BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0021.p (301 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311171|ref|YP_003775263.1| dipeptide/oligopeptide/nickel A... 464 e-132 gi|300312479|ref|YP_003776571.1| dipeptide/oligopeptide/nickel A... 169 2e-43 gi|300310894|ref|YP_003774986.1| dipeptide/oligopeptide/nickel A... 167 1e-42 gi|300310465|ref|YP_003774557.1| dipeptide/oligopeptide/nickel A... 127 8e-31 gi|300312717|ref|YP_003776809.1| ABC transporter permease [Herba... 124 9e-30 >gi|300311171|ref|YP_003775263.1| dipeptide/oligopeptide/nickel ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 310 Score = 464 bits (1193), Expect = e-132, Method: Compositional matrix adjust. Identities = 219/286 (76%), Positives = 256/286 (89%) Query: 16 ASKPIRTPWSEFWRKFRKQPLALAAGAFVLFLVVIAIVSPYISPYDAENYFDYDALNAGP 75 + + IRTPW EFWRKF+KQ LA+ AG F+LFLV +AI++P+I P+D EN+FDYDALN+ P Sbjct: 25 SGQKIRTPWREFWRKFKKQHLAVYAGVFILFLVAVAILAPWIVPFDPENFFDYDALNSPP 84 Query: 76 SLAHWFGVDSLGHDIFSRILVGTQLSLAVGFSSVAVGAIVGTFLGLVAGYYEGWADRVIM 135 + HWFGVDSLG DIFSRI++GT++SLA GF SVA+GA++GT GL+AGYYEGW DR+ M Sbjct: 85 TWVHWFGVDSLGRDIFSRIVMGTRISLAAGFFSVAIGAVIGTVFGLLAGYYEGWWDRITM 144 Query: 136 RICDVLFAFPGILLAIAIVAILGNGMTNVIIAVAIFSIPAFARLVRGNTLSLKQLTYIEA 195 RICDVLFAFPGILLAI IVAILG GM NVII+V++FSIPAFARLVRGNTL LK LTYIEA Sbjct: 145 RICDVLFAFPGILLAIGIVAILGGGMINVIISVSVFSIPAFARLVRGNTLQLKNLTYIEA 204 Query: 196 VRSIGANDMTIILRHIFPGTISSVVVYFTMRIGTSIITAASLSFLGLGAQPPTPEWGAML 255 RSIGA+DMTI+ +HIFPGTIS++VVYFTMRIGTSIITAA LSFLGLGAQ PTPEWG ML Sbjct: 205 TRSIGASDMTIMWKHIFPGTISAIVVYFTMRIGTSIITAAGLSFLGLGAQSPTPEWGLML 264 Query: 256 NEARADMMTAPHIAIFPSLAIFLTVLAFNLLGDGLRDALDPKLDQK 301 NEARADM+T+PH+A+ PS+AIFLTVLAFNLLGDGLRDALDPK+DQ+ Sbjct: 265 NEARADMVTSPHVALVPSIAIFLTVLAFNLLGDGLRDALDPKIDQR 310 >gi|300312479|ref|YP_003776571.1| dipeptide/oligopeptide/nickel ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 305 Score = 169 bits (429), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 103/281 (36%), Positives = 161/281 (57%), Gaps = 15/281 (5%) Query: 28 WRKFRKQPLA---LAAGAFVLFLVVIA-IVSPYISPYDAENYFDYDAL--------NAGP 75 WR+ ++ L L GA VL ++V+A I +P ++P+D FD D A Sbjct: 24 WRRKLRRLLGHRGLMIGAAVLAVIVLAAIFAPLLAPHDP---FDQDVTARLIPPVWQAQG 80 Query: 76 