BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0159.p (257 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 414 e-117 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 174 8e-45 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 171 7e-44 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 157 6e-40 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 149 2e-37 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 414 bits (1065), Expect = e-117, Method: Compositional matrix adjust. Identities = 208/247 (84%), Positives = 223/247 (90%) Query: 10 QNLQNQTALVTGASRGIGRAIALELVRQGAKVVGTATSAAGAAAISEYLEQAQPGCGKGV 69 QNLQ Q ALVTGASRGIGRAIA EL RQGAKV+GTATS +GAAAI+EYL P G+G Sbjct: 4 QNLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGA 63 Query: 70 VLNVNDAAACSAVIDDVQKEFGGLSILVNNAGITQDQLAMRMKDEEWDNVIATNLTAVGR 129 VLNVNDA A A+++ VQK+FG LSILVNNAGITQDQLAMRMKDEEWD+VIATNLTAVGR Sbjct: 64 VLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGR 123 Query: 130 LSRAVLRGMMKARNGRIINITSVVGSAGNSGQMNYAAAKAGVAGMSRALAREIGSRNITV 189 LSRAVLRGMMKAR GRIINITSVVG AGN GQMNYAAAKAGVAGMSRALAREIGSRNITV Sbjct: 124 LSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITV 183 Query: 190 NCIAPGFIDTDMTKVLGEQQTAALLQQIPLGRLGAPEDIAAAVAFLASPQAAYITGTTLH 249 NC+APGFIDTDMT+ L EQQ+AA+LQQIPLGRLGA E+IA AVAFLASPQAAYITGTTL+ Sbjct: 184 NCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLN 243 Query: 250 VNGGMYM 256 VNGGMYM Sbjct: 244 VNGGMYM 250 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 174 bits (440), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 95/250 (38%), Positives = 148/250 (59%), Gaps = 10/250 (4%) Query: 12 LQNQTALVTGASRGIGRAIALELVRQGAKVVGTATSAAGAAAISEYLEQAQPGCGKGVVL 71 + + A VTG GIG AI L + G VV A + ++L + +G + Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVV--AGCGPNSTRKDKWLATMRE---QGFDI 55 Query: 72 -----NVNDAAACSAVIDDVQKEFGGLSILVNNAGITQDQLAMRMKDEEWDNVIATNLTA 126 NV++ + A + V+ E G + +LVNNAGIT+D +M ++WD VI TNL + Sbjct: 56 HASEGNVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNS 115 Query: 127 VGRLSRAVLRGMMKARNGRIINITSVVGSAGNSGQMNYAAAKAGVAGMSRALAREIGSRN 186 + +++ V+ GM + GRIINI+SV G G GQ NY+ AKAG+ G + ALA+E+ ++ Sbjct: 116 LFNVTKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKG 175 Query: 187 ITVNCIAPGFIDTDMTKVLGEQQTAALLQQIPLGRLGAPEDIAAAVAFLASPQAAYITGT 246 +TVN ++PG+I TDM + + + ++ IP+ RLG PE+IA+ VA++AS + Y TG+ Sbjct: 176 VTVNTVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGS 235 Query: 247 TLHVNGGMYM 256 +NGG+YM Sbjct: 236 DFSLNGGLYM 245 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 171 bits (432), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 107/245 (43%), Positives = 147/245 (60%), Gaps = 12/245 (4%) Query: 15 QTALVTGASRGIGRAIALELVRQGAKVVGTATSAAGAAAISEYLEQAQPGCGKGVVLNVN 74 + VTG+SRGIGRAIAL L R+G +V + AA + + G+ V + Sbjct: 7 HSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAA---DQVAAEIAALGRQVRVLQF 63 Query: 75 DAA----ACSAVIDDVQKEFGGLSILVNNAGITQDQLAMRMKDEEWDNVIATNLTAVGRL 130 D A A ++ DV++ G +V NAGIT+D M E+WD+V+ TNL + Sbjct: 64 