BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0141.p (609 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311236|ref|YP_003775328.1| hypothetical protein Hsero_1911... 1056 0.0 gi|300313035|ref|YP_003777127.1| serine phosphatase, regulator o... 101 2e-22 gi|300309883|ref|YP_003773975.1| GGDEF domain/EAL domain signal ... 98 2e-21 gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709... 93 5e-20 gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 86 1e-17 >gi|300311236|ref|YP_003775328.1| hypothetical protein Hsero_1911 [Herbaspirillum seropedicae SmR1] Length = 600 Score = 1056 bits (2732), Expect = 0.0, Method: Compositional matrix adjust. Identities = 496/595 (83%), Positives = 549/595 (92%), Gaps = 2/595 (0%) Query: 15 ESQLLSHLHEIIAQRSLTALFQPIIRMRSGEIVGYEGLIRGPSNSPLHSPLNLFKVARAX 74 E +LS LHEIIAQR LTALFQPI+RMR+GEI+GYEGLIRGPSN+PLHSPL LFKVARA Sbjct: 5 EDPMLSLLHEIIAQRKLTALFQPIVRMRNGEILGYEGLIRGPSNTPLHSPLALFKVARA- 63 Query: 75 PNKLSVQVEHLCRHIVMAQFAQSGLPGNLFLNVSPGALVQPEAIQGETLRHLEKLGINPQ 134 + +SVQ+E+LCRHIV+ +FAQ GLPGNLFLNVSPGAL+QPEAIQGETLR+L+ LG+NPQ Sbjct: 64 -HNMSVQMEYLCRHIVLTRFAQLGLPGNLFLNVSPGALMQPEAIQGETLRYLDTLGVNPQ 122 Query: 135 RIIIELTENEPTYDYTLLRNAAMHYRNMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMH 194 RIIIELTENEPTYDY LRNAAMHYR+MGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMH Sbjct: 123 RIIIELTENEPTYDYAQLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMH 182 Query: 195 FIQGINNDPMKLQFVRSIQEIAEKSGSIVIAEGIETQAELLTIRDLGIACGQGYHIARPH 254 F+QGIN+DPMKLQFVRSIQEIAEKSGSIVIAEGIE+QAELLTIRDLGIACGQGYHI+RP Sbjct: 183 FVQGINHDPMKLQFVRSIQEIAEKSGSIVIAEGIESQAELLTIRDLGIACGQGYHISRPL 242 Query: 255 ANPKTVVGDDISKVLRRDKVPLAAQKYGPGKKSTTVFKLLRESLYVAPDVSNDKVYNLFV 314 P + DD++K LRRDKVPL A+KYGPGK STTVFKLLRE+ YV+P+VSND VY++FV Sbjct: 243 PEPPVSISDDVTKTLRRDKVPLDAKKYGPGKHSTTVFKLLRETRYVSPEVSNDTVYDMFV 302 Query: 315 NDPQLQALPVVSNGIPVGLINRYSMVERFARLYGRELYGKKSCTFFMDPNPLLTEQDTSL 374 NDPQLQALPVVSNG+P+GLINRYSMVERFARLYGRELYGKKSC FFMDPNP+LTEQ TSL Sbjct: 303 NDPQLQALPVVSNGLPIGLINRYSMVERFARLYGRELYGKKSCAFFMDPNPILTEQSTSL 362 Query: 375 QDLSHILVQAESHHLSNGFIIVDQGQYVGMGTGHDLLREITQMQIEAARYANPLTQLPGN 434 QDLS++LV AESHHLSNGFII D+GQY+G+GTGHDLLREITQMQI+AAR+ANPLTQLPGN Sbjct: 363 QDLSNLLVHAESHHLSNGFIITDRGQYMGLGTGHDLLREITQMQIDAARHANPLTQLPGN 422 Query: 435 VPINEHIEFLLQSGVEFCICYCDLDNFKPFNDVYGYRKGDDMIQLTGRTLSQFCDTQLDF 494 VPIN+HIE+LLQSGVEFCICYCDLD FKPFNDVYGYRKGDDMIQ TGR L Q CD +LDF Sbjct: 423 VPINDHIEYLLQSGVEFCICYCDLDQFKPFNDVYGYRKGDDMIQATGRVLQQACDPELDF 482 Query: 495 IGHIGGDDFLIMFQSEDWEHRCKAMLEAFAVASQSFYYVEDKERGGYYSEDRQGNRVLHP 554 IGHIGGDDFLI+FQSEDWE RC++ML+ F ++ FYY ED ERGGYY+EDRQGNRV H Sbjct: 483 IGHIGGDDFLILFQSEDWEKRCRSMLDNFDAETRGFYYDEDVERGGYYTEDRQGNRVFHK 542 Query: 555 LVSLSLGAVKIEPSQYSSPHQIASAATKAKKQAKKTPGNSLFIERRVPATFSWAM 609 LVS+SLGAVKIEPSQYSSPHQIASAAT+AKKQAKK PGNSLFIERR+PATFSWA+ Sbjct: 543 LVSVSLGAVKIEPSQYSSPHQIASAATRAKKQAKKIPGNSLFIERRIPATFSWAL 597 >gi|300313035|ref|YP_003777127.1| serine phosphatase, regulator of sigma subunit [Herbaspirillum