BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0025.0231.p
         (464 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbas...    74   2e-14

>gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 471

 Score = 74.3 bits (181), Expect = 2e-14,   Method: Compositional matrix adjust.
 Identities = 78/347 (22%), Positives = 134/347 (38%), Gaps = 80/347 (23%)

Query: 5   HLNAWLKPYGLWFPVDVSTAAQCTIGGMSGNNSCGSRSIEYGNMVHNVIAIDAVLADGTE 64
            LN  +K  GL+FP+D    A  ++GGM+   + G+ ++ YG M  N + +  V A    
Sbjct: 128 QLNQEIKDTGLFFPID--PGADASLGGMASTRASGTNAVRYGTMKENTLTLTVVTA---- 181

Query: 65  GRFGRLPDMTEMPEMPGRGRIGEIVRGLQAIAQRERGEIVERVPKVLRRVAGYNIDIFDC 124
                                              +GEI++   +  +  AGY+      
Sbjct: 182 -----------------------------------QGEIIKTGTRAKKSSAGYD------ 200

Query: 125 QNPRAYTDDGVANLAHILIGSEGTLAYSRQITLALMPLPAHKVLGVVNFPTFYQSMDMTQ 184
                        L  + +GSEGTL    ++T+ L P P      + +FPT   +++   
Sbjct: 201 -------------LTRVYVGSEGTLGIITEVTVRLYPQPEAISAAICSFPTVADAVNTVI 247

Query: 185 HIVKLG-PTA-VELVDRTMIDLAISNPAFRPVIEKALVGHPEAILLVEFAGENLDQQLDK 242
             +++G P A VEL+D   +       A     + +L  +P  +LL EF G       + 
Sbjct: 248 QTIQMGVPLARVELLDENGVR------AINAHDKLSLPVNP--LLLFEFHGSE-----NG 294

Query: 243 LKLLSELMGDLGLPGSVVDMPVA---NEQKALWEVRKAGLNIMMSMKGDGKPVSFIEDCA 299
           +K  +EL+ D+      +    A    ++  LW  R      ++ ++   + +S   DC 
Sbjct: 295 VKEQAELVQDIAKEFHALGFEWATRPEDRTRLWTARHNAYFALLQLRPGARAIS--TDCC 352

Query: 300 VPLEHLAEYTRQLTEVFHKYGTEGTWYAHASVGTLHVRPILDMRRPA 346
           VP+  LAE          + G       H   G  HV+ ++D   PA
Sbjct: 353 VPISRLAECVLATKADCEEQGLTHAIIGHVGDGNFHVQMMVDPNDPA 399


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.135    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,465,399
Number of extensions: 59182
Number of successful extensions: 172
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 172
Number of HSP's successfully gapped: 1
Length of query: 464
Length of database: 1,619,870
Length adjustment: 89
Effective length of query: 375
Effective length of database: 1,198,455
Effective search space: 449420625
Effective search space used: 449420625
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 132 (55.5 bits)