BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0225.p (116 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311291|ref|YP_003775383.1| endoribonuclease L-PSP protein ... 214 1e-57 gi|300313660|ref|YP_003777752.1| endoribonuclease L-PSP protein ... 77 3e-16 gi|300311080|ref|YP_003775172.1| translation initiation inhibito... 55 1e-09 >gi|300311291|ref|YP_003775383.1| endoribonuclease L-PSP protein [Herbaspirillum seropedicae SmR1] Length = 116 Score = 214 bits (546), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 103/116 (88%), Positives = 107/116 (92%) Query: 1 MTVQRLHVGKRLSEAAIHNGTVYLAGQIAEDTTQNIEGQTREVLGHIDRLLAEAGSSKEH 60 M+VQRLHVG RLSE AIHNGTVYLAGQIAEDTTQNIEGQTREVLGHIDRLLAEAGS K Sbjct: 1 MSVQRLHVGNRLSEVAIHNGTVYLAGQIAEDTTQNIEGQTREVLGHIDRLLAEAGSDKSK 60 Query: 61 ILSCQIYIADVKDFEGMNAVWDAWVASGNTPPRATVEAKLARPELLVEIIIVAAQK 116 ILSCQI+++DVKDF GMNAVWD WVA GN PPRATVEAKLARPELLVEIIIVAAQK Sbjct: 61 ILSCQIFLSDVKDFAGMNAVWDEWVAPGNAPPRATVEAKLARPELLVEIIIVAAQK 116 >gi|300313660|ref|YP_003777752.1| endoribonuclease L-PSP protein [Herbaspirillum seropedicae SmR1] Length = 116 Score = 77.4 bits (189), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 36/103 (34%), Positives = 58/103 (56%) Query: 3 VQRLHVGKRLSEAAIHNGTVYLAGQIAEDTTQNIEGQTREVLGHIDRLLAEAGSSKEHIL 62 ++R + R+S+ I NG +++GQ+ + I Q EVL ID L G +E I+ Sbjct: 4 IERFNTNARMSQLVIANGFAFVSGQVPDHAGAPIAQQAAEVLAKIDAQLQAQGIGRERIV 63 Query: 63 SCQIYIADVKDFEGMNAVWDAWVASGNTPPRATVEAKLARPEL 105 S ++++ + F NAVW+AW+ +G+ P RA V+A L P L Sbjct: 64 SANVWLSSPEHFAAFNAVWEAWLPAGHAPTRACVQALLMLPGL 106 >gi|300311080|ref|YP_003775172.1| translation initiation inhibitor protein [Herbaspirillum seropedicae SmR1] Length = 127 Score = 55.5 bits (132), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 30/109 (27%), Positives = 61/109 (55%), Gaps = 6/109 (5%) Query: 12 LSEAAIHNGTVYLAGQIAEDTTQNIEG----QTREVLGHIDRLLAEAGSSKEHILSCQIY 67 + A +G +Y++GQ+A + + I+G Q+ + + ++ +L EAG EH++ C ++ Sbjct: 21 FARAVAADGWLYVSGQVAMENGEVIDGGIVAQSHKAIQNVLAILKEAGYGPEHVVRCGVW 80 Query: 68 IADVKDFEGMNAVWDAWVASGNTPPRATVEAKLARPELLVEIIIVAAQK 116 + D +DF+ N V+ + + N P RA V + + + VE+ VA ++ Sbjct: 81 LDDARDFQSFNKVFKEYFGA-NPPARACVVSSMV-VDCKVEVDCVAFKR 127 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 363,200 Number of extensions: 13505 Number of successful extensions: 43 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 42 Number of HSP's successfully gapped: 3 Length of query: 116 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 43 Effective length of database: 1,274,215 Effective search space: 54791245 Effective search space used: 54791245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)