SLAHWFGVDSLGHDIFSRILVGTQLSLAVGFSSVAVGAIVGTFLGLVAGYYEGWADRVIM 135 S H G D LG D FSR++ G ++SL +G ++ + ++GT LG++AGY+ G D VI Sbjct: 81 SWEHILGTDKLGRDYFSRLIYGARVSLTIGLAAALISGLIGTTLGVLAGYFGGRTDAVIS 140 Query: 136 RICDVLFAFPGILLAIAIVAILGNGMTNVIIAVAIFSIPAFARLVRGNTLSLKQLTYIEA 195 + A P +L+A+A+ +++G + VI+ + + FA + R T L+ +I A Sbjct: 141 YLITTRLAMPVVLVALAMASLVGGSLKVVIVLLGLLLWDRFAVVTRSATQQLRDAEFIAA 200 Query: 196 VRSIGANDMTIILRHIFPGTISSVVVYFTMRIGTSIITAASLSFLGLGAQPPTPEWGAML 255 ++IGA+ I+LR I P + +++V T+ + +I+ A+LSFLGLG QPPTP WG M+ Sbjct: 201 AKAIGASTPYILLREILPNIMGALIVVVTLEMAHAILLEATLSFLGLGVQPPTPSWGLMV 260 Query: 256 NEARADMMTAPHIAIFPSLAIFLTVLAFNLLGDGLRDALDP 296 E + M P + + P +A+ L VLA NL+GDGLRD P Sbjct: 261 AEGKTYMFFKPWVIVIPGVALALLVLAINLVGDGLRDVNAP 301 >gi|300310894|ref|YP_003774986.1| dipeptide/oligopeptide/nickel ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 320 Score = 167 bits (422), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 91/290 (31%), Positives = 157/290 (54%), Gaps = 10/290 (3%) Query: 21 RTPWSEFWRKFRKQPLALAAGAFVLFLVVIAIVSPYISPYDAENYFDYD---------AL 71 +PW R F A+ VL L+V AI +P+I+P + + D L Sbjct: 31 ESPWRRVMRHFVASRSAMLGLVIVLLLIVGAIAAPWITPQNPYDLMQLDVLDARLHPGTL 90 Query: 72 NAGPSLAHWFGVDSLGHDIFSRILVGTQLSLAVGFSSVAVGAIVGTFLGLVAGYYEGWAD 131 N + +W G D G D+ S IL G ++S+ +G S + ++GT +GL+A Y G D Sbjct: 91 NGAGTYTYWLGTDGQGRDLVSAILYGLRISVGIGVGSALIAGVIGTLIGLLAAYAGGKLD 150 Query: 132 RVIMRICDVLFAFPGILLAIAIVAILGNGMTNVIIAVAIFSIPAFARLVRGNTLSLKQLT 191 ++MR D++ +FP IL+A+ I+A +G G+ NV+ + + +AR RG L ++ Sbjct: 151 TLLMRFADLVLSFPSILVAMLILAYVGKGIFNVMATLVVLEWAYYARTARGQALVERRKE 210 Query: 192 YIEAVRSIGANDMTIILRHIFPGTISSVVVYFTMRIGTSIITAASLSFLGLGAQPPTPEW 251 Y+EA R + + I++RHI P + ++V T+++ +I A+LSFLGLG P Sbjct: 211 YVEAARGLNLSSWRIMVRHILPNCLPPLIVIGTLQVARAITLEATLSFLGLGVPITEPSL 270 Query: 252 GAMLNEARADMMTAPH-IAIFPSLAIFLTVLAFNLLGDGLRDALDPKLDQ 300 G +++ M++ + I+ +P + + + ++A NL+GD LRD L+P+ + Sbjct: 271 GLLISNGYQYMLSGEYWISFYPGITLLVVIVAINLVGDRLRDILNPRWQK 320 >gi|300310465|ref|YP_003774557.1| dipeptide/oligopeptide/nickel ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 127 bits (320), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 87/269 (32%), Positives = 137/269 (50%), Gaps = 1/269 (0%) Query: 29 RKFRKQPLALAAGAFVLFLVVIAIVSPYISPYDAENYFDYDALNAGPSLAHWFGVDSLGH 