BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0230.p (485 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 110 2e-25 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 107 2e-24 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 103 4e-23 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 97 3e-21 gi|300309866|ref|YP_003773958.1| major facilitator superfamily p... 90 3e-19 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 79/308 (25%), Positives = 136/308 (44%), Gaps = 12/308 (3%) Query: 6 LLWALATVLTGFAGGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHAF 65 +W++A + GF L L+AR+ LGLGE FPA +S W A +RG GI A Sbjct: 90 FIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGAKVVSEWFAMRERGGPTGIFVAS 149 Query: 66 ARIGNAVAPAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHPRITAEELEV 125 + IG A+AP + +M GWR F I G + + W + ++T E+ Sbjct: 150 STIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLFYRNR--NEVQLTPSEVLY 207 Query: 126 LPPGKKTSPKI-------PWGPLFKRMMPVTIVYFCYG--WTLWLFLSWIPQYFLHSYDL 176 L P W LF +++ G + +WL+L+W+P Y H L Sbjct: 208 LADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHL 267 Query: 177 DIKKSALFASSVFFAGVVGDTLGGIVSDKILRNTGNLKRARSYMVSVCMLLTLLSLLPLM 236 I ++ + G +G G ++D +++ R+R + + ++ +P Sbjct: 268 SIARTGWVVVIPYIFGTLGMLSSGYIADGLMKRGMPAIRSRKWPICAGLIGAGAFTIPAA 327 Query: 237 FTHNLYVSIISLSAGFFFAEMTIGPMWA-IPMDIAPEYSGTASGMMNTGSALAAIISPVL 295 +T + ++I+ +S F M G WA + + I + M N G L ++PV+ Sbjct: 328 YTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLVASLGSMQNFGGYLGGSLAPVI 387 Query: 296 SGYLIDKT 303 +G ++D+T Sbjct: 388 TGVVVDQT 395 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 107 bits (268), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 82/323 (25%), Positives = 146/323 (45%), Gaps = 25/323 (7%) Query: 18 AGGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHAFARIGNAVAPAAV 77 A + SL L R ++GL E +FPA ++ W +RG A I ++ + + Sbjct: 105 AAAVASLFLLRFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLM 164 Query: 78 VWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHPRITAEEL-------------- 123 WI+ + W F + G + ++W + P HPRI EL Sbjct: 165 AWIVHTYSWHHVFTVMGVVGIALALIWGRII-YSPKDHPRINQAELAHIESGGGLVDMDN 223 Query: 124 -EVLPPGKKTSPKIPWGPLFKRMMPVTIVYFCYGWTLWLFLSWIPQYFLHSYDLDIKKSA 182 +V P K I + ++ V I +C + FL+W P Y + + I K+ Sbjct: 224 RKVAAPSTKGRGYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAG 283 Query: 183 LFASSVFFAGVVGDTLGGIVSDKILRNTGNLKRARSYMVSVCMLLTLLSLL-PLMFTHNL 241 AS G +G +GG++SD++++ ++ AR + MLL++ +L + + Sbjct: 284 FVASIPAVCGFIGGVVGGLISDRLIKRGLSVTWARKIPIVGGMLLSMTMILCNYVEAQWM 343 Query: 242 YVSIISLSAGFFFAEMTIGPMWAIPMDIAP-EYSGTASGMMNTGSALAAIISPVLSGYLI 300 V I++L+ FF + WA+ D AP + G + G+ N +A I +P++ GY++ Sbjct: 344 VVGIMALA---FFGKGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIV 400 Query: 301 DKTGNWELPFLGSMVLMGFGVLL 323 +TG+ F G++V +G L+ Sbjct: 401 KETGS----FSGALVFVGINALV 419 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 103 bits (256), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 88/310 (28%), Positives = 140/310 (45%), Gaps = 14/310 (4%) Query: 5 GLLWALATVLTGFAGGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHA 64 L W+L FA G SL R LLG+ E A P A + W +R A G + Sbjct: 82 ALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKASTEWFPAKERSVAIGWFNI 141 Query: 65 FARIGNAVAPAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHPRITAEELE 124 + IG AP VVW + GW+ SF + GA ++W ++W +F + P ++ EE Sbjct: 142 GSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLW-MLFYKSPRDQKLLSPEERA 200 Query: 125 VLPPGKKTSP----KIPWGPLF--KRMMPVTIVYFCYGWTLWLFLSWIPQYFLHSYDLDI 178 + G++ SP + W + + + I F F +WIP Y ++I Sbjct: 201 