 281 HVGESDWQMVDIRKGLESTITIANNELK--YKTVIERQYGELPLIKGLASQLNQVFMNLL 338 +R+ + I I+ + T+++ +Q+ + +N Sbjct: 809 -----------LRRAVGENIDISTRVAHDLWNTMLD------------PNQVENLIINTA 845 Query: 339 VNAAHSFAERG--TIRIRTECKED-------------WVYIQVEDTGCGIPPENLSRIFE 383 +NA + G T++I ED +V +Q+ D G G+ PE R FE Sbjct: 846 INARDAMPSGGRLTVQIDNVRLEDPVFLSQADLAPGEYVELQISDNGAGMSPEVRDRAFE 905 Query: 384 PFFTTKPIGSGTGLGLSLSYGIVQKHGGRIDVQSEVGVGTCFTVCLP 430 PFFTTK G GTGLGLS++YG V++ G I + SE G GT + P Sbjct: 906 PFFTTKLEGEGTGLGLSMAYGFVKQSKGHISIHSEQGKGTTIRIYFP 952 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 130 bits (326), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 102/381 (26%), Positives = 165/381 (43%), Gaps = 72/381 (18%) Query: 82 IHPDDAPDIFSSL-AMLFVEGERAYEYRFLTKDKRYLWMHDSLRLIRDENNNPVEVIGSL 140 +HPDD + + + L G A++YR +D RY W+ + R+ + P + G L Sbjct: 197 VHPDDRELLSARIREALARGGAYAHQYRTRRRDGRYYWLEANGRVEMTPDGIPSKFPGVL 256 Query: 141 TNITERKLMEEALQRTGEEQRSLINKLKE-----------AQEQLLQSEKMASIGQLAAG 189 +I R+ +E R R+L L++ A+E L Q++KM ++GQL G Sbjct: 257 IDIEGRRAVEAERDRALAALRTLNETLEQRVEERTAALLNAEEALRQAQKMEAVGQLTGG 316 Query: 190 VAHEINNPVGFVSSNMKSLQTYVDQLLEVIVRQDALLSDDSLGEDLRQQARQLSASADLA 249 +AH+ NN + +S ++ +L++V + Q + Sbjct: 317 LAHDFNNILAGISGSL--------ELMKVRLAQGRI------------------------ 344 Query: 250 YLKGDVVELITESLEGLKRVKDIVQSLKDFSHVGESDWQMVDIRKGLESTITIANNELKY 309 GD+ + + +KR + Q L FS D + D+ + I +EL Sbjct: 345 ---GDIERHLNGAQSAVKRAAALTQRLLAFSRRQTLDPKPADLNR-----IVAGMHELIG 396 Query: 310 KTVIERQYGELPLIKGL------ASQLNQVFMNLLVNAAHSFAERGTIRIRTECK----- 358 ++V E GL Q+ +NL +NA + G + I T + Sbjct: 397 RSVGPAIAVETIAAGGLWHTFVDIGQMENALLNLCINARDAMPHGGRLTIETANRWLDDI 456 Query: 359 ---------EDWVYIQVEDTGCGIPPENLSRIFEPFFTTKPIGSGTGLGLSLSYGIVQKH 409 +V + V DTG G+ PE ++R F+PFFTTKP G GTGLGLS+ YG + Sbjct: 457 GALQRGVPPGQYVSLSVSDTGAGMSPEVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQS 516 Query: 410 GGRIDVQSEVGVGTCFTVCLP 430 GG + + SE+G G + LP Sbjct: 517 GGTVRIYSELGQGAMVCIYLP 537 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 114 bits (286), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 100/350 (28%), Positives = 155/350 (44%), Gaps = 68/350 (19%) Query: 110 LTKDKRYLWMHDSLRLIRDENNNPVEVIGSLTNITERKLME-EALQRTGEEQ-----RSL 163 ++ D +W + L ++R+ + + N +R L E EA R E + Sbjct: 391 VSSDAPRVWTENELAIVREVSERACAAFERIRN--QRALAESEARLRAINESLEAEVSAR 448 Query: 164 INKLKEAQEQLLQSEKMASIGQLAAGVAHEINNPVGFVSSNMKSLQTYVDQLLEVIVRQD 223 +L +E L S+K+ +IGQL G+AH+ NN +G +S ++ ++ + Q Sbjct: 449 TRELMATEEALRHSQKIEAIGQLTGGLAHDFNNLLGGISGSLDLMRVRLVQ--------- 499 Query: 224 ALLSDDSLGEDLRQQARQLSASADLAYLKGDVVELITESLEGLKRVKDIVQSLKDFSHVG 283 GE+L L Y VEL S + R + Q L F+ Sbjct: 500 ------GRGEEL------------LRY-----VELAARSTQ---RAASLTQRLLAFARR- 532 Query: 284 ESDWQMVDIRKGLESTITIANNELKYKTVIERQYGELPLIKGL------ASQLNQVFMNL 337 Q + + + + EL +T+ E+ GL QL +NL Sbjct: 533 ----QTLAPKPTRVNALISGMEELIRRTIGPAIVLEVVGAGGLWPTFVDGPQLESALLNL 588 Query: 338 LVNAAHSFAERGTIRIRTECK---EDW-----------VYIQVEDTGCGIPPENLSRIFE 383 +NA + G + I T K + W V I V D+G G+PP+ L+R F+ Sbjct: 589 CINARDAMPGGGRLTIETANKWLDDRWAHERDLPPGQYVCISVTDSGVGMPPDVLARAFD 648 Query: 384 PFFTTKPIGSGTGLGLSLSYGIVQKHGGRIDVQSEVGVGTCFTVCLPVTQ 433 PFFTTKPIG+GTGLGLS+ YG ++ GG++ + SEVG GT ++ LP Q Sbjct: 649 PFFTTKPIGAGTGLGLSMVYGFARQSGGQVRLYSEVGQGTTVSIYLPRHQ 698 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 108 bits (269), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 85/292 (29%), Positives = 135/292 (46%), Gaps = 61/292 (20%) Query: 164 INKLKEAQEQLLQSEKMASIGQLAAGVAHEINNPVGFVSSNMKSLQTYVDQLLEVIVRQD 223 I++ ++A++ L Q+ KM ++GQL G+AH+ NN + ++ Y+D ++ + +R D Sbjct: 157 ISRRRDAEQALGQARKMEALGQLTGGIAHDFNNLLQVITG-------YLD-MINLALRSD 208 Query: 224 ALLSDDSLGEDLRQQARQLSASADLAYLKGDVVELITESLEGLKRVKDIVQSLKDFSHVG 283 DL + AR SAD ++T+ L R + + + + + Sbjct: 209 K--------PDLARVAR----SADSIRKASGKAAMLTQQLLAFARKQRLEGRTINLNSLT 256 Query: 284 ESDWQMVDIRKGLESTITIANNELKYKTVIERQYGELPLIKGL------ASQLNQVFMNL 337 E +V + L IT+ + L +GL +Q+ +N+ Sbjct: 257 EGFTDLVS--RTLGGDITV----------------QTELARGLWNCRLDPTQMEVALLNV 298 Query: 338 LVNAAHSFAERGTIRIRTECKED----------------WVYIQVEDTGCGIPPENLSRI 381 L+NA + RG +RIRT ED +V I V D G GI P+ L R+ Sbjct: 299 LINARDAMNGRGRVRIRT-ANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPDILPRV 357 Query: 382 FEPFFTTKPIGSGTGLGLSLSYGIVQKHGGRIDVQSEVGVGTCFTVCLPVTQ 433 +PFFTTK G GTGLGLS+ YG V++ GG + + S GT T+ P TQ Sbjct: 358 MDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPATQ 409 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,199,333 Number of extensions: 45418 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 28 Number of HSP's gapped: 315 Number of HSP's successfully gapped: 30 Length of query: 443 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 355 Effective length of database: 1,203,190 Effective search space: 427132450 Effective search space used: 427132450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)