seropedicae SmR1] Length = 422 Score = 101 bits (251), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 48/121 (39%), Positives = 77/121 (63%) Query: 303 DVSNDKVYNLFVNDPQLQALPVVSNGIPVGLINRYSMVERFARLYGRELYGKKSCTFFMD 362 D +N V +F +L +LPVV P+GLI+R+ + + ++ Y RELY +KSC FMD Sbjct: 33 DQNNAAVLEIFDQHKELVSLPVVEQQRPIGLISRHIFMSQMSKPYHRELYERKSCIAFMD 92 Query: 363 PNPLLTEQDTSLQDLSHILVQAESHHLSNGFIIVDQGQYVGMGTGHDLLREITQMQIEAA 422 +PL+ + DT ++ L + V + L++GF+IV+QGQ G+G+G L+RE+ +Q+E Sbjct: 93 KDPLIVDADTPIETLGALAVASGDKTLADGFMIVEQGQLNGLGSGLALMRELVDLQVEKN 152 Query: 423 R 423 R Sbjct: 153 R 153 >gi|300309883|ref|YP_003773975.1| GGDEF domain/EAL domain signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 97.8 bits (242), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 73/248 (29%), Positives = 111/248 (44%), Gaps = 20/248 (8%) Query: 28 QRSLTALFQPIIRMRSGEIVGYEGLIRGPSNSP-LHSPLNLFKVAR--AXPNKLSVQVEH 84 +R L+ FQPI+R+ G+++ +E L R S SP+ A N+LSV + Sbjct: 407 ERELSVFFQPIVRLDDGQVIAFEALARWHSTVKGAVSPVEFIPAAERMGIINQLSVILLR 466 Query: 85 LCRHIVMAQFAQSGLPGNLF---LNVSPGALVQPEAIQGETLRHLEKLGINPQRIIIELT 141 + AQ L NL +N S G E ++ + + G P R+ +E+T Sbjct: 467 KALAAAASWPAQIRLSFNLSANDINSSEG--------MAEIIKLIRESGFCPSRLDLEIT 518 Query: 142 ENEPTYDYTLLRNAAMHYRNMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFIQGINN 201 E D R R +G I +DD G G++SL L +K+D F+ I Sbjct: 519 ETALLQDLEKTRQLIHSLRQLGCGITLDDFGTGYASLSHLHALPLTRIKIDKSFVADIGQ 578 Query: 202 DPMKLQFVRSIQEIAEKSGSIVIAEGIETQAELLTIRDLGIACGQGYHIARPHANPKTVV 261 D + L+ V+S+ + + EG+ET L +R+LG GQGY ARP A Sbjct: 579 DAVSLKIVKSLLALTLDMDLACVVEGLETPQHLAVLRELGAMYGQGYLYARPMA------ 632 Query: 262 GDDISKVL 269 DD++ L Sbjct: 633 ADDVAPWL 640 >gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709 [Herbaspirillum seropedicae SmR1] Length = 598 Score = 93.2 bits (230), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 69/223 (30%), Positives = 107/223 (47%), Gaps = 7/223 (3%) Query: 35 FQPIIRMRSGEIVGYEGLIRGPSNSPLHS--PLNLFKVARAXPNKLSVQVEHLCRHIV-- 90 FQP +R+R G VG E LIR + P+H P ++F ++ + R +V Sbjct: 365 FQPKVRLRDGACVGVEALIRW--HHPIHGNIPPDVFIPLAEKTGQIRQVTAWVLRQVVAQ 422 Query: 91 MAQFAQSGLPGNLFLNVSPGALVQPEAIQGETLRHLEKLGINPQRIIIELTENEPTYDYT 150 +A++ +SG + N S E ++ + L+ + P I IE TE+ Sbjct: 423 VARWLKSGRALPVAFNASARDFDSDE-LRELLAQQLKLHKVPPALIEIEFTEHAMANHPQ 481 Query: 151 LLRNAAMHYRNMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFIQGINNDPMKLQFVR 210 +R R +G ++AIDD G GFS+L + + +K+D FI + +D V Sbjct: 482 QVRQRVQALRELGVKVAIDDFGAGFSNLSYLKNIPADTMKIDRSFIHRLLDDEADQLIVP 541 Query: 211 SIQEIAEKSGSIVIAEGIETQAELLTIRDLGIACGQGYHIARP 253 SI +A G V+AEG+E++ +R LG GQGY IARP Sbjct: 542 SILHLAHNLGFSVVAEGLESERTATLLRHLGCDYGQGYAIARP 584 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 85.5 bits (210), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 66/231 (28%), Positives = 102/231 (44%), Gaps = 23/231 (9%) Query: 35 FQPIIRMRSGEIVGYEGLIR---------GPSNSPLHSPLNLFKVARAXPNKLSVQVEHL 85 QP + + SG++VG E L+R GP N + F R + QV L Sbjct: 551 LQPKLELCSGQVVGAