BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0166.p (525 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 723 0.0 gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 368 e-103 gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 221 1e-58 gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 92 1e-19 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 723 bits (1866), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/473 (79%), Positives = 422/473 (89%), Gaps = 6/473 (1%) Query: 2 MIPAKKILLSSLLSASIFALSPALTGLSAAVAAPAVAGLPDFADIVDRTGPGVVNIRTTE 61 M P K L S+LL+AS+F + + + VA+PAVA LPDFADIV+RTGPGVVNIRTTE Sbjct: 1 MTPLKTTLASALLAASVFVAA-PVALAAPPVASPAVAALPDFADIVERTGPGVVNIRTTE 59 Query: 62 RVK-TGQPQAGADDEEMQEFFRRFFGVPMPRQQQPNTPNTPRPNPNRKGGSAQQEEVPRG 120 RV+ Q Q G DD EMQEFFRRFFG+P+PRQQQP TP ++GG QQEEVPRG Sbjct: 60 RVRQNAQGQGGVDDPEMQEFFRRFFGIPIPRQQQPGTPRGN----GKQGGQGQQEEVPRG 115 Query: 121 VGSGFIISADGYIMTNAHVVEGASDVYVTLTDKREFKAKIIGSDTRTDVALLKIDGTSLP 180 VGSGFIISADG+IMTNAHVVEGAS+VYVTLTDKREFKAKI+GSDTRTDVA+LKIDG++LP Sbjct: 116 VGSGFIISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLP 175 Query: 181 RLVIGDSDKIRAGEWVLAIGSPFGLENTVTAGIISAKARDTGDYLPLIQTDVAVNPGNSG 240 RL +GDSDKIR GEWVLAIGSPFGLENTVTAGI+SAKARDTGDYLPLIQTDVAVNPGNSG Sbjct: 176 RLNMGDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNSG 235 Query: 241 GPLINLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVSDQLKASGRVTRGRIGVQIGE 300 GPLINLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRV+DQLKASGRVTRGRIGVQIGE Sbjct: 236 GPLINLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVADQLKASGRVTRGRIGVQIGE 295 Query: 301 VTKDVAESLGLAKAQGALVQRIEPGGPAEKGGLEAGDIILKFNGVAIEKPSDLPRMVGGT 360 VTKDVAESLGLA+AQGALVQR+E GGPAEK GLEAGDIILK+NG AIE+PSDLPRMVG T Sbjct: 296 VTKDVAESLGLARAQGALVQRVEAGGPAEKAGLEAGDIILKYNGAAIERPSDLPRMVGST 355 Query: 361 KPGSRATVNIWRKGSARDIAVTVVEIEADKPAKVEEKKPKQEAAPNSLGLVVSDLSDAQK 420 KPG++ATV+IWRKGSARD++VTVVE+EADKP + EEKK K + APNSLGLVVSDLSDAQ+ Sbjct: 356 KPGAKATVSIWRKGSARDVSVTVVELEADKPKQAEEKKAKPDNAPNSLGLVVSDLSDAQR 415 Query: 421 KELKVDSGVLVESAVGVAAAAGLRAGDVVQRLNDVDIKDSRQFNGLVAKLDPK 473 KELKVD+GVLVE+ G AAAAG+R GDV+QRLN+VD+KD++QF LV+KLD K Sbjct: 416 KELKVDNGVLVEAVSGAAAAAGIRPGDVIQRLNEVDVKDAKQFAQLVSKLDAK 468 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 368 bits (945), Expect = e-103, Method: Compositional matrix adjust. Identities = 206/435 (47%), Positives = 278/435 (63%), Gaps = 40/435 (9%) Query: 42 DFADIVDRTGPGVVNIRTTERVKTGQPQAGADDEE--------MQEFFRRFFGVPMPRQQ 93 DF+ IV R GP VVNI T G+ + ADD++ EFF+RF P+ Q Sbjct: 64 DFSGIVQRYGPAVVNISVT-----GKARKVADDDDNGLDPNDPFSEFFKRF----GPQLQ 114 Query: 94 QPNTPNTPRPNPNRKGGSAQQEEVPRGVGSGFIISADGYIMTNAHVVEGASDVYVTLTDK 153 P QQ + G+GSGFIIS DG I+TNAHVV+GA +V V LTD+ Sbjct: 115 MP-----------------QQPRIMHGLGSGFIISPDGLILTNAHVVDGAQEVVVKLTDR 157 Query: 154 REFKAKIIGSDTRTDVALLKIDGTSLPRLVIGDSDKIRAGEWVLAIGSPFGLENTVTAGI 213 REFKAK++G D ++D+A+++ID +LP + IGD +++ G+ VLAIGSP+G +NT TAGI Sbjct: 158 REFKAKVLGIDKQSDIAVIRIDAKNLPTVQIGDPSRVKVGQPVLAIGSPYGFDNTATAGI 217 Query: 214 ISAKARDTGD--YLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYSRSGGFMGISFAV 271 ISAK+R D Y+P IQTDVAVNPGNSGGPL +L G+VIGINSQIYS++GGF G+SF++ Sbjct: 218 ISAKSRSLPDDNYVPFIQTDVAVNPGNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSI 277 Query: 272 