DVANREQAAQVLLADVEQH-GCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNV 122 Query: 131 SRAVLRGMMKARN-GRIINITSVVGSAGNSGQMNYAAAKAGVAGMSRALAREIGSRNITV 189 ++ M++ R GRI+ + SV G GN GQ+NY+AAKAGV G ++ALA E+ R+ITV Sbjct: 123 LNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITV 182 Query: 190 NCIAPGFIDTDMTKVLGEQQTAALLQQIPLGRLGAPEDIAAAVAFLASPQAAYITGTTLH 249 NC+APG IDTDMT+ + +L+ IP R G PE++AAAVAFL SP AAYIT + Sbjct: 183 NCVAPGLIDTDMTE---DAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVIS 239 Query: 250 VNGGM 254 VNGG+ Sbjct: 240 VNGGL 244 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 157 bits (398), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 100/251 (39%), Positives = 147/251 (58%), Gaps = 9/251 (3%) Query: 12 LQNQTALVTGAS--RGIGRAIALELVRQGAKVVGTATSAAGAAAISEYLEQAQPGCGKGV 69 L+++ A++TG + G+G + A + QGA++V A A + L G+ Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQH----LGL 58 Query: 70 VLNVNDAAACSAVIDDVQKEFGGLSILVNNAGITQDQLAMRMKDEEWDNVIATNLTAVGR 129 V +V D AAC A V K+FG + ILVNNAG+TQ + + +++D ++ +L Sbjct: 59 VADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLY 118 Query: 130 LSRAVLRGMMKARNGRIINITSVVGSAGNS--GQMNYAAAKAGVAGMSRALAREIGSRNI 187 +S+AVL M ++G I+ I+SV G G +Y+AAKAGV G++RA+ARE G + I Sbjct: 119 MSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGI 178 Query: 188 TVNCIAPGFIDTDMTK-VLGEQQTAALLQQIPLGRLGAPEDIAAAVAFLASPQAAYITGT 246 VNCI PG I TD+ K +L ++ A + Q IPLGRLG P+D+ FLAS + Y TG Sbjct: 179 RVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGV 238 Query: 247 TLHVNGGMYMN 257 TL VNGGM ++ Sbjct: 239 TLDVNGGMLIH 249 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 149 bits (377), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 99/251 (39%), Positives = 143/251 (56%), Gaps = 9/251 (3%) Query: 12 LQNQTALVTGAS--RGIGRAIALELVRQGAKVVGTATSAAGAAAISEYLEQAQPGCGKGV 69 L+++ A++TG + G+G A A + QGA+VV + A AA + + G+ Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQH----LGL 58 Query: 70 VLNVNDAAACSAVIDDVQKEFGGLSILVNNAGITQDQLAMRMKDEEWDNVIATNLTAVGR 129 V +V D A+C + ++FG + ILVNNAGITQ + + ++D ++ +L Sbjct: 59 VADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLY 118 Query: 130 LSRAVLRGMMKARNGRIINITSVVGSAGNS--GQMNYAAAKAGVAGMSRALAREIGSRNI 187 +S+AVL M +NG II I+SV G G +Y+AAKAGV G++RA+ARE G I Sbjct: 119 MSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGI 178 Query: 188 TVNCIAPGFIDTDMTK-VLGEQQTAALLQQIPLGRLGAPEDIAAAVAFLASPQAAYITGT 246 VN I PG I TD+ K L E++ A + + IPL RLG +DI A FL S + Y TG Sbjct: 179 RVNSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGI 238 Query: 247 TLHVNGGMYMN 257 TL VNGGM ++ Sbjct: 239 TLDVNGGMLIH 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 690,881 Number of extensions: 27014 Number of successful extensions: 289 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 48 Length of query: 257 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 174 Effective length of database: 1,226,865 Effective search space: 213474510 Effective search space used: 213474510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)