BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0031.p (440 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 733 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 450 e-127 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 391 e-110 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 347 1e-96 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 341 7e-95 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 733 bits (1893), Expect = 0.0, Method: Compositional matrix adjust. Identities = 373/416 (89%), Positives = 397/416 (95%) Query: 22 MEVTYKKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAIYGLGAGIFFIGYF 81 +EVTY+KIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEA+YGLGAGIFFIGYF Sbjct: 21 LEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYF 80 Query: 82 LFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFMLGVFEAGFFPGV 141 +FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRF+LG+FEAGFFPGV Sbjct: 81 IFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGV 140 Query: 142 VLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGTIMSQMEGVGHLANWQWLFILEGI 201 VLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAG IMS MEGVGHLANWQWLFILEGI Sbjct: 141 VLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGI 200 Query: 202 PSLIAGVFVLLYLPEKPANAKWLSAAEQQAVTQALVAENTSASKHLSFKDACSNYRVWLC 261 PS++AG VL +LPE+PANAKWLS AE+ AVTQ L AEN++ KH SFKDAC+NYRVW+C Sbjct: 201 PSILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWIC 260 Query: 262 AAVYFCVVSGNATIAFWTPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGIHSDKTGE 321 AAVYFCVVSGNATIAFW PSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNG HSDKTGE Sbjct: 261 AAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGE 320 Query: 322 RRMHSAIATLIASVGLILTGLTLGNAVMALCALTLAAIGILAAFPVFWSIPAAFLAGTAA 381 RR+HSAIATLIA +GL+ TG+TLGNA +ALC+LTLAAIGILAAFPVFWSIP AFLAGTAA Sbjct: 321 RRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAA 380 Query: 382 AGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALEFLASFLVVVFVKK 437 AGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALE +ASFLVVVF++K Sbjct: 381 AGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVFIRK 436 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 450 bits (1157), Expect = e-127, Method: Compositional matrix adjust. Identities = 218/400 (54%), Positives = 291/400 (72%), Gaps = 1/400 (0%) Query: 23 EVTYKKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAIYGLGAGIFFIGYFL 82 + TY K+ WRL+P L +V +++DRVNVGFAKLQMLQDL+FS+ +YGLGAGIFF+GYF+ Sbjct: 18 DATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFV 77 Query: 83 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFMLGVFEAGFFPGVV 142 FEVPSN++L K+GAR +ARI I WG+ S AMAYVTTP FYI+RF+LGV EAGFFPGV+ Sbjct: 78 FEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVI 137 Query: 143 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGTIMSQMEGVGHLANWQWLFILEGIP 202 LYLTYW+PA RR ++ LFMT A++G +GGP++G IM M GV WQW+FILE IP Sbjct: 138 LYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIP 197 Query: 203 SLIAGVFVLLYLPEKPANAKWLSAAEQQAVTQALVAENTSASKHLSFKDACSNYRVWLCA 262 SLI GV V+LY+ ++ +A WLS E++ + +A ++ S +HLS +N RVWL A Sbjct: 198 SLILGVMVILYMKDRIRDADWLS-EEEKRLLEANISHEESQKEHLSLGAMFANGRVWLGA 256 Query: 263 AVYFCVVSGNATIAFWTPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGIHSDKTGER 322 +YFC V G ++FW P+IIK GV L +GL++AIP+ AM+ G +D ER Sbjct: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 Query: 323 RMHSAIATLIASVGLILTGLTLGNAVMALCALTLAAIGILAAFPVFWSIPAAFLAGTAAA 382 R H AI I +GL+L+ N +A+ +LTLA++GIL P+FWS+P AFL+GTAAA Sbjct: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAA 376 Query: 383 GGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAA 422 GIALINS+GNLAGFV+P+++G LK +T S ++G+Y +AA Sbjct: 377 AGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAA 416 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 391 bits (1005), Expect = e-110, Method: Compositional matrix adjust. Identities = 197/403 (48%), Positives = 273/403 (67%) Query: 26 YKKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAIYGLGAGIFFIGYFLFEV 85 YKK+ WR++PFL+ +V+A++DRVNVGFAKLQM QDL FSE ++GLGAG+FFIGYFLFEV Sbjct: 22 YKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEV 81 Query: 86 PSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFMLGVFEAGFFPGVVLYL 145 PSN+L+ K+GAR +ARI I WG+ S A +V T FYILRF+LG+ EAGF+PG++LYL Sbjct: 82 PSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYL 141 Query: 146 TYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGTIMSQMEGVGHLANWQWLFILEGIPSLI 205 TYWFP+ RRA+V +FM+ +AG +G P++G IM G L WQW+F++E IP+++ Sbjct: 142 TYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVL 201 Query: 206 