BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0181.p (980 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 271 3e-73 gi|300313029|ref|YP_003777121.1| two component hybrid sensor his... 231 2e-61 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 207 3e-54 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 182 1e-46 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 181 3e-46 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 271 bits (692), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 161/413 (38%), Positives = 222/413 (53%), Gaps = 56/413 (13%) Query: 397 ERANHAAAAKSSFLANMSHEIRTPMNSIIGFTELLLQSDLTPAQRDHLNTVRQSSRSLLR 456 E A+HA AKS FLA +SHEIRTPMN I+G +ELL++S L+ QRD+ +++ ++ L+R Sbjct: 339 EEADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMESGLSSKQRDYADSIHGATGGLVR 398 Query: 457 LINDILDTTKMEKGHLDLEETVFSLRAMSMQVESSLRLSAHAKNLTLTTHYGADTPDYFR 516 +INDILD +K+E G LDLE F + ++ R S K++ H D P+ Sbjct: 399 IINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDVQFDLHL--DCPENLS 456 Query: 517 --GDPLRLLQILTNLIGNAIKFTEQGGVDVLF-------GLENEMIHVQVRDTGIGMTQQ 567 GDP RL Q+L NL+ NAIKFT+ G V + G + + DTGIG++ + Sbjct: 457 VVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRWRLFFAITDTGIGISPE 516 Query: 568 QAESIFSPFTQADASISRRFGGTGLGTTIARQLAELMGGRIEVVSEPGRGSTFHVWLSLP 627 + +F PF QAD+SISRRFGGTGLG I++ L ELMGG I S PG + F + P Sbjct: 517 ALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMGGTIACQSIPGESTRFSFEILCP 576 Query: 628 QA-----------------------------------------NAPENTHPAKANCRALP 646 +A NAP+ A+ LP Sbjct: 577 EASTPAPEPVSEAAAPAPATALATTAPALPPAAEAPGTLMPPDNAPQPPSNVDASATVLP 636 Query: 647 AL----RILAADDVAQNLELLSLVLESQGHSVTLARDGAEAVQKFKEGSFDVALMDVHMP 702 +L RIL A+D N +LL ++L +G + +G +AV+ + G +D+ LMD MP Sbjct: 637 SLLAGARILMAEDTEINRQLLRILLARKGCILQEVENGLQAVEAIRTGHYDLVLMDCMMP 696 Query: 703 VIDGLQATQLIRQHERTLGLRRMPVIALTASVMTEDRRAALQAGMDGFAIKPL 755 ++DG QAT IR HE GL R P+IALTAS + DR+ L AGMD + KP Sbjct: 697 IMDGYQATAEIRAHEAARGLPRTPIIALTASAIEGDRQRCLDAGMDDYLSKPF 749 >gi|300313029|ref|YP_003777121.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1524 Score = 231 bits (589), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 158/420 (37%), Positives = 233/420 (55%), Gaps = 46/420 (10%) Query: 381 DISERHALEASLRETAERANHAAAAKSSFLANMSHEIRTPMNSIIGFTELLLQSDLTPAQ 440 DI++R E +L + +A A +AKS FLA++SHEIRTPMN ++G +LL ++ PAQ Sbjct: 872 DITQRIEFENNLVDARLKAEQANSAKSLFLASVSHEIRTPMNGVLGMIQLLQRTQPLPAQ 