BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0042.p (464 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313028|ref|YP_003777120.1| two component response regulato... 418 e-118 gi|300310565|ref|YP_003774657.1| response regulator containing a... 109 5e-25 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 73 6e-14 gi|300313980|ref|YP_003778072.1| two component response regulato... 72 1e-13 gi|300313916|ref|YP_003778008.1| two component response regulato... 71 2e-13 >gi|300313028|ref|YP_003777120.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 448 Score = 418 bits (1075), Expect = e-118, Method: Compositional matrix adjust. Identities = 203/370 (54%), Positives = 279/370 (75%) Query: 16 ASPPPVVLLVDDEASILSSLKRVLRPKGYTLITAESGADGLRLLEEHPVDLIVSDMRMPE 75 A PPP VLLVDDE SILS+LKR RP+GY LI A S L ++ VDL++SDMRMP Sbjct: 19 ALPPPCVLLVDDEQSILSALKRTFRPQGYRLIMATSAEAALEMMAASEVDLVISDMRMPG 78 Query: 76 MSGAQFLGKAKERFPEVMRILLTGYSEITATISAINDGGIYHYLQKPWDEQDLLLTIQRA 135 M+GAQFL K ++++P MRILLTGYSEI +TISAINDGGI HYL KPWD+++LLL + A Sbjct: 79 MNGAQFLAKVRQQWPSTMRILLTGYSEIGSTISAINDGGINHYLSKPWDDRELLLIVAHA 138 Query: 136 LEQQHLKKEAVRLNELVRKQNEELQTFNARLEKQVQARTEEIRQTVMFLENSQDEIKLNF 195 LE+Q L+K+ + L VR+QN +L FN +LE+QVQ+RTEE+RQTVMFLE++Q+E + +F Sbjct: 139 LEEQQLRKQKMALERKVREQNLQLADFNRQLERQVQSRTEELRQTVMFLESAQEEEQKSF 198 Query: 196 LTMLKVFSNMIELRSGMLGGQSDRVSALSRKLGKKLKLHDVQLQDLSIAGLLHAIGKIGL 255 L+ML+VFS++ ELR+G+ GQS RV+ +L ++ + +D +AG+LH IGK+ L Sbjct: 199 LSMLRVFSDISELRAGLATGQSARVARWCEQLALRMGMTTHHARDTMMAGMLHGIGKLEL 258 Query: 256 PDELIRKPLEKMSGEESRSFMAHPVKGHMVLTPVSAFDEVGHLILYQYERYDGRGTPEGK 315 PDEL+RKP+EKM+ +E + F+ HPV+G M+LTP+ + E G +I +Q+ERYDGRG PE Sbjct: 259 PDELVRKPIEKMNSDEVQRFLWHPVRGQMLLTPIPSLAESGEIIRHQHERYDGRGVPESL 318 Query: 316 SGEAIPLGSRILAVARDFEALRSGAIATLPLPLDKAVQLIKSQSGHRYDPEVVLNFIALV 375 SG+AIPLG+RILAV RDFE L +G I +L +KA+ ++++ +GHRYDP VV +FIA++ Sbjct: 319 SGDAIPLGARILAVVRDFEGLCNGGITSLNASPEKALSMLRAHAGHRYDPLVVESFIAMM 378 Query: 376 EEGDSAVAER 385 EE A+ ++ Sbjct: 379 EEQQMALEQQ 388 >gi|300310565|ref|YP_003774657.1| response regulator containing a CheY-like receiver domain and an HD-GYP domain [Herbaspirillum seropedicae SmR1] Length = 348 Score = 109 bits (272), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 104/365 (28%), Positives = 164/365 (44%), Gaps = 43/365 (11%) Query: 22 VLLVDDEASILSSLKRVLRPKGYTLI-TAESGADGLRLLEEHPVDLIVSDMRMPEMSGAQ 80 +L+VDD+ + LS +L T I T L +EH DLI+ D MP M G Sbjct: 6 ILIVDDDKTNLSLFSHMLGQVPQTRIDTCADARQALDWCQEHAPDLILLDYMMPGMDGLD 65 Query: 81 FLGK-----AKERFPEVMRILLTGYS-EITATISAINDGGIYHYLQKPWDEQDLLLTIQR 134 FL + +E P +M G TA + ND +L KP + +LL ++ Sbjct: 66 FLSRFRAMPGQEMTPVIMITADMGLQVRHTALQMSAND-----FLSKPVNRVELLARVKN 120 Query: 135 ALEQQHLKKEAVRLNELVRKQNEELQTFNARLEKQVQARTEEIRQTVMFLENSQDEIKLN 194 L A+R ++L Q ++ T + ++++QV+ D + L+ Sbjct: 121 ML--------AMRRSQLAMSQ--KIDTLSEQVDQQVR---------------HSDTLALD 155 Query: 195 FLTMLKVFSNMIELRSGMLGGQSDRVSALSRKLGKKLKLHDVQLQDLSIAGLLHAIGKIG 254 + +L + R G R++ +R + ++ L Q + L A +H IGK+G Sbjct: 156 SVDLLARAAG---YRDPETGEHLTRMANYARIIAARMGLDSQQQELLLNAAPMHDIGKMG 212 Query: 255 LPDELIRKPLEKMSGEESRSFMAHPVKGH--MVLTPVSAFDEVGHLILYQYERYDGRGTP 312 +PD ++ KP ++ EE + H G +V +P + L +E++DG G P Sbjct: 213 IPDHILLKP-GRLDEEELKVMRMHARIGADILVASPSPLLQTASQIALTHHEKWDGSGYP 271 Query: 313 EGKSGEAIPLGSRILAVARDFEALRSGAIATLPLPLDKAVQLIKSQSGHRYDPEVVLNFI 372 G GE IPL RI+AVA F+AL S L++A Q ++ QSG +DP V F Sbjct: 272 NGLKGEQIPLYGRIVAVADVFDALTSSRPYKPGWSLERASQYLREQSGAHFDPRCVEAFF 331 Query: 373 ALVEE 377 A EE Sbjct: 332 AGWEE 336 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 72.8 bits (177), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 50/154 (32%), Positives = 77/154 (50%), Gaps = 3/154 (1%) Query: 220 VSALSRKLGKKLKLHDVQLQDLSIAGLLHAIGKIGLPDELIRKPLEKMSGEESRSFMAHP 279 V AL LG+ L L + ++ + G LH +GK +P EL+ KP K+S EE HP Sbjct: 166 VCALMVALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKP-GKLSDEEFSQIKKHP 224 Query: 280 VKGHMVLT-PVSAFDEVGHLILYQYERYDGRGTPEGKSGEAIPLGSRILAVARDFEALRS 338 G+ L+ + D + L+ +E+ DG G PEG AI L SR+ A+ ++AL S Sbjct: 225 ELGYKYLSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTS 284 Query: 339 GAIATLPLPLDKAVQLIKSQSGHRYDPEVVLNFI 372 +A+ + S +GH +D + L F+ Sbjct: 285 DRPYKRAWDPAEAISAMASWTGH-FDRAMFLAFV 317 >gi|300313980|ref|YP_003778072.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 199 Score = 72.0 bits (175), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 53/176 (30%), Positives = 85/176 (48%), Gaps = 8/176 (4%) Query: 200 KVFSNMIE-------LRSGMLGGQSDRVSALSRKLGKKLKLHDVQLQDLSIAGLLHAIGK 252 K++ +I+ L G D+ + L+ +G+ L L ++ L +A +H IGK Sbjct: 8 KIYQEVIDAMIAVHRLTDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMASQVHDIGK 67 Query: 253 IGLPDELIRKPLEKMSGEESRSFMAHPVKGHMVLTPVSAFDEVGHLILYQYERYDGRGTP 312 +P L+ KP ++S EE H G+ +L +S + ++ +E DG G P Sbjct: 68 ARIPLALLTKP-GRISVEEYDVLKTHVQFGYEILNKISFPFNLAEIVHCHHEYLDGSGYP 126 Query: 313 EGKSGEAIPLGSRILAVARDFEALRSGAIATLPLPLDKAVQLIKSQSGHRYDPEVV 368 G SG+ IPL +RIL VA E++ + L + A+ I+ QSG R D VV Sbjct: 127 RGISGDEIPLEARILTVADIVESMSADRPYRKSLGMSAALDEIQRQSGVRLDAVVV 182 >gi|300313916|ref|YP_003778008.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 448 Score = 70.9 bits (172), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 44/117 (37%), Positives = 65/117 (55%), Gaps = 2/117 (1%) Query: 22 VLLVDDEASILSSLKRVLRPKGYTLITAESGADGLRLLEEHPVDLIVSDMRMPEMSGAQF 81 +L+VDD+A+ SL + G+ ++ A S A GL+LL H VD + D+RMP+ G F Sbjct: 4 ILIVDDDAAFRGSLAETVSDLGHDVLEAGSTAAGLKLLAAHAVDAAIVDLRMPDEDGLAF 63 Query: 82 LGKAKERFPEVMRILLTGYSEITATISAINDGGIYHYLQKPWDEQDLLLTIQRALEQ 138 L +A + I+LT Y+ TI AI G + +L KP L+ T++RAL Q Sbjct: 64 LQRAPA-IAAIPCIMLTAYASGDNTIEAIRLGA-FDHLTKPVPRAALVETLERALRQ 118 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,274,274 Number of extensions: 51136 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 320 Number of HSP's successfully gapped: 18 Length of query: 464 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 375 Effective length of database: 1,198,455 Effective search space: 449420625 Effective search space used: 449420625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)