BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0224.p (288 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [H... 512 e-146 gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil... 97 1e-21 gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum serop... 62 7e-11 >gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 512 bits (1319), Expect = e-146, Method: Compositional matrix adjust. Identities = 244/288 (84%), Positives = 261/288 (90%) Query: 1 MTQAVLKTVQCISPAGLHSMAYKEWGDPHNPNVLVCVHGVTRVSDDFDVMARDLCDTYRV 60 MT+AV+KTVQCISPAGLH+MAYKEWGD NPNVLVCVHGVTRVSDDFD MAR+LCDTYRV Sbjct: 1 MTEAVVKTVQCISPAGLHTMAYKEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRV 60 Query: 61 VCPDVVGRGRSGRLGNPQHYAIPQYVSDMVTLLARLDADSVDWFGTSMGGLIGMGLASLP 120 +CPDVVGRGRSGRL NPQ Y +PQYVSDMVTLLARLDA VDWFGTSMGGLIGM LASLP Sbjct: 61 ICPDVVGRGRSGRLANPQFYTVPQYVSDMVTLLARLDAQIVDWFGTSMGGLIGMALASLP 120 Query: 121 DNPVRRLVLNDIGPSINGSALARIGEYIGQDVRFATFEEAALYIRTISASFGPHTDEEWH 180 NP+RRLVLNDIGPSING+ALARIG+YIGQDVRF +FEEAA YIRTISASFG H+DEEWH Sbjct: 121 GNPIRRLVLNDIGPSINGAALARIGDYIGQDVRFDSFEEAAQYIRTISASFGQHSDEEWH 180 Query: 181 KLASDVLRQDEEGKWKRHYDLRLAEPFKSMTPEAASIGEAMLWAAYDAIRCPTLLVRGAQ 240 KLA DVLRQ+E+G+W RHYDL LA PFK TPEAAS E MLWAAYDAIRCPTLLVRGAQ Sbjct: 181 KLAKDVLRQNEQGQWVRHYDLALALPFKQTTPEAASAAEQMLWAAYDAIRCPTLLVRGAQ 240 Query: 241 SDLLLPEVAQAMTQRGPKAKLVELEGVGHAPTFMHAQQIQVAKDFLLG 288 SDLLLPEVAQ MT+RGPKAKLVEL VGHAPTFMHA QI+VA+ FLLG Sbjct: 241 SDLLLPEVAQEMTRRGPKAKLVELPDVGHAPTFMHAPQIEVARQFLLG 288 >gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 97.4 bits (241), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 80/270 (29%), Positives = 130/270 (48%), Gaps = 18/270 (6%) Query: 24 EWGDPHNPNVLVCVHGVTRVSDDFDVMARDLCDTYRVVCPDVVGRGRSGRLGNPQHYAIP 83 EWGDP +L+ +HG+ + F +A+ L YR + D GRG+S +Y Sbjct: 19 EWGDPQGFPLLL-LHGIRGYGETFGDLAQRLPPGYRCIALDQRGRGQSDWDAGANYYT-D 76 Query: 84 QYVSDMVTLLARLDADSVDWFGTSMGGLIGMGLASLPDNPVRRLVLNDIGPSI--NGSAL 141 YV+D+ L+ARL+ D G SMGG+ + A+ VRRL++ D GP N Sbjct: 77 AYVADVEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIEDAGPGAFENSPGA 136 Query: 142 ARI-GEYIGQDVRFATFEEAALYIRTISASFGPHTDEEWHKLASDVLRQDEEGKWKRHYD 200 RI E FA+++EA+ ++R + S T+E + ++L+ +G + YD Sbjct: 137 TRIRRELATTPSHFASWDEASTFMRALRPSV---TEEARQQRLHNMLKPAAQGGYTWRYD 193 Query: 201 LRLAEPFKSMTPEAASIGEAMLWAAYDAIRCPTLLVRGAQSDLLLPEVAQAMTQRGPKAK 260 + ++P+ + + + L A+ CPTL+VRG +SD L E+A M Q + Sbjct: 194 -HVGISSTRLSPDLSRVVD--LRPHIMALMCPTLVVRGERSDYLSAEMAAEMRQLNARIL 250 Query: 261 LVELEGVGHAPTFMHAQQ----IQVAKDFL 286 + GH ++H Q +V + FL Sbjct: 251 TSTIADAGH---YIHDDQPEAFAEVVRQFL 277 >gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum seropedicae SmR1] Length = 300 Score = 61.6 bits (148), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 62/233 (26%), Positives = 105/233 (45%), Gaps = 25/233 (10%) Query: 23 KEWGDPHNPNVLVCVHGVTRVSDDFDVMARDLCDTYRVVCPDVVGRGRSGRLGNPQHYAI 82 + WGDP P + + +HG +S F M L + V+ PD G G + R Y Sbjct: 25 RHWGDPEAPKIFM-LHGWMDMSASFQFMVDALQGQWHVIAPDWRGFGLTER-SQADSYWF 82 Query: 83 PQYVSDMVTLLARL-DADSVDWFGTSMGGLIGMGLASLPDNPVRRLV-LNDIG-PSIN-G 138 P Y++D+ ++ +++++ G SMGG + A + ++RL+ L +G P+ Sbjct: 83 PDYLADLDAIIDHYAGSEAINLLGHSMGGNVVTLYAGVRPQRIKRLINLEGLGLPATQPE 142 Query: 139 SALARIGEYIGQDVR-------FATFEEAALYIRTISASFGPHTDEEWHKLASDVLRQDE 191 A R +++ +VR +AT EE A ++ +A +DE LA +QD+ Sbjct: 143 QAPGRFAQWM-DEVRNRPVLRGYATLEEVAQRLQRNNARL---SDERAAFLAQHWAQQDD 198 Query: 192 EGKWKRHYDLRLAEP-FKSMTPEAASIGEAMLWAAYDAIRCPTLLVRGAQSDL 243 G W+ L +P K ++P E M A + I P L + SD+ Sbjct: 199 SGLWQ-----ILGDPAHKQVSPILYRADEMM--ACWRRITAPVLWIEAIDSDI 244 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 969,219 Number of extensions: 42279 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 3 Length of query: 288 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 204 Effective length of database: 1,222,130 Effective search space: 249314520 Effective search space used: 249314520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)