931 Query: 441 RDHLNTVRQSSRSLLRLINDILDTTKMEKGHLDLEETVFSLRAMSMQVESSLRLSAHAKN 500 +D+L+ S++SLL L+ND+LD +KM+ L L+ F L + + L S Sbjct: 932 QDYLDKAEISAKSLLNLLNDLLDFSKMDAKQLQLDLQAFELERFGHNLAAVLAGSPPQAG 991 Query: 501 LTLTTHYGADTPDYFRGDPLRLLQILTNLIGNAIKFTEQGGVDVLFGLENEM------IH 554 ++L P GD RL Q+L NL GNA+KFT QG +DV F + + + Sbjct: 992 VSLHIALDPQLPRRLVGDAHRLQQVLVNLTGNALKFTHQGHIDVHFTRLDPLPGRTVALR 1051 Query: 555 VQVRDTGIGMTQQQAESIFSPFTQADASISRRFGGTGLGTTIARQLAELMGGRIEVVSEP 614 V+V DTGIG+ + + IF FTQA+AS +RR+GGTGLG I++ L LMGG+++V SEP Sbjct: 1052 VEVSDTGIGIAADRLDDIFDAFTQAEASTTRRYGGTGLGLAISKHLVGLMGGQLQVSSEP 1111 Query: 615 GRGSTFHVWLSL--------------------PQ----------ANAPENTHPAKANCRA 644 GRGS F W L PQ + APE A + + Sbjct: 1112 GRGSRF--WFDLQLAVDDDSVLGPAAADDAARPQPLQAASHTAGSRAPETARSAP-DAQC 1168 Query: 645 LPALRILAADDVAQNLELLSLVLESQGHSVTLARDGAEAVQKFKEGS--FDVALMDVHMP 702 L ++IL +D N ++ S +L G VT A G+ V + + FD+ LMD+ MP Sbjct: 1169 LQGVQILLVEDNPVNRQVASELLALSGAQVTCADSGSMGVAMACDPARHFDLVLMDLQMP 1228 Query: 703 VIDGLQATQLIR-QHERTLGLRRMPVIALTASVMTEDRRAALQAGMDGFAIKPLDVPRLF 761 +DG++AT+ IR Q+E LR +P++A+TA+ ED++ ++AGM G KP+D+ L Sbjct: 1229 GMDGIEATRRIRGQYE----LRGLPILAMTANASPEDKQRCIEAGMAGHIAKPIDIATLI 1284 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 207 bits (528), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 129/387 (33%), Positives = 198/387 (51%), Gaps = 7/387 (1%) Query: 381 DISERHALEASLRETAERANHAAAAKSSFLANMSHEIRTPMNSIIGFTELL-LQSDLTPA 439 +I +A L+ E A A AKS F+A MSHE+RTP+NSI+G+ ++L + + + PA Sbjct: 663 EIEAHKQTDAELQRAKEAAESANLAKSRFVAGMSHELRTPLNSILGYAQILHVDATIPPA 722 Query: 440 QRDHLNTVRQSSRSLLRLINDILDTTKMEKGHLDLEETVFSLRAMSMQVESSLRLSAHAK 499 ++D + +R+S LL LI+ +LD K+E G + L +L A Q+ A K Sbjct: 723 RKDAIGVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGMFAPQAEQK 782 Query: 500 NLTLTTHYGADTPDYFRGDPLRLLQILTNLIGNAIKFTEQGGVDVLFGLENEMIHVQVRD 559 L P D RL QIL NL+GNA+KFT+ GGV + E+ + V D Sbjct: 783 GLHFRFDKSGRLPQIVHADQKRLRQILINLLGNAVKFTDHGGVTLRLRYAREIAYFDVID 842 Query: 560 TGIGMTQQQAESIFSPFTQADASISRRFGGTGLGTTIARQLAELMGGRIEVVSEPGRGST 619 TGIG+ E IF PF ++ A+ R GTGLG I+R L +MGG + V S+ G+GS+ Sbjct: 843 TGIGIADDDRERIFLPFERSSAANLREDIGTGLGLAISRMLVHIMGGELSVQSQVGQGSS 902 Query: 620 FHVWLSLPQANAPEN--THPAKANCRALPALRILAADDVAQNLELLSLVLESQGHSVTLA 677 FH+ + LP+ + P + + A P+ IL DD A LL +L G +V+ A Sbjct: 903 FHLKIYLPEVHVPRPRIKLEDQMSGYAGPSRHILVVDDQASQRRLLKDMLMPLGFTVSEA 962 Query: 678 