BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0016.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase o... 681 0.0 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 125 8e-30 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 121 9e-29 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 110 1e-25 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 96 4e-21 >gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 708 Score = 681 bits (1758), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/386 (87%), Positives = 362/386 (93%) Query: 1 VLDFSPEAPDFDRLVDSLDKALEDYRHDYSAYYQRCRRANSPAMRDANPIIYLIPGVGML 60 VLDFSP+ PD L SLD ALE YR DY+AYYQRC R NSPAMRD NP+IYLIPGVGML Sbjct: 323 VLDFSPQQPDLAALEASLDAALEAYRQDYAAYYQRCARPNSPAMRDPNPVIYLIPGVGML 382 Query: 61 SFAKDKATARIAGEFYLNAINVMRGANSVDTYVGLPEQEAFDIEYWLLEEAKLQRMPKPK 120 SFAKDKATARIAGEFY+NA+NVMRGAN VD+YVGLPEQEAFDIEYWLLEEAKLQRMPKPK Sbjct: 383 SFAKDKATARIAGEFYINAVNVMRGANGVDSYVGLPEQEAFDIEYWLLEEAKLQRMPKPK 442 Query: 121 SLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSSIDSAYQSLLKQAGPDAIGVV 180 SLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQS++DSA QSLLK GPDAIGVV Sbjct: 443 SLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSALDSAQQSLLKHGGPDAIGVV 502 Query: 181 RADITNEQDVENVISNVALRFGGIDLLVSNAGIASASPLEDTSLEVWQRNMSVLATGYFL 240 RADIT+E DV ++++VALRFGGIDLLVSNAGIASASPL+DTSLEVWQRN+SVLATGYFL Sbjct: 503 RADITSEADVTTILNSVALRFGGIDLLVSNAGIASASPLQDTSLEVWQRNLSVLATGYFL 562 Query: 241 VSRSAFRSMTAQAMGGSIVFVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIR 300 VSR+AF SM Q +GGSIV+VASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIR Sbjct: 563 VSRAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIR 622 Query: 301 VNVVNPDAVIRGSRIWDGKWKEERAASNKIDSDNIEEFYRQRSLLKRSVLPEDIAEAVYF 360 VNVVNPDAVIRGSRIWDGKWKEERAASNKI++D++EEFYRQRS+LKRSVLPEDIAEAVYF Sbjct: 623 VNVVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEFYRQRSMLKRSVLPEDIAEAVYF 682 Query: 361 FASEKSAKSTGNILNVDAGNAAAFTR 386 FASEKSAKSTGNILNVDAGNA AFTR Sbjct: 683 FASEKSAKSTGNILNVDAGNAGAFTR 708 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 125 bits (313), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 86/258 (33%), Positives = 139/258 (53%), Gaps = 22/258 (8%) Query: 125 RIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSSIDSAYQSLL---KQAGPDAIGVVR 181 R+ALVTGG GIG ++++QL A+G V++ DI+ + +L+ +A P ++ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAP-----LQ 63 Query: 182 ADITNEQDVENVISNVALRFGGIDLLVSNAGIASASPLEDTSLEVWQRNMSVLATGYFLV 241 D+ + + + +++ +G D+LV+NAG+A D LE W + M+V TG L Sbjct: 64 MDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLA 123 Query: 242 SRSAFRSMTAQAMGGSIVFVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIRV 301 + A R M Q G IV ++S +G+ A AG +AY T+KAA I L R +A+E A +GI V Sbjct: 124 GQHAARLMVKQQWG-RIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITV 182 Query: 302 NVVNPDAVIRGSRIWDGKWKEERAASNKIDSDNIEEFYRQRSLLKRSVLPEDIAEAVYFF 361 N V P V + + + S+ E Y + ++R ++IA AV + Sbjct: 183 NSVAPGPV-------------DTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYL 229 Query: 362 ASEKSAKSTGNILNVDAG 379 A+E+SA TG+++ VD G Sbjct: 230 AAEESAYITGHVIPVDGG 247 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 121 bits (304), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 74/258 (28%), Positives = 140/258 (54%), Gaps = 4/258 (1%) Query: 122 LAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSSIDSAYQSLLKQAGPDAIGVVR 181 L ++AL+TG GIG+ +A + +GA V++ D+ + + + ++K+ G V Sbjct: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAM--AVA 60 Query: 182 ADITNEQDVENVISNVALRFGGIDLLVSNAGIASASPLEDTSLEVWQRNMSVLATGYFLV 241 ++T+E V+ +++ +GG+D+++SNAGI SP+ + SLE W++ +++ G FL Sbjct: 61 MNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLT 120 Query: 242 SRSAFRSMTAQAMGGSIVFVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIRV 301 +R+A ++M Q GGSI+++ S + +AS + Y TAK + LA+ +A EGA IR Sbjct: 121 TRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRT 180 Query: 302 NVVNPDAVIRGSRIWDGKWKEERAASNKIDSDNIEEFYRQRSLLKRSVLPEDIAEAVYFF 361 NV+ P V + + + + E+ + D I++ + ++ +D+A+ F Sbjct: 181 NVICPGFV--RTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFL 238 Query: 362 ASEKSAKSTGNILNVDAG 379 A+ + TG + V G Sbjct: 239 AAFPTNALTGQSVVVSHG 256 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 110 bits (276), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 81/272 (29%), Positives = 128/272 (47%), Gaps = 24/272 (8%) Query: 108 LEEAKLQRMPKPKSLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSSIDSAYQS 167 L ++LQR G++ +VTG GIG+A AR+ EGA V+L D D +++ + S Sbjct: 4 LNPSRLQR------FEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDS 57 Query: 168 LLKQAGPDAIGVVRADITNEQDVENVISNVALRFGGIDLLVSNAGIASASPLEDTSLEVW 227 L P+ D+++ + V ++ RFG +D+LVS+AG+ + + + S E W Sbjct: 58 L----PPERTAARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDW 113 Query: 228 QRNMSVLATGYFLVSRSAFRSMTAQAMGGSIVFVASKNGLVASAGASAYCTAKAAEIHLA 287 R + G F +R A + + G IV VAS +GL A SAY +K A +L Sbjct: 114 HRVQATNVNGVFYGAREALPHL--EKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLT 171 Query: 288 RCIALEGAPHGIRVNVVNPDAVIRGSRIWDGKWKEERAASNKIDSDNIEEFYRQRSLLKR 347 R +AL+ G+R+N V P ++ D + + + +R L R Sbjct: 172 RAMALDFGRKGVRINAVCP------------SLTHTAMTADMADDPPLLDKFAERIALGR 219 Query: 348 SVLPEDIAEAVYFFASEKSAKSTGNILNVDAG 379 P +IA + F AS ++ G L VD G Sbjct: 220 GADPLEIAAVITFLASPDASFVNGVNLPVDGG 251 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 96.3 bits (238), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 82/269 (30%), Positives = 125/269 (46%), Gaps = 31/269 (11%) Query: 121 SLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSSIDSAYQSLLKQAGPDAIGVV 180 +L G++AL+TG GIG+A A GA ++LT Q +++ + Q G + VV Sbjct: 3 TLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRAS--VV 60 Query: 181 RADITNEQDVENVISNVALRFGGIDLLVSNAG-IASASPLEDTSLEVWQRNMSVLATGYF 239 D+ E ++ FGG+D+ ++NAG + + PL + S + WQ + T F Sbjct: 61 AGDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAF 120 Query: 240 LVSRSAFRSMTAQAMGGSIVFVASKNGLVAS-AGASAYCTAKAAEIHLARCIALEGAPHG 298 L +R +M A+ G+IVF +S G A G +AY AKA + L + IA + A G Sbjct: 121 LGARCQIPAMLARGA-GAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQG 179 Query: 299 IRVNVVNPDAVIRGSRIWDGKWKEERAASNKIDSD-----NIEEFYRQRSLLKRSVLPED 353 IR N + P V D+D +++ +KR PE+ Sbjct: 180 IRANALLPGGV---------------------DTDMGGDPQQKQWAAGLHAMKRIAQPEE 218 Query: 354 IAEAVYFFASEKSAKSTGNILNVDAGNAA 382 IA A F AS ++ TG L D GN+A Sbjct: 219 IAAAALFLASPMASFVTGTALYADGGNSA 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,087,325 Number of extensions: 41263 Number of successful extensions: 258 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 42 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)