BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0199.p (357 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 117 2e-27 gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 106 2e-24 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 88 1e-18 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 72 7e-14 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 68 1e-12 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 117 bits (292), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 94/346 (27%), Positives = 158/346 (45%), Gaps = 25/346 (7%) Query: 4 VVCHAPKDYRLQRVKRPAAGINEVIIKIASCGICAGDCKCYA----GAARFWGDKEKNKP 59 +V HAP D R+Q + A ++ +K+ + GIC D Y G R Sbjct: 5 LVIHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVR---------- 54 Query: 60 SYVKPPVIPGHEFWGHVVELGPGAETYHGVTLGDRVIAEQIVPCQKCRYCKTGYRWMCEV 119 +K P++ GHE G + E+G + G R+ PC C+YC+ G + C Sbjct: 55 --IKEPMVLGHEVSGQIAEVGTAVTD---IAPGTRIAISPSRPCGACKYCQEGKQNHCLD 109 Query: 120 HDVYGFQRQV--AEGGMADYMRLSARAVIHKIPTHIAFEDAAVTEPLSCAIHAVNRGDIQ 177 YG + +G + + R+ H + H++ +AA+ EPLS A+HAV R Sbjct: 110 MRFYGSAMRTPHVQGAFRQEIIIE-RSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPL 168 Query: 178 LDDVVVIAGAGTLGLLMVQVARLKTPKKLVVIDMIDERLELAKIFGADIVINPTRENTEK 237 + V++ G G +G L+V AR ++VV D+ L+ A GAD IN E Sbjct: 169 VGKRVLVTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAIN-MAETPAA 227 Query: 238 VVRSLTDNYGCDVYIETTGAPIGVTQGLDMIRKLGRFVEFSVFGRETTVDWSIIGEEKEL 297 + D DV E +G + L+ +R V+ + G ++G KEL Sbjct: 228 LKAYAADKGTFDVLFEASGNERALVGALEALRPQAIIVQVGLGGDIQFPANLLVG--KEL 285 Query: 298 DVRGAHLSADCYPIAIDLLARGLVTSKGIVTHSFALSEWDKAVKVA 343 + RGA + + +A++L+ R LV K ++T ++ +++ +A ++A Sbjct: 286 EWRGAFRFHEEFAMAVELMNRKLVDVKPLITATYPIAQAVEAFEIA 331 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 106 bits (265), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 91/347 (26%), Positives = 157/347 (45%), Gaps = 18/347 (5%) Query: 1 MLAVVCHAPKDYRLQRVKRPAA-GINEVIIKIASCGICAGDCKCYAGAARFWGDKEKNKP 59 M A+V A ++ +L+ + P G +V I+I + GIC D Y + P Sbjct: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGS--------IGP 52 Query: 60 SYVKPPVIPGHEFWGHVVELGPGAETYHGVTLGDRVIAEQIVPCQKCRYCKTGYRWMCEV 119 V+ P++ GHE G V+E+G + +GDRV E +P R M + Sbjct: 53 FKVEAPMVLGHEASGTVIEVG---SAVSHLKVGDRVCMEPGIPRLDS---PATLRGMYNL 106 Query: 120 HDVYGFQRQVAEGGMADYMRLSARAVIHKIPTHIAFEDAAVTEPLSCAIHAVNRGDIQLD 179 F G + A +++P +++F + A+ EPLS + A + ++ Sbjct: 107 DPAVRFWATPPIHGCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPG 166 Query: 180 DVVVIAGAGTLGLLMVQVARLKTPKKLVVIDMIDERLELAKIFGADIVINPTRENTEKVV 239 D V+ GAGT+G + A ++++ D++ E+L A I ++ TRE VV Sbjct: 167 DTAVVIGAGTIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVV 226 Query: 240 RSLTDNYGCDVYIETTGAPIGVTQG-LDMIRKLGRFVEFSVFGRETTVDWSIIGEEKELD 298 R TD +G DV E +G GV Q LD++ G V + +D + + KE+ Sbjct: 227 RQATDGWGADVVFEASGH-AGVYQTLLDLVCPGGCAVLVGMPPAPVALDV-VAMQTKEVR 284 Query: 299 VRGAHLSADCYPIAIDLLARGLVTSKGIVTHSFALSEWDKAVKVADS 345 + A+ +P A+ L++ G++ K ++ F S+ +A + A S Sbjct: 285 LESVFRYANIFPRALALISSGMIDVKPFISRKFPFSQSIRAFEEAAS 331 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 88.2 bits (217), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 86/314 (27%), Positives = 138/314 (43%), Gaps = 42/314 (13%) Query: 3 AVVCHAPKDYRLQRVKRPAAGINEVIIKIASCGICAGDCKCYAGAARFWGDKEKNKPSYV 62 AVV K +++V P ++++K + G+C D G + P Sbjct: 8 AVVREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHG----------DWPVKP 57 Query: 63 KPPVIPGHEFWGHVVELGPGAETYHGVTLGDRVIAEQI-VPCQKCRYCKTGYRWMCEVHD 