PIDEALRVSDQLKASGRVTRGRIGVQIGEVTKDVAESLGLAKAQGALVQRIEPGGPAEKG 331 PID A++V QL G+VTRGR+GV + ++ + +++S GL KA+GALV +E G PA+K Sbjct: 278 PIDVAMKVEQQLVTHGKVTRGRLGVSVQDLNQALSDSFGLKKAEGALVSSVEKGSPADKA 337 Query: 332 GLEAGDIILKFNGVAIEKPSDLPRMVGGTKPGSRATVNIWRKGSARDIAVTVVEIEADKP 391 GL+AGD+I+ FNG AI+ DLP +V T PGS + + R G R I VTV E+ K Sbjct: 338 GLQAGDVIISFNGHAIDHSVDLPTLVADTPPGSTKPMEVMRAGKVRTINVTVGEM---KQ 394 Query: 392 AKVEEKKPKQEAAPNSLGLVVSDLSDAQKKELKVDSGVLVESAVGVAAAAGLRAGDVVQR 451 AK + K K + LGL V L ++ +L G+LVE G AA AG+++GDV+ Sbjct: 395 AKNDAKASKSDDQ-GRLGLAVRPLDKDEQAQLGGQKGLLVEDVSGPAAVAGIQSGDVILA 453 Query: 452 LNDVDIKDSRQFNGL 466 +N + Q L Sbjct: 454 VNGTPVSSVAQLRKL 468 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 221 bits (563), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 131/364 (35%), Positives = 200/364 (54%), Gaps = 28/364 (7%) Query: 23 PALTGLSAAVAAPAVAGLPDFADIVDRTGPGVVNIRTTERVKTGQPQAGADDEEMQEFFR 82 PA + + A P L + + P VVNI TT + PQ + FFR Sbjct: 40 PAQSTVQMQEATPNAPALDSYRHAARQAMPSVVNIFTTTEAR---PQKSPFQND--PFFR 94 Query: 83 RFFGVPMPRQQQPNTPNTPRPNPNRKGGSAQQEEVPRGVGSGFIISADGYIMTNAHVVEG 142 +FFG + QQ++ +GSG I+S GYI+TN HVVE Sbjct: 95 KFFG--------------------DQFDEQQQDDKQSSLGSGVIVSPQGYILTNNHVVEA 134 Query: 143 ASDVYVTLTDKREFKAKIIGSDTRTDVALLKIDGTSLPRLVIGDSDKIRAGEWVLAIGSP 202 A + V L D R+ AK++G D TD+A++KID +LP + +G + G+ VLAIG+P Sbjct: 135 ADKIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPAITLGHPENSSVGDVVLAIGNP 194 Query: 203 FGLENTVTAGIISAKARD---TGDYLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYS 259 FG+ TVT GI+SA R+ + IQTD A+NPGNSGG L++ G ++GIN+ IYS Sbjct: 195 FGVGQTVTMGIVSALGRNHLGINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYS 254 Query: 260 RSGGFMGISFAVPIDEALRVSDQLKASGRVTRGRIGVQIGEVTKDVAESLGLAKAQGALV 319 R+GG +GI FA+P+ A V + + + G+V RG IGV+ ++T ++AES GL K GA++ Sbjct: 255 RTGGNLGIGFAIPMSTAKTVMEAIISHGQVVRGWIGVEPQDITPELAESFGLGKKTGAII 314 Query: 320 QRIEPGGPAEKGGLEAGDIILKFNGVAIEKPSDLPRMVGGTKPGSRATVNIWRKGSARDI 379 + GGPA++ G+ GDI++ + + +++ ++ PG + + + RK + Sbjct: 315 AGVLKGGPADRAGMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQIAMTVLRKSQETKL 374 Query: 380 AVTV 383 +TV Sbjct: 375 NITV 378 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 91.7 bits (226), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 63/167 (37%), Positives = 97/167 (58%), Gaps = 9/167 (5%) Query: 122 GSGFIISADGYIMTNAHVVEGASDVYVTLTDKREFKAKIIGSDTRTDVALLKIDGTSLPR 181 G+GF I G++++N HVV A V V L RE A+++ D DVALLK + + Sbjct: 271 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 330 Query: 182 LVIGDSDKIRAGEWVLAIGSPFG--LENTVTAGIISAKARDTGDYLPLIQTDVAVNPGNS 239 L + D+ GE V A+GSP G T+T GI+S G +Q+DVA+ PG+S Sbjct: 331 LPL-RLDEPGLGEDVYALGSPLGDTFNTTLTRGILSGVRLLNGQE--FLQSDVAILPGSS 387 Query: 240 GGPLINLKGEVIGIN-SQIYSRSGGFMGISFAVPIDEAL-RVSDQLK 284 GGPL++ G+V+GI + + +R G G++F +P+ A+ R++ QL+ Sbjct: 388 GGPLLDRSGQVVGITVAGLGAR--GMAGMNFFIPLASAVERLNLQLQ 432 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,440,009 Number of extensions: 61253 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 4 Length of query: 525 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 435 Effective length of database: 1,193,720 Effective search space: 519268200 Effective search space used: 519268200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)