YILEGQEKSPEKVQRESWTKIVRSRNFWSIAIPRFLSEPAWQTFNAWIPLYMATERHMNI 260 Query: 179 KKSALFASSVFFAGVVGDTLGGIVSDKILRNTGNLKRARSYMVSVCMLLTLLSLL---PL 235 K+ A+FA F A +G LGG +S + +LK + + ML+ LS++ + Sbjct: 261 KEIAMFAWLPFLAADIGCVLGGYLSPLFHK---HLKVSLFTSRKLVMLVGSLSMIGPACV 317 Query: 236 MFTHNLYVSIISLSAGFFFAEMTIGPMWAIPMDI-APEYSGTASGMMNTGSALAAIISPV 294 F + YV+I LS G F + G +++I D+ TA+G+ L A + + Sbjct: 318 GFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGYLGATLFTL 377 Query: 295 LSGYLIDKTG 304 L G L+ + G Sbjct: 378 LFGILVTQIG 387 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 97.1 bits (240), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 96/343 (27%), Positives = 153/343 (44%), Gaps = 13/343 (3%) Query: 5 GLLWALATVLTGFAGGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHA 64 G+LW++A VL A G +SL R L+GL E A P+ A++ W ++ A G ++ Sbjct: 82 GVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNS 141 Query: 65 FARIGNAVAPAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHPRITAEELE 124 +G +AP V+++ +GW+ +F + G VW +W F F P H ++ E E Sbjct: 142 GTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAALW-FAFYRSPRDHAALSDSERE 200 Query: 125 VLPPGK-----KTSPKIPWGPLF--KRMMPVTIVYFCYGWTLWLFLSWIPQYFLHSYDLD 177 + G+ K P + +R + + F F WIP YF++ LD Sbjct: 201 TIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLD 260 Query: 178 IKKSALFASSVFFAGVVGDTLGGIVSDKILRNTGNLKRARSYMVSVCM-LLTLLSLLPLM 236 +K A+FA F A +G GG +S L N + S + V + + ++ + Sbjct: 261 LKSIAMFAWLPFLAADLGGLAGGYLS-PFLINRFRIPLVWSRVCGVVLGAVMMIGPACVG 319 Query: 237 FTHNLYVSIISLSAGFFFAEMTIGPMWAIPMDI-APEYSGTASGMMNTGSALAAIISPVL 295 + YV+I G F +M G + + D+ PE GTASG + + + +L Sbjct: 320 LVASPYVAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLL 379 Query: 296 SGYLIDKTGNWEL-PFLGSMVLMGFGVLLAFRMQPDSKFENTA 337 G L D G L LG+ L+G VLL ++ S E A Sbjct: 380 VGALADTVGYGPLFGMLGAFDLIG-AVLLIILIRGQSTEERAA 421 >gi|300309866|ref|YP_003773958.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 484 Score = 90.1 bits (222), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 87/346 (25%), Positives = 153/346 (44%), Gaps = 51/346 (14%) Query: 6 LLWALATVLTGFAGGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHAF 65 LL +L T L G ++ L L R+ LGL EG T ++RW +++G A G+ A Sbjct: 121 LLTSLFTGLMGTTSSVLLLQLYRVGLGLSEGPLGIGCTNVINRWFPPEEKGTATGLWIAA 180 Query: 66 ARIGNAVAPAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHP-------RI 118 +++G + P + ++ GWRE FY+F + I+W F+ T P+++ R Sbjct: 181 SKLGPLIVPTVCIIVIQLWGWREIFYVFAVPGIFLAILWFFLVTNSPSENRFCNEAERRY 240 Query: 119 TAEELEVLPP----------GKKTSPKIPWGPLFKR------------------MMPVTI 150 +++ P G + +P +PW R ++ V I Sbjct: 241 ITQDVAAGPATATASSRAHVGAQVTP-MPWLDGINRTRRVPRLETLRQVFTSWNVIGVAI 299 Query: 151 VYFCYGWTLWLFLSWIPQYFLHSYDLDIKKSALFASSVFFAGVVGDTLGGIVSDKILRNT 210 Y C +F+SWIP Y + K AS+ F V G+ LGG++SD++L Sbjct: 300 GYGCMIGISNIFMSWIPTYLVTVKGFASVKMGFLASAPFIGAVAGNMLGGVISDRLLGG- 358 Query: 211 GNLKRARSYMVSVCMLLTLLSLLPLMFTHNLYVSIISLSAGFFFAEMTIGPMW----AIP 266 R + M+ + L++LL + S++ L A + + +G + A P Sbjct: 359 ----RRKPMMLLGALGTALMTLLLVEAPD----SVLYLGAMLMLSGLMLGIGFAGYSAYP 410 Query: 267 MDIAPEYSGTAS-GMMNTGSALAAIISPVLSGYLIDKTGNWELPFL 311 M +A + + A+ G++N+ + +P+ G L+D+ +W FL Sbjct: 411 MGLATKATYPAAFGIVNSLGQIGGACAPLAVGMLLDRY-DWSSVFL 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,411,912 Number of extensions: 55238 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 270 Number of HSP's successfully gapped: 11 Length of query: 485 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 396 Effective length of database: 1,198,455 Effective search space: 474588180 Effective search space used: 474588180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)