BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0027.p (351 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311166|ref|YP_003775258.1| transcription regulator protein... 452 e-128 gi|300311738|ref|YP_003775830.1| RpiR family transcription regul... 77 2e-15 gi|300312817|ref|YP_003776909.1| transcription regulator protein... 69 8e-13 >gi|300311166|ref|YP_003775258.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 452 bits (1163), Expect = e-128, Method: Compositional matrix adjust. Identities = 224/322 (69%), Positives = 260/322 (80%) Query: 30 KITSLQGGGAVARVLGKLYPNLSKAHRKTADYVLANTFRAATMTIDELSEAAGISLATAN 89 K SLQG GAVA+ +GKLY LSK+HRKTADYVL N FRAATMTIDELSEAAGIS+ATAN Sbjct: 6 KNISLQGAGAVAQAVGKLYAQLSKSHRKTADYVLGNPFRAATMTIDELSEAAGISVATAN 65 Query: 90 RFAHALGFDGYAPFRNALVADFASSLAPVEKMRHEVQKSATSVEIMSAALSNDIRNIEAT 149 RFAHALGFDGYA FR ALV+DFA++LAPVEK+R+EVQ++ATS EIM+AA +DI NIEAT Sbjct: 66 RFAHALGFDGYASFRAALVSDFATTLAPVEKLRNEVQRAATSAEIMAAAFDSDIANIEAT 125 Query: 150 LQSLVPGHCDQAVEMILKAERIFVVGFGASAFLAGIMVHALEPFCRTVLSGVHPGGPSQT 209 + L C+ AV+MIL AERIF+ GFGASA+LAG+M HALEP+ RTV S GGPSQ Sbjct: 126 RRMLTASDCEAAVDMILAAERIFITGFGASAYLAGLMAHALEPYVRTVSSVALAGGPSQA 185 Query: 210 GRQFFKLDERDVVIAMAYPRYATDTVRLARRAVEKGAKLIAFTDLPHSPLVPLADVTIYS 269 GRQFFKLD RD+VI M +PRYA DTV L +RAVEKGA+++A TD P SPLVPLADVTIY+ Sbjct: 186 GRQFFKLDNRDLVIPMVFPRYAADTVWLTQRAVEKGARILAITDSPGSPLVPLADVTIYA 245 Query: 270 QTERQLSPTSDAAALVLIQALCDAVAHRAKRSVQAASEMTEFVLPWLYQAEQHEPVRVAK 329 QT+R+LSPTSDAAAL+LIQALCDAVAHRA+RSVQAAS+M EFVLPWLY +Q + V+K Sbjct: 246 QTDRKLSPTSDAAALILIQALCDAVAHRARRSVQAASQMAEFVLPWLYLPQQQQRPVVSK 305 Query: 330 RTAAATATATANVKSAIRNRKS 351 A TA S R K+ Sbjct: 306 AAGRAKTAKTAKDVSTRRKTKA 327 >gi|300311738|ref|YP_003775830.1| RpiR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 281 Score = 77.0 bits (188), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 79/269 (29%), Positives = 131/269 (48%), Gaps = 17/269 (6%) Query: 48 YPNLSKAHRKTADYVLANTFRAATMTIDELSEAAGISLATANRFAHALGFDGY----APF 103 Y +LS+ + A YV + I +++E + + RFA GF G+ A F Sbjct: 8 YHSLSRQLKVIATYVEQHKTSLMLERISDIAEQCQVQPSAIVRFAKHFGFSGFSEMQAVF 67 Query: 104 RNALVADFASSLAPVEKMRHEVQKSATSVEIMSAALSND-IRNIEATLQSLVPGHCDQ-- 160 R A A S ++R+ +Q T V S AL+++ I++ A L L+ G ++ Sbjct: 68 REAYTAQAIPSRNYQRRIRNLIQSKPTRV--TSGALAHEFIQSSRAGLDELLAGFDEEQF 125 Query: 161 --AVEMILKAERIFVVGFGASAFLAGIMVHALEPFCRTV--LSGVHPGGPSQTGRQFFKL 216 AV+++LKAE I+V+G S +A + +AL+ + V +SG GG + + + Sbjct: 126 EAAVKLLLKAENIYVIGVRRSFPIANHLAYALQHTNKRVHLISG--AGGMFR--EEVRSI 181 Query: 217 DERDVVIAMAYPRYATDTVRLARRAVEKGAKLIAFTDLPHSPLVPLADVTIYSQTERQLS 276 D+ DV+IA+++ Y +T R A + A+ + TD SPL A V + + S Sbjct: 182 DKHDVLIAISFTPYGQETQACVRLAHHRQARTLVITDSQLSPLARHASVLLKVKESSAFS 241 Query: 277 PTSDAAALVLIQALCDAVAHRAKRSVQAA 305 S A+ L QAL A+A+R + V+ A Sbjct: 242 FRSLTNAMCLSQALFIALAYRMELDVEEA 270 >gi|300312817|ref|YP_003776909.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 293 Score = 68.6 bits (166), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 68/239 (28%), Positives = 108/239 (45%), Gaps = 9/239 (3%) Query: 59 ADYVLANTFRAATMTIDELSEAAGISLATANRFAHALGFDGYAPFRNALVADFASSLAPV 118 A ++L N A T+ LS+ A + +T R A LGF G+A R V A Sbjct: 33 AAFLLDNPGEVAVSTMRALSQKAQVQPSTFVRLAQHLGFAGWAELRALFVERLAGEPKAY 92 Query: 119 EKMRHEVQKSATSVEIMSAALSNDIRNIEATLQSLVPGHCDQ---AVEMILKAERIFVVG 175 + + + S ++++ + N++A+ P D+ A +LKA + + G Sbjct: 93 AFKAKSIVRRSNSDKLVAELFTAQRANLDAS----EPKSADRMLGAARALLKAHHVHIAG 148 Query: 176 FGASAFLAGIMVHALEPFCRTVLSGVHPGGPSQTGRQFFKLDERDVVIAMAYPRYATDTV 235 F A AF ++H L RT +S V G + + D VI +++ Y+T+ V Sbjct: 149 FRA-AFSISFLLHYLYRLFRTSVSLVR-GSEGSIELELRAIAAHDAVIVVSFAPYSTEAV 206 Query: 236 RLARRAVEKGAKLIAFTDLPHSPLVPLADVTIYSQTERQLSPTSDAAALVLIQALCDAV 294 R+A A + GAKLIA TD +P+ AD I E S AAA + ++L + V Sbjct: 207 RVAEAAKQAGAKLIALTDSAVAPIALKADHCILFSHESPSFFPSMAAATAIAESLIELV 265 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.130 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 953,759 Number of extensions: 36003 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 5 Length of query: 351 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 265 Effective length of database: 1,212,660 Effective search space: 321354900 Effective search space used: 321354900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)