88 R R +A A V F +++AI+ P ISPYDA D D + G S G D LG Sbjct: 25 RVRRHSTMAWIGMAIVGFWILMAILGPSISPYDATAIVDTDVFS-GMSHKFLLGSDYLGR 83 Query: 89 DIFSRILVGTQLSLAVGFSSVAVGAIVGTFLGLVAGYYEGWADRVIMRICDVLFAFPGIL 148 D+ SRIL GT+ ++ + ++ + + GT + L A + GW D VI R D L + P + Sbjct: 84 DMLSRILFGTRATIGLALAATVLASATGTAIALFAAFSGGWRDAVISRALDALISIPSKM 143 Query: 149 LAIAIVAILGNGMTNVIIAVAIFSIPAFARLVRGNTLSLKQLTYIEAVRSIGANDMTIIL 208 A+ +VA G+ M +++ AI +P R+ R ++++ + Y++A R+ G I+ Sbjct: 144 FALMMVATFGSSMLLLVLTAAITYMPGAYRIARSLAVNVQAMEYVQAARARGEGVWYIMT 203 Query: 209 RHIFPGTISSVVVYFTMRIGTSIITAASLSFLGLGAQPPTPEWGAMLNEARADMMTAPHI 268 + P I V+ F +R ++ + LSFL LG QPP +WG+++ E + + Sbjct: 204 VEMLPNMIRPVLADFGLRFVYVVLLLSGLSFLSLGVQPPDADWGSLVRENISGLGEGALA 263 Query: 269 AIFPSLAIFLTVLAFNLLGDGLRDALDPK 297 I P+LAI + NLL D LR K Sbjct: 264 VIMPALAIASLTIGVNLLIDNLRSKRAAK 292 >gi|300312717|ref|YP_003776809.1| ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 348 Score = 124 bits (311), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 74/236 (31%), Positives = 129/236 (54%), Gaps = 1/236 (0%) Query: 60 YDAENYFDYDALNAGPSLAHWFGVDSLGHDIFSRILVGTQLSLAVGFSSVAVGAIVGTFL 119 Y+ NYF A PS +W G D G D+F+R+L G ++S+ G + +G ++G Sbjct: 109 YNTLNYFAKQPNPAAPSSENWLGTDDRGRDVFARLLYGFRVSVLFGMALTFIGVVLGVAA 168 Query: 120 GLVAGYYEGWADRVIMRICDVLFAFPGILLAIAIVAILGNGMTNVIIAVAIFSIPAFARL 179 G V GY+ G D R+ ++ + P + L I +I + +++ +++F + + Sbjct: 169 GAVQGYFAGRTDLFTQRLLEIWGSMPELYLLIIFSSIFQPSLGLLLVLLSLFGWMSLSDY 228 Query: 180 VRGNTLSLKQLTYIEAVRSIGANDMTIILRHIFPGTISSVVVYFTMRIGTSIITAASLSF 239 VR + L + L YI+A R++G D II RH+ P ++++VV + R+ +I+ SL F Sbjct: 229 VRADFLRNRNLEYIQAARAMGLPDRLIIWRHVLPNSMTTVVTFLPFRMSAAILALTSLDF 288 Query: 240 LGLGAQPPTPEWGAMLNEARADMMTAPHIAIFPSLAIFLTVLAFNLLGDGLRDALD 295 LGLG P TP G +L + + + + A IA+ + + +T+L +G+ LR+ALD Sbjct: 289 LGLGVPPSTPSLGELLAQGKNN-LDAWWIALSTFVVLTVTLLLLTNIGNALRNALD 343 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 842,571 Number of extensions: 32180 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 6 Length of query: 301 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 216 Effective length of database: 1,217,395 Effective search space: 262957320 Effective search space used: 262957320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)