BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0168.p (599 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor p... 1151 0.0 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 167 3e-42 gi|300313617|ref|YP_003777709.1| translation elongation factor [... 100 3e-22 gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 ... 97 4e-21 gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspir... 89 8e-19 >gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 1151 bits (2977), Expect = 0.0, Method: Compositional matrix adjust. Identities = 556/599 (92%), Positives = 581/599 (96%), Gaps = 2/599 (0%) Query: 1 MNNIRNFSIIAHIDHGKSTLADRIIQLCGGLSNREMEAQVLDSMDIERERGITIKAQTAA 60 MNNIRNFSIIAHIDHGKSTLADRIIQLCGGLSNREMEAQVLDSMDIERERGITIKAQTAA Sbjct: 1 MNNIRNFSIIAHIDHGKSTLADRIIQLCGGLSNREMEAQVLDSMDIERERGITIKAQTAA 60 Query: 61 LHYKALDGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAL 120 L YKA DGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAL Sbjct: 61 LSYKAKDGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAL 120 Query: 121 ELGVEVVPVLNKIDLPSADPDNAKSEIEEVIGIDASDATPCSAKTGLGVQDVIEAMIAKV 180 +LGVEVVPVLNKIDLPSADPDNAKSEIE+VIGIDASDATPCSAKTGLGV+DV+EA++AKV Sbjct: 121 DLGVEVVPVLNKIDLPSADPDNAKSEIEDVIGIDASDATPCSAKTGLGVEDVLEAIVAKV 180 Query: 181 PAPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKEKIFLMATESTHLTESIGVF 240 PAPKGDP+APLQALIIDSWFDNYVGVVMLVRIVNGTLRPK+KI LMATE+ +LTESIGVF Sbjct: 181 PAPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKILLMATETQYLTESIGVF 240 Query: 241 TPKSQSRETLSAGQVGFIIAGIKELKAAKVGDTVTLAANKASEPLPGFKEVQPQVFAGLF 300 TPKSQSRE L+AGQVGF+IAGIKELK+A+VGDT+TLA+ A PLPGFKEVQPQVFAGLF Sbjct: 241 TPKSQSREALTAGQVGFVIAGIKELKSARVGDTITLASKPAPAPLPGFKEVQPQVFAGLF 300 Query: 301 PVEANQYDALRDSLEKLKLNDAALQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD 360 PVEANQYDALRDSLEKLKLNDAAL YEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD Sbjct: 301 PVEANQYDALRDSLEKLKLNDAALMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD 360 Query: 361 MDLITTAPTVVYEVVMRDGAGTILMVDNPSKMPEPSRIEEVREPIVTVNLYMPQEYVGSV 420 MDLITTAPTVVYEV RD GT+LMVDNPSKMPE S+IEE+REPIVTVNLYMPQEYVGSV Sbjct: 361 MDLITTAPTVVYEVQQRD--GTLLMVDNPSKMPEVSKIEEIREPIVTVNLYMPQEYVGSV 418 Query: 421 ITLCTQKRGVQINMSYHGKQVQLTYEMPMAEIVLDFFDRLKSTSRGYASMDYEFKEYRSA 480 ITLCTQKRG+Q+NMSYHGKQVQLTYEMPMAEIVLDFFDRLKSTSRGYASMDYEFKEYR+A Sbjct: 419 ITLCTQKRGIQMNMSYHGKQVQLTYEMPMAEIVLDFFDRLKSTSRGYASMDYEFKEYRAA 478 Query: 481 DVVKVDMLINSEKVDALAIIVHRANSQFRGRAVAAKMRELIPRQMFDVAIQAAIGATIIS 540 DVVKVDMLINSEKVDALAIIVHRA +Q RGR VAAKMRELIPRQMFDVAIQAAIGATIIS Sbjct: 479 DVVKVDMLINSEKVDALAIIVHRAAAQIRGRQVAAKMRELIPRQMFDVAIQAAIGATIIS 538 Query: 541 RENVKALRKNVLAKCYGGDISRKRKLLEKQKAGKKRMKQVGSVEIPQEAFLAILQVDDK 599 RENVKA+RKNVLAKCYGGDISRKRKLLEKQKAGKKRMKQVGSVEIPQEAFLAILQV+DK Sbjct: 539 RENVKAMRKNVLAKCYGGDISRKRKLLEKQKAGKKRMKQVGSVEIPQEAFLAILQVEDK 597 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 167 bits (422), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 149/509 (29%), Positives = 240/509 (47%), Gaps = 70/509 (13%) Query: 12 HIDHGKSTLADRIIQLCGGL-SNREMEAQVLDSMDIERERGITIKAQTAALHYKALDGQI 70 H+DHGK+TL D++++ G N++++ +V+DS DIE+ERGITI ++ A+ YK Sbjct: 30 HVDHGKTTLVDQLLRQSGTFRDNQQVDNRVMDSNDIEKERGITILSKNCAVEYKGT---- 85 Query: 71 YNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTALELGVEVVPVL 130 ++N++DTPGH DF EV R LS + LL+VDA +G QT AL LG++ + VL Sbjct: 86 -HINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGLKPIVVL 144 Query: 131 NKIDLPSADPD---NAKSEIEEVIGIDASDAT-PCSAKTGL-------------GVQDVI 173 NKID P A + NA E+ + +G P +GL ++ + Sbjct: 145 NKIDRPGARAEWAINATFELFDKLGATEEQLDFPVIYASGLNGYASLDPEAREGNMEPLF 204 Query: 174 EAMIAKVPAPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKEKIFLMATESTHL 233 +A++ VPA K DPDAPLQ I + +YVG + + RI+ G ++ + + M Sbjct: 205 DAILKYVPARKDDPDAPLQLQITSLEYSSYVGKIGVGRILAGRVKGGQDVVWMNGPDDKP 264 Query: 234 TESI--GVFTPKSQSRETLSAGQVG--FIIAGIKELKAAKVGDTVTLAANKASEPLPGFK 289 T++ V T K R + G +I GI+E +G T+ + A LP K Sbjct: 265 TKARINQVLTFKGLERVLVDEALAGDIVLINGIEE-----IGIGSTICSPDAPNGLPMLK 319 Query: 290 EVQPQVFAGLF----PVEANQ-----YDALRDSLEK-LKLNDAALQYEPEVSQALGFGFR 339 +P + P+ + +RD LEK LK N A + E + + Sbjct: 320 VDEPTLTMNFMVNTSPLAGREGKFVTTRQIRDRLEKELKSNMALRVVQAENDDST---YE 376 Query: 340 CGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVMRDGAGTILMVDNPSKMPEPSRIE 399 G LH+ I+ E + RE +L + P VV+ +V + + Sbjct: 377 VSGRGELHLTILIENMRRE-GFELAVSRPRVVFRMV--------------------NGVR 415 Query: 400 EVREPIVTVNLYMPQEYVGSVITLCTQKRGVQINMSYHGK-QVQLTYEMPMAEIVLDFFD 458 E EP + + + + + G V+ ++RG NM GK +V+L Y +P A ++ F Sbjct: 416 E--EPYENLTVDVEETHQGGVMEELGRRRGDLQNMEPDGKGRVRLEYHIP-ARGLIGFQG 472 Query: 459 RLKSTSRGYASMDYEFKEYRSADVVKVDM 487 + +RG M + F +Y+ D K ++ Sbjct: 473 EFMTLTRGTGLMSHVFDDYKPVDTTKGEL 501 >gi|300313617|ref|YP_003777709.1| translation elongation factor [Herbaspirillum seropedicae SmR1] Length = 701 Score = 100 bits (249), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 58/155 (37%), Positives = 89/155 (57%), Gaps = 7/155 (4%) Query: 1 MNNIRNFSIIAHIDHGKSTLADRIIQLCGGLSNREME----AQVLDSMDIERERGITIKA 56 + N RN I AHID GK+T +RI+ G++++ E A +D M+ E+ERGITI + Sbjct: 7 IENYRNIGISAHIDAGKTTTTERIL-FYTGVNHKIGEVHNGAATMDWMEQEQERGITITS 65 Query: 57 QTAALHYKALDGQI--YNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVA 114 +K + G + +N+IDTPGHVDF+ EV RS+ +GA++V D+ GV+ Q+ Sbjct: 66 AATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQPQSET 125 Query: 115 NCYTALELGVEVVPVLNKIDLPSADPDNAKSEIEE 149 A + V + +NK+D AD + +IEE Sbjct: 126 VWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEE 160 >gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 [Herbaspirillum seropedicae SmR1] Length = 530 Score = 96.7 bits (239), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 57/171 (33%), Positives = 92/171 (53%), Gaps = 15/171 (8%) Query: 5 RNFSIIAHIDHGKSTLADRI------IQLCGGLSNREMEAQVL-DSMDIERERGITIKAQ 57 R F II+H D GK+TL +++ IQL G + R+ D M+IE++RGI++ + Sbjct: 10 RTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEKQRGISVASS 69 Query: 58 TAALHYKALDGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCY 117 Y+ + +NL+DTPGH DFS + R L+A + AL+V+DA++GVE QT+ Sbjct: 70 VMQFEYRD-----HVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTIKLLN 124 Query: 118 TALELGVEVVPVLNKIDLPSADPDNAKSEIEEVIGIDASDATPCSAKTGLG 168 ++ +NK+D + D E+E V+ I + P + G+G Sbjct: 125 VCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCA---PVTWPIGMG 172 >gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspirillum seropedicae SmR1] Length = 701 Score = 89.4 bits (220), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 51/141 (36%), Positives = 81/141 (57%), Gaps = 7/141 (4%) Query: 5 RNFSIIAHIDHGKSTLADRIIQLCGGLSNREME----AQVLDSMDIERERGITIKAQTAA 60 RN I AHID GK+T +R++ G++++ E A +D M+ E+ERGITI + Sbjct: 11 RNIGISAHIDAGKTTTTERVL-FYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATT 69 Query: 61 LHYKALDGQI--YNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYT 118 ++K + +++N+IDTPGHVDF+ EV RS+ +GA +V A GV+ Q+ Sbjct: 70 CYWKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQ 129 Query: 119 ALELGVEVVPVLNKIDLPSAD 139 A + V + +NK+D A+ Sbjct: 130 ANKYKVPRLAFVNKMDRTGAN 150 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,669,392 Number of extensions: 66556 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 8 Length of query: 599 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 508 Effective length of database: 1,188,985 Effective search space: 604004380 Effective search space used: 604004380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)