BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0203.p (354 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase [Her... 191 9e-50 gi|300312796|ref|YP_003776888.1| 2-nitropropane dioxygenase [Her... 168 8e-43 gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase [Her... 58 1e-09 >gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 191 bits (485), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 132/286 (46%), Positives = 174/286 (60%), Gaps = 21/286 (7%) Query: 4 LQTLLGIELPLIQAPLAGIQGHAMAIAVSNAGGLGSLPCAMLTPETLRKELELIRAGTSK 63 L LG+ P+IQAP+AG MA A SNAG LGSL E R+ L + A T K Sbjct: 7 LMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLHALTEK 66 Query: 64 PYNVNFFAHTPPTPDAARE------XDPDTIPQGAGR--AP-------FSAEAA--ELLS 106 PYN+N F H P D+ R+ P + + GR AP F+A+ +++ Sbjct: 67 PYNINVFCHQPAVLDSERDRAWLAHLQP-LLDEFGGRIAAPLKEIYQSFNADTQMQQVVL 125 Query: 107 EFKPAVVSFHFGLPDQALLDRVRSWGAKVLCSATTVPEARWLEEQGVDAIIAQGIEAGGH 166 E PAVVSFHFGLP ++ +R G ++ +AT + EAR +E G+DAI+AQG EAGGH Sbjct: 126 EHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGH 185 Query: 167 RGFFL-THDLSTQIGTFALLPQIVRAVNVPVIAAGGIADAAGVAAAKSLGAAGVQIGTSY 225 RG F T D QIG+FALL +V+ +PVIAAGGI D G+AAA LGA VQ+GT++ Sbjct: 186 RGIFDPTKD--EQIGSFALLRLLVQHTRLPVIAAGGIMDGHGIAAALQLGAQAVQMGTAF 243 Query: 226 MLTPEASTSAPHRAALKGPAAEHTALTTLFTGGPARGIVNRVMREL 271 +LT E++ +A +RA LK P A+HT +T +G PARGIVNR M E+ Sbjct: 244 LLTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEI 289 >gi|300312796|ref|YP_003776888.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] Length = 358 Score = 168 bits (425), Expect = 8e-43, Method: Compositional matrix adjust. Identities = 125/313 (39%), Positives = 171/313 (54%), Gaps = 13/313 (4%) Query: 8 LGIELPLIQAPLAGIQGHAMAIAVSNAGGLGSLPCAMLTPETLRKELELIRAGTSKPYNV 67 GI+ P++Q P+ G M AVSNAGG+G+L A +T+R ++ IR+ T+KP+ V Sbjct: 13 FGIDFPIVQGPMNGGSTPEMVAAVSNAGGIGALAAANFDVQTMRTKVNAIRSLTTKPFCV 72 Query: 68 NFFA-HTP-PTP----DAAREXDPDT----IPQGAGRAPFSAEAAELLSEF---KPAVVS 114 N F TP PTP DA P +P A + E E L K V S Sbjct: 73 NLFVLETPAPTPGEIRDALDRLQPIRAELGLPPAAAPERYCEEFREQLDALIALKAPVAS 132 Query: 115 FHFGLPDQALLDRVRSWGAKVLCSATTVPEARWLEEQGVDAIIAQGIEAGGHRGFFLTHD 174 F FGL D A ++R+ + G+ V+ +AT V EA + G DAI AQG EAGGHRG F+ H Sbjct: 133 FTFGLLDAASIERLHAAGSLVIGTATNVAEALAWQANGADAICAQGAEAGGHRGTFIGHP 192 Query: 175 LSTQIGTFALLPQIVRAVNVPVIAAGGIADAAGVAAAKSLGAAGVQIGTSYMLTPEASTS 234 ++ IGT AL+PQIV AV +PVIAAGGI D G+AAA +LGA Q+GT++++T E+ Sbjct: 193 DASMIGTLALVPQIVDAVALPVIAAGGIMDGRGIAAALTLGAQAAQLGTAFLMTAESPIH 252 Query: 235 APHRAALKGPAAEHTALTTLFTGGPARGIVNRVMRELGPLSSAAQVFPLATAAIAPLRSK 294 A + ++ T T FTG PARGI+N M +G +P+ A +R Sbjct: 253 AAWQEKIRLSTDTSTRTTRTFTGRPARGILNDFMLRMGRYEQELPAYPVQHALTTEIRKA 312 Query: 295 AEAQGRGFFXVIF 307 A R F ++ Sbjct: 313 AGQANRPEFMSLW 325 >gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 68/265 (25%), Positives = 110/265 (41%), Gaps = 41/265 (15%) Query: 5 QTLLGIELPLIQAPLAGIQGHAMAIAVSNAGGLGSLPCAMLTP--------ETLRKELEL 56 + L + LP+I +P+ G A+ A AG +GS P P L++EL Sbjct: 9 KALQDLRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLTQLQEELAQ 68 Query: 57 IRAGTSK----PYNVNFFAHTPPTPDAAREXDPDTIPQGAGRAPFSAEAAELLSEFKPAV 112 +A ++ P VN H Q R + + A + P + Sbjct: 69 WQAEHAQRKVGPIAVNQIVH-----------------QSNDR--LAQDVATCVKHQVPII 109 Query: 113 VSFHFGLPDQALLDRVRSWGAKVLCSATTVPEARWLEEQGVDAIIAQGIEAGGHRGFFLT 172 +S P Q +LD V S+G VL ++ A E GVD +I AGG Sbjct: 110 IS-SLRAPPQEMLDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGG------- 161 Query: 173 HDLSTQIGTFALLPQIVRAVNVPVIAAGGIADAAGVAAAKSLGAAGVQIGTSYMLTPEAS 232 + + FAL+ ++ + P++ +G IA V AA+++GA +G+ ++ T E++ Sbjct: 162 --HAGTLSPFALVGEVRKMFKGPLVLSGAIATGDAVLAAQAMGADFAYMGSRWLATQESN 219 Query: 233 TSAPHRAALKGPAAEHTALTTLFTG 257 +R A+ A T LFTG Sbjct: 220 VDQAYRQAIVDSTAADVVYTNLFTG 244 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,134,017 Number of extensions: 46686 Number of successful extensions: 202 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 3 Length of query: 354 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 268 Effective length of database: 1,212,660 Effective search space: 324992880 Effective search space used: 324992880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)