121 PP IPGHE G+V +G G + V GDRV + C C C+TG+ +C Sbjct: 58 TPPFIPGHEGTGYVAAVGAGVKH---VKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQ 114 Query: 122 VYGFQRQVAEGGMADYMRLSARAVIHKIPTHIAFEDAAVTEPLSCAIHAVNRG----DIQ 177 G+ G A+Y + V H +P ++ F AA P+ CA V +G +++ Sbjct: 115 NTGYS---VNGSFAEYGLADPKFVGH-LPDNLDFGPAA---PVLCAGVTVYKGLKETEVR 167 Query: 178 LDDVVVIAGAGTLGLLMVQVARLKTPKKLVVIDMIDERLELAKIFGADIVINPTRENT-E 236 + VVI+G G LG + VQ A+ +V D+ +++L LAK GAD+ ++ N Sbjct: 168 PGEWVVISGIGGLGHMAVQYAKAMG-MHVVAADIHEDKLALAKKLGADLTVDGRNHNAVA 226 Query: 237 KVVRSLTDNYGCDVYIETTGAPIGVTQGLDMIRKLGRF---------VEFSVFG---RET 284 +V R + +G V T +P + Q +R G + VF + Sbjct: 227 EVQRIIGGAHGALV---TAVSPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRI 283 Query: 285 TVDWSIIGEEKELD 298 TV SI+G ++L+ Sbjct: 284 TVRGSIVGTRQDLE 297 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 72.0 bits (175), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 70/279 (25%), Positives = 116/279 (41%), Gaps = 38/279 (13%) Query: 1 MLAVVCHAPKDYRLQRVKRPA-AGINEVIIKIASCGICAGDCKCYAGAARFWGDKEKNKP 59 M A++ H+ D R++ V P ++VI+K+ + IC D Y G K Sbjct: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRG-----------KI 49 Query: 60 SYVKPPVIPGHEFWGHVVELGPGAETYHGVTLGDRVIAEQIVPCQKCRYCKTGYRWMCEV 119 K I GHEF G VVE GP + + GDRV+ ++ C +C +C CE Sbjct: 50 PATKAGDILGHEFMGVVVEAGPAIKR---LKKGDRVVVPFVIACGQCFFCDQHLYAACET 106 Query: 120 HD-----------------VYGFQRQVA--EGGMADYMRLSARAVIHKIPTHIAFEDAAV 160 + ++G+ GG A+Y+R+ +A + I A D V Sbjct: 107 TNPDRGALMNAKGIRSGAAMFGYSHLYGGMPGGQAEYVRVP-KADVGPIRIDSALADEQV 165 Query: 161 ---TEPLSCAIHAVNRGDIQLDDVVVIAGAGTLGLLMVQVARLKTPKKLVVIDMIDERLE 217 ++ L AV ++ V I GAG +G + AR+ +++ ++D RL Sbjct: 166 LFLSDILPTGYQAVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLR 225 Query: 218 LAKIFGADIVINPTRENTEKVVRSLTDNYGCDVYIETTG 256 A+ I +N + + + + + T G D I+ G Sbjct: 226 FAQEQYDVIPVNFDQVDPAEYIIANTAGRGVDGVIDAVG 264 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 77/315 (24%), Positives = 132/315 (41%), Gaps = 49/315 (15%) Query: 23 GINEVIIKIASCGICAGDCKCYAGAARFWGDKEKNKPSYVKPPVIPGHEFWGHVVELGPG 82 G EV++++ + G+C D G +P V P+ GHE VVE+G G Sbjct: 34 GPGEVLVRMKAAGLCHSDLSVITGV----------RPRPV--PMALGHEASAEVVEVGSG 81 Query: 83 AETYHGVTLGDRVIAEQIVPCQKCRYCKTGYRWMCE-------VHDVYGFQRQVAE---- 131 GD V+ + C C C G +CE V ++ QR++ + Sbjct: 82 VNDLRA---GDLVVLVFVPSCGHCLPCMEGRPALCEPGAATNTVGELLSGQRRLRDKTQP 138 Query: 132 -------GGMADYMRLSARAVIHKIPTHIAFEDAAVTEPLSCAI-----HAVNRGDIQLD 179 AD+ +S R+ + KI I AA+ CA+ AVN +++ Sbjct: 139 VHHHLGVSAFADHAVVSRRSCV-KIEADIDPVQAAL---FGCAVLTGVGAAVNTAEVKAG 194 Query: 180 DVVVIAGAGTLGLLMVQVARLKTPKKLVVIDMIDERLELAKIFGADIVINPTRENTEKVV 239 + G G +GL + A ++++ +D+ D +L LA+ GA +N + + V Sbjct: 195 TTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVEKV 254 Query: 240 RSLTDNYGCDVYIETTGAPIGVTQGLDMIRKLGRFVE--FSVFGRETTVDW-SIIGEEKE 296 R+LT G D E G+ + M R+ G V G+ + S++ EE+ Sbjct: 255 RALTRG-GVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVAEERT 313 Query: 297 LDVRGAHLSADCYPI 311 V+G+++ + C P+ Sbjct: 314 --VKGSYIGS-CVPV 325 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,106,887 Number of extensions: 45620 Number of successful extensions: 175 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 5 Length of query: 357 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 271 Effective length of database: 1,212,660 Effective search space: 328630860 Effective search space used: 328630860 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)