RDGAEAVQKFKEGSFDVALMDVHMPVIDGLQATQLIRQHERTLGLRRMPVIALTASVMTE 737 G ++ D+ L+D+ MP +DG + IR GL ++P++ ++A+ Sbjct: 963 DSGLACMETLGHALPDLVLLDIAMPQMDGWSVARAIRAR----GLAQLPILLVSANAFEN 1018 Query: 738 DRRAALQAGMDGFAIKPLDVPRLFEEI 764 ++ F +KP+ L +I Sbjct: 1019 LHERNEPPLVNDFVVKPVSYHELLGKI 1045 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 182 bits (462), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 140/414 (33%), Positives = 198/414 (47%), Gaps = 69/414 (16%) Query: 397 ERANHAAAAKSSFLANMSHEIRTPMNSIIGFTELLL---QSDLTPAQRDHLNTVRQSSRS 453 ERAN KS FLANMSHE+RTP+NS + +LL Q +L+ Q T+ + Sbjct: 469 ERANQY---KSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRFAQTISSAGND 525 Query: 454 LLRLINDILDTTKMEKGHLDLEETVFSLRAMSMQVESSLRLSAHAKNLTLTTHYGADTPD 513 LL LINDILD +KME G LD+ F L ++ ++ +S ++A K L P Sbjct: 526 LLALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVEVEPGVPQ 585 Query: 514 YFRGDPLRLLQILTNLIGNAIKFTEQGGVDV-LFGLENEMIHVQVRDTGIGMTQQQAESI 572 D RL QIL NL+ NAIKFTE+G V + ++ I VRDTGIG+ + Q + I Sbjct: 586 RMETDIQRLGQILKNLLSNAIKFTEKGEVSLRIYPDAGGRIAFAVRDTGIGIGEDQHQFI 645 Query: 573 FSPFTQADASISRRFGGTGLGTTIARQLAELMGGRIEVVSEPGRGSTFHVWLSLPQ---- 628 F F QAD S R++GGTGLG +I+R LA L+GG I V S G GS F LSLP Sbjct: 646 FEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGEGSIFT--LSLPPLYSS 703 Query: 629 ---------------------------ANAPE---NTHPAKANCRALPALRILAADDVAQ 658 A AP+ N PA A + L + I D + Sbjct: 704 EQAVSNTAPEPTPAAAAAASTLQAAHVATAPDVRINRTPAAAAPQRLQEISIDDDRDRIE 763 Query: 659 NLELLSLVLESQGHSVTLARD-----------------GAEAVQKFKEGSFDVALMDVHM 701 L LV+E + RD G A + ++ + LMD+++ Sbjct: 764 TSSRLILVIEDDAAFAMILRDLAREMGFQCVVTHSANEGVAAAEMYRPSAI---LMDMNL 820 Query: 702 PVIDGLQATQLIRQHERTLGLRRMPVIALTASVMTEDRRAALQAGMDGFAIKPL 755 P GL I+++ +T R +PV ++ + + + A++ G G+A+KP+ Sbjct: 821 PDFSGLGVLDQIKRNPKT---RHIPVHVVSVADYSHE---AMERGAIGYALKPV 868 Score = 85.1 bits (209), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 48/119 (40%), Positives = 70/119 (58%), Gaps = 9/119 (7%) Query: 649 RILAADDVAQNLELLSLVLESQGHSVTLARDGAEAVQKFK------EGSFDVALMDVHMP 702 RIL +D +N+ LS VLE +G V +AR+G EA+Q + + D+ LMD+ MP Sbjct: 1036 RILVVEDDVRNIFALSSVLEPKGMKVEIARNGREALQALQRSQDKPDAPIDLVLMDIMMP 1095 Query: 703 VIDGLQATQLIRQHERTLGLRRMPVIALTASVMTEDRRAALQAGMDGFAIKPLDVPRLF 761 +DGL A + IR+ +R+P+IALTA M +D+ L AG + + KPLDV +L Sbjct: 1096 EMDGLTAMREIRKQA---VWKRLPIIALTAKAMKDDQEKCLAAGANDYIAKPLDVEKLL 1151 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 181 bits (458), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 97/249 (38%), Positives = 143/249 (57%), Gaps = 9/249 (3%) Query: 391 SLRETAERANHAAAAKSSFLANMSHEIRTPMNSIIGFTELLLQSDLTPAQRDHLNTVRQS 450 L + E A+HA + +FL+ MSHEIRTPMN+++G LL + L Q + + S Sbjct: 72 KLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLRSTRLDTQQAEFAKAIEDS 131 Query: 451 SRSLLRLINDILDTTKMEKGHLDLEETVFSLRAMSMQVESSLRLSAHAKNLTLTTHYGAD 510 + +LL ++ND+LD +K++ G +D+ F L + +L A K + L H + Sbjct: 132 AHALLGIVNDVLDFSKIDVGKMDIAVVEFDLAKVVEASAEALSFKAREKRVRLLAHLDSR 191 Query: 511 TPDYFRGDPLRLLQILTNLIGNAIKFTEQGGVDVLFGLE-------NEMIHVQVRDTGIG 563 P GDP R+ QIL NL+GNA+KFT G +VL ++ + + +VRD+GIG Sbjct: 192 LPPRLVGDPGRIRQILLNLLGNALKFTHAG--EVLLRVQALQVTSDHCRVRFEVRDSGIG 249 Query: 564 MTQQQAESIFSPFTQADASISRRFGGTGLGTTIARQLAELMGGRIEVVSEPGRGSTFHVW 623 M + +FSPF QAD+S++R++GGTGLG +I ++L ELMGG I V S G GS F Sbjct: 250 MDEDTLRRLFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVESALGTGSCFWFE 309 Query: 624 LSLPQANAP 632 L +P P Sbjct: 310 LGIPVTTGP 318 Score = 67.8 bits (164), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 63/231 (27%), Positives = 108/231 (46%), Gaps = 15/231 (6%) Query: 650 ILAADDVAQNLELLSLVLESQGHSVTLARDGAEAVQKFKEGSFDVALMDVHMPVIDGLQA 709 IL +D N ++ L + G++ +A +G EA+++ + + LMD MP++DG + Sbjct: 478 ILLVEDNVMNQKVAVYQLNAMGYAADIAANGLEALERLARHRYALVLMDCQMPLLDGYET 537 Query: 710 TQLIRQHERTLGLRRMPVIALTASVMTEDRRAALQAGMDGFAIKPLDVPRLFEEIAIVLD 769 T+ IR+ + L ++A+TA+ M DR L+AGMD + KPL +L +A L Sbjct: 538 TRRIRRMQGELA--HTTIVAMTANAMAGDRERCLEAGMDDYLTKPLLRSQLSAMLAHYLP 595 Query: 770 IPEQAEAPSRELPATPLASPVVDWNRGATLWGNEGRLMLSLQQLLETAAERYPLLAA-EA 828 + + P A ++D +R L+G + L+ + L PL+ +A Sbjct: 596 PSAASAG------SAPAAPQLIDEDRLQELFGQD--RALAHEMLAMFVEHTSPLMQELQA 647 Query: 829 EDIDWEASLLSL----HSLRGVAGNLALPVVSKLAGDMEAKMKSAQHAEAR 875 D E +L + H L G NL L + +L +ME + A+ R Sbjct: 648 HCQDQEPALDEVQALGHRLAGSCMNLGLDALGQLGREMEMAARQGDLAQTR 698 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,914,862 Number of extensions: 123047 Number of successful extensions: 757 Number of sequences better than 1.0e-08: 58 Number of HSP's gapped: 693 Number of HSP's successfully gapped: 61 Length of query: 980 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 886 Effective length of database: 1,174,780 Effective search space: 1040855080 Effective search space used: 1040855080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)