BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0041.p (272 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313028|ref|YP_003777120.1| two component response regulato... 125 4e-30 gi|300312425|ref|YP_003776517.1| nitrogen assimilation transcrip... 67 1e-12 gi|300313791|ref|YP_003777883.1| response regulator protein [Her... 59 4e-10 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 57 1e-09 >gi|300313028|ref|YP_003777120.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 448 Score = 125 bits (314), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 60/159 (37%), Positives = 106/159 (66%) Query: 11 PRTLLLVDDETNILSALKRLLRQDKYDIVTANNGQEALEALKSGPVDVIVTDQRMPGMTG 70 P +LLVDDE +ILSALKR R Y ++ A + + ALE + + VD++++D RMPGM G Sbjct: 22 PPCVLLVDDEQSILSALKRTFRPQGYRLIMATSAEAALEMMAASEVDLVISDMRMPGMNG 81 Query: 71 VEFLRLAKESYPDTVRIVLSGYTELQSVTDAVNEGAVYKFLTKPWDDGQLRGHVAEAFRR 130 +FL ++ +P T+RI+L+GY+E+ S A+N+G + +L+KPWDD +L VA A Sbjct: 82 AQFLAKVRQQWPSTMRILLTGYSEIGSTISAINDGGINHYLSKPWDDRELLLIVAHALEE 141 Query: 131 KEMADENLRLQQQLQMANLALEETNKELDKLLKVQEERI 169 +++ + + L+++++ NL L + N++L++ ++ + E + Sbjct: 142 QQLRKQKMALERKVREQNLQLADFNRQLERQVQSRTEEL 180 >gi|300312425|ref|YP_003776517.1| nitrogen assimilation transcription activator protein [Herbaspirillum seropedicae SmR1] Length = 500 Score = 67.4 bits (163), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 32/105 (30%), Positives = 65/105 (61%), Gaps = 1/105 (0%) Query: 12 RTLLLVDDETNILSALKRLLRQDKYDIVTANNGQEALEALKSGPVDVIVTDQRMPGMTGV 71 + + +VDD+ +I L++ L ++ + ++ ++A+ AL++G V+V+D RMPG +G+ Sbjct: 2 KPIWIVDDDESIRWVLEKALARENLATQSFSSARDAIAALQNGTPQVLVSDIRMPGASGL 61 Query: 72 EFLRLAKESYPDTVRIVLSGYTELQSVTDAVNEGAVYKFLTKPWD 116 E L+ K +P I+++ +++L S A GA +++L KP+D Sbjct: 62 ELLQTVKAKHPGIPVIIITAFSDLDSAVSAFQGGA-FEYLAKPFD 105 >gi|300313791|ref|YP_003777883.1| response regulator protein [Herbaspirillum seropedicae SmR1] Length = 206 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 40/121 (33%), Positives = 60/121 (49%), Gaps = 1/121 (0%) Query: 12 RTLLLVDDETNILSALKRLLRQDKYDIVTANNGQEALEALKSGPVDVIVTDQRMPGMTGV 71 RT+ +VDD+ + +AL RLL Y + E L+A ++ D +MPG G Sbjct: 3 RTVFVVDDDNMVRTALLRLLASAGYAAHGFASADEFLQARLPAVPCCLLLDMKMPGSDGF 62 Query: 72 EFLRLAKESYPDTVRIVLSGYTELQSVTDAVNEGAVYKFLTKPWDDGQLRGHVAEAFRRK 131 E +R + I +SGY + + A+ GA ++FLTKP D +L G V EA + Sbjct: 63 EVVRRLERRGDAIPVIFISGYGTIPTTVQAMKAGA-HEFLTKPIQDERLLGAVEEALQIA 121 Query: 132 E 132 E Sbjct: 122 E 122 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 38/108 (35%), Positives = 55/108 (50%), Gaps = 4/108 (3%) Query: 14 LLLVDDETNILSALKRLLRQDKYDIVTANNGQEALEALKSGPVDVIVTDQRMPGMTGVEF 73 LL+V+D + + LL + +G+EAL+AL+ DV+ TD +PGM+GVE Sbjct: 1166 LLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQALEEQGFDVLFTDVNLPGMSGVEL 1225 Query: 74 LRLAKESYPDTVRIVLSGYTELQSVTDAVNEGAVYKFLTKPWDDGQLR 121 + A +PD I SGY + A N G L KP+D QL+ Sbjct: 1226 AKAALGRFPDLAVIFASGYGTMV----ARNVGFAAHALPKPYDIDQLQ 1269 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.363 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 726,121 Number of extensions: 26557 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 208 Number of HSP's successfully gapped: 7 Length of query: 272 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 188 Effective length of database: 1,222,130 Effective search space: 229760440 Effective search space used: 229760440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)