BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0034.p (571 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 289 4e-79 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 101 2e-22 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 100 2e-22 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 100 3e-22 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 100 3e-22 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 289 bits (740), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 149/282 (52%), Positives = 195/282 (69%), Gaps = 8/282 (2%) Query: 287 TLKSEKARQDELIRKLADAQSQLLQSEKMASIGQLAAGVAHEINNPVGFVNSNLGTLQRY 346 TL+ + Q +LI +L AQ+QL+QSEK+ASIGQLAAGVAHEINNP+GFV++NL TL Y Sbjct: 156 TLQRQAEEQRKLIEQLKMAQAQLMQSEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNY 215 Query: 347 VDEIMALLSAYEQSE-----AEMPEEIRRALAGHKARIDLDFLREDIASVFSESQDGLQR 401 V ++ + ++ + A M +E R+ +AR DL FL ED+ + +ES +GLQR Sbjct: 216 VATLLEGIQLHQCLQGGALPAAMAQEQLRSF---EARADLAFLSEDVTELLAESNEGLQR 272 Query: 402 VIRIVQDLKTFSHVDEAEKQWANLEQGLDSTLNVVSHDLKYTAEIVKEYGGIPDIECLPF 461 V IVQ LK FS + E+ Q +L +GLDSTLN+ +++LKY IVK+YG +P ++CL Sbjct: 273 VRNIVQSLKDFSRIGESNWQMTDLHRGLDSTLNIANNELKYKVSIVKQYGELPLVKCLAS 332 Query: 462 QLNQVFLNLFVNAGQAIEGRGRIVLRTGHDEDQVWVEVEDSGKGIPEADLERIFEPFFTT 521 +LNQVF+NL VNA QAI RG + + T D+V V + D+G GI +L RIFEPFFTT Sbjct: 333 ELNQVFMNLLVNAAQAISERGTVTICTARAGDKVSVSISDTGHGIAPENLNRIFEPFFTT 392 Query: 522 KPVGTGTGLGLSLSYGIVAKHGGKITVRSEVGKGSVFRVSLP 563 KPVG+GTGLGLSLSYGIV KHGG I V S G G+ F + LP Sbjct: 393 KPVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTIELP 434 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 101 bits (251), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 87/322 (27%), Positives = 143/322 (44%), Gaps = 74/322 (22%) Query: 278 RVQLAVANQTLKSEKARQDELIRKLAD---------------AQSQLLQSEKMASIGQLA 322 R Q +A T +E+ R +E +R+L D A++ L Q +KM ++GQL Sbjct: 671 RPQAMIAVSTDITERRRAEEALRELNDTLEQRVVEEILERSRAEAALRQMQKMEAVGQLT 730 Query: 323 AGVAHEINNPVGFVNSNLGTLQRYVDEIMALLSAYEQSEAEMPEEIRRALAGHKARIDLD 382 GVAH+ NN + + NL LQ S+ + E R + RI + Sbjct: 731 GGVAHDFNNVLQIIGGNLQLLQ---------------SDPRLDERSR-----QRVRIAIG 770 Query: 383 FLREDIASVFSESQDGLQRVIRIVQDLKTFSHVDEAEKQWANLE---QGLDSTLN-VVSH 438 + R ++ L F+ + + NLE Q +D L V Sbjct: 771 ---------------AVDRGAKLSSQLLAFARRQPLQPRSINLERVVQNMDDLLRRAVGE 815 Query: 439 DLKYTAEIVKE-YGGIPDIECLPFQLNQVFLNLFVNAGQAIEGRGRIVLRTGHD--EDQV 495 ++ + + + + + D P Q+ + +N +NA A+ GR+ ++ + ED V Sbjct: 816 NIDISTRVAHDLWNTMLD----PNQVENLIINTAINARDAMPSGGRLTVQIDNVRLEDPV 871 Query: 496 WV-------------EVEDSGKGIPEADLERIFEPFFTTKPVGTGTGLGLSLSYGIVAKH 542 ++ ++ D+G G+ +R FEPFFTTK G GTGLGLS++YG V + Sbjct: 872 FLSQADLAPGEYVELQISDNGAGMSPEVRDRAFEPFFTTKLEGEGTGLGLSMAYGFVKQS 931 Query: 543 GGKITVRSEVGKGSVFRVSLPK 564 G I++ SE GKG+ R+ P+ Sbjct: 932 KGHISIHSEQGKGTTIRIYFPR 953 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 89/321 (27%), Positives = 139/321 (43%), Gaps = 62/321 (19%) Query: 269 RAVEQFQADRVQLAVA---------NQTLKSEKARQDELIRKLADAQSQLLQSEKMASIG 319 RA F+ R Q A+A N++L++E + + R+L + L S+K+ +IG Sbjct: 413 RACAAFERIRNQRALAESEARLRAINESLEAEVSAR---TRELMATEEALRHSQKIEAIG 469 Query: 320 QLAAGVAHEINNPVGFVNSNLGTLQRYVDEIMALLSAYEQSEAEMPEEIRRALAGHKARI 379 QL G+AH+ N+ LG + +D + R + G Sbjct: 470 QLTGGLAHDF-------NNLLGGISGSLDLMRV-----------------RLVQGRG--- 502 Query: 380 DLDFLREDIASVFSESQDGLQRVIRIVQDLKTFSHVDEAEKQWANLEQGLDSTLNVVSHD 439 E++ + QR + Q L F+ + + + ++ Sbjct: 503 ------EELLRYVELAARSTQRAASLTQRLLAFARRQTLAPKPTRVNALISGMEELIRRT 556 Query: 440 L--KYTAEIVKEYGGIPDIECLPFQLNQVFLNLFVNAGQAIEGRGRIVLRTG-------- 489 + E+V G P P QL LNL +NA A+ G GR+ + T Sbjct: 557 IGPAIVLEVVGAGGLWPTFVDGP-QLESALLNLCINARDAMPGGGRLTIETANKWLDDRW 615 Query: 490 -HDED-----QVWVEVEDSGKGIPEADLERIFEPFFTTKPVGTGTGLGLSLSYGIVAKHG 543 H+ D V + V DSG G+P L R F+PFFTTKP+G GTGLGLS+ YG + G Sbjct: 616 AHERDLPPGQYVCISVTDSGVGMPPDVLARAFDPFFTTKPIGAGTGLGLSMVYGFARQSG 675 Query: 544 GKITVRSEVGKGSVFRVSLPK 564 G++ + SEVG+G+ + LP+ Sbjct: 676 GQVRLYSEVGQGTTVSIYLPR 696 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 100 bits (250), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 89/316 (28%), Positives = 146/316 (46%), Gaps = 54/316 (17%) Query: 266 EMARAVEQFQADRV--QLAVANQTLKSEKARQDELIRKLADAQSQLLQSEKMASIGQLAA 323 E RAVE + DR L N+TL+ R +E L +A+ L Q++KM ++GQL Sbjct: 260 EGRRAVEA-ERDRALAALRTLNETLEQ---RVEERTAALLNAEEALRQAQKMEAVGQLTG 315 Query: 324 GVAHEINNPVGFVNSNLGTLQRYVDEIMALLSAYEQSEAEMPEEIRRALAGHKARIDLDF 383 G+AH+ NN + ++ +L E+M ++R A + RI Sbjct: 316 GLAHDFNNILAGISGSL--------ELM---------------KVRLA----QGRIG--- 345 Query: 384 LREDIASVFSESQDGLQRVIRIVQDLKTFSHVDEAEKQWANLEQGLDSTLNVVSHDLKYT 443 DI + +Q ++R + Q L FS + + A+L + + ++ + Sbjct: 346 ---DIERHLNGAQSAVKRAAALTQRLLAFSRRQTLDPKPADLNRIVAGMHELIGRSVGPA 402 Query: 444 AEI-VKEYGGIPDIECLPFQLNQVFLNLFVNAGQAIEGRGRIVLRTGH---DE------- 492 + GG+ Q+ LNL +NA A+ GR+ + T + D+ Sbjct: 403 IAVETIAAGGLWHTFVDIGQMENALLNLCINARDAMPHGGRLTIETANRWLDDIGALQRG 462 Query: 493 ----DQVWVEVEDSGKGIPEADLERIFEPFFTTKPVGTGTGLGLSLSYGIVAKHGGKITV 548 V + V D+G G+ + R F+PFFTTKP G GTGLGLS+ YG + GG + + Sbjct: 463 VPPGQYVSLSVSDTGAGMSPEVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGGTVRI 522 Query: 549 RSEVGKGSVFRVSLPK 564 SE+G+G++ + LP+ Sbjct: 523 YSELGQGAMVCIYLPR 538 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 100 bits (249), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 79/281 (28%), Positives = 120/281 (42%), Gaps = 47/281 (16%) Query: 299 IRKLADAQSQLLQSEKMASIGQLAAGVAHEINNPVGFVNSNLGTLQRYVDEIMALLSAYE 358 I + DA+ L Q+ KM ++GQL G+AH+ NN + + Y+D I L Sbjct: 157 ISRRRDAEQALGQARKMEALGQLTGGIAHDFNNLLQVITG-------YLDMINLAL---- 205 Query: 359 QSEAEMPEEIRRALAGHKARIDLDFLREDIASVFSESQDGLQRVIRIVQDLKTFSHVDEA 418 ++ P+ R A + R + + Q L F+ Sbjct: 206 --RSDKPDLARVARSADSIR------------------KASGKAAMLTQQLLAFARKQRL 245 Query: 419 EKQWANLEQGLDSTLNVVSHDLKYTAEIVKEYG-GIPDIECLPFQLNQVFLNLFVNAGQA 477 E + NL + ++VS L + E G+ + P Q+ LN+ +NA A Sbjct: 246 EGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLWNCRLDPTQMEVALLNVLINARDA 305 Query: 478 IEGRGRIVLRTGH----DEDQ-----------VWVEVEDSGKGIPEADLERIFEPFFTTK 522 + GRGR+ +RT + D D+ V + V D G GI L R+ +PFFTTK Sbjct: 306 MNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPDILPRVMDPFFTTK 365 Query: 523 PVGTGTGLGLSLSYGIVAKHGGKITVRSEVGKGSVFRVSLP 563 G GTGLGLS+ YG V + GG + + S G+ + P Sbjct: 366 DEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFP 406 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,622,794 Number of extensions: 64274 Number of successful extensions: 385 Number of sequences better than 1.0e-08: 27 Number of HSP's gapped: 385 Number of HSP's successfully gapped: 27 Length of query: 571 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 481 Effective length of database: 1,193,720 Effective search space: 574179320 Effective search space used: 574179320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)