AGVFVLLYLPEKPANAKWLSAAEQQAVTQALVAENTSASKHLSFKDACSNYRVWLCAAVY 265 GV +LYL +AKWL+ E+ ++ + + F + RVWL +Y Sbjct: 202 IGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDARVWLMCLIY 261 Query: 266 FCVVSGNATIAFWTPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGIHSDKTGERRMH 325 F V G + W P+++K GVKGNL+IGL+SAIPF +AM G +D+ ERR H Sbjct: 262 FSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRERRWH 321 Query: 326 SAIATLIASVGLILTGLTLGNAVMALCALTLAAIGILAAFPVFWSIPAAFLAGTAAAGGI 385 I L+ VG + L N +++ +L+LAA G+L P+FWS+P AFL+G AAA GI Sbjct: 322 LVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAAVGI 381 Query: 386 ALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALEFLAS 428 A INS+GNLAGFV+PY+IG LK T + ++G+Y +A + + S Sbjct: 382 AAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGS 424 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 347 bits (889), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 178/397 (44%), Positives = 257/397 (64%), Gaps = 5/397 (1%) Query: 23 EVTYKKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAIYGLGAGIFFIGYFL 82 + Y+K+ R+IPFL +V++++DR+N+GFA+LQM QDL FS+A+YGLGA IF++GY L Sbjct: 22 DAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAIFYVGYVL 81 Query: 83 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFMLGVFEAGFFPGVV 142 FEVPSN+ L + GAR+T +RI +LWGLAS+ M VT FY LRF+LGVFEAGFFPG+V Sbjct: 82 FEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAHFYTLRFLLGVFEAGFFPGIV 141 Query: 143 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGTIMSQMEGVGHLANWQWLFILEGIP 202 LYL+YWFPA+RRA V +F A+AG +GG ++G IM M GV L WQW+F +EG P Sbjct: 142 LYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGAP 201 Query: 203 SLIAGVFVLLYLPEKPANAKWLSAAEQ---QAVTQ--ALVAENTSASKHLSFKDACSNYR 257 +++ G+ + L + PA AKWLS E+ QA Q AL + S + + A S+ Sbjct: 202 AVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEALRTHHVSGHSLAALRQALSHRT 261 Query: 258 VWLCAAVYFCVVSGNATIAFWTPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGIHSD 317 +L A +YF + G+ T+ FW P +I++ G+K +++ L S IP G + +I SD Sbjct: 262 TYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSLYSVIPNAIGAVGLILIARRSD 321 Query: 318 KTGERRMHSAIATLIASVGLILTGLTLGNAVMALCALTLAAIGILAAFPVFWSIPAAFLA 377 + ER H A+ T ++ L L + ++L L++AA+ I AA P+FWS+P L Sbjct: 322 RRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLLP 381 Query: 378 GTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLS 414 A GIA I+S+G +G V+P++IG +KT TG++ Sbjct: 382 AQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMD 418 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 341 bits (874), Expect = 7e-95, Method: Compositional matrix adjust. Identities = 186/428 (43%), Positives = 270/428 (63%), Gaps = 10/428 (2%) Query: 19 ALDMEVTYKKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAIYGLGAGIFFI 78 A+ + YKK+ W+++P+L+ +V+A++DRVNVGFAKL ML DL SEA YGLGAGIFF+ Sbjct: 9 AVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFFL 68 Query: 79 GYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAY---------VTTPTSFYILRFM 129 GYF+FEVPSNL+L ++GA+ +ARI I WG+ S MA+ V+ T FY LRF+ Sbjct: 69 GYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRFL 128 Query: 130 LGVFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGTIMSQMEGVGHL 189 LG EAGFFPGV+LY+TYW+PA +RV + + + +GGPI+G +M M L Sbjct: 129 LGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDSL 188 Query: 190 ANWQWLFILEGIPSLIAGVFVLLYLPEKPANAKWLSAAEQQAVTQALVAENTSASKHLSF 249 A WQW+F++E +P+ + G+ +L L A+AKWL+ E+ + + L AEN ++ Sbjct: 189 AGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKE-KVNVPL 247 Query: 250 KDACSNYRVWLCAAVYFCVVSGNATIAFWTPSIIKEIGVKGNLQIGLISAIPFIAGTLAM 309 K + +W A VY ++ G+ I FW P+II++ GVK L IG+ISAIP+I G AM Sbjct: 248 KSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATAM 307 Query: 310 IWNGIHSDKTGERRMHSAIATLIASVGLILTGLTLGNAVMALCALTLAAIGILAAFPVFW 369 ++ H++K G+ R +S +++A + L+L L N + L + +A G L + +FW Sbjct: 308 VFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALFW 367 Query: 370 SIPAAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALEFLASF 429 P A+ G AA GIA+INS+GNL GFV PY++GAL T+ GS GL + FLA Sbjct: 368 GFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLAGV 427 Query: 430 LVVVFVKK 437 L+ + +K Sbjct: 428 LIALGGRK 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,157,265 Number of extensions: 42765 Number of successful extensions: 268 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 241 Number of HSP's successfully gapped: 20 Length of query: 440 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 352 Effective length of database: 1,203,190 Effective search space: 423522880 Effective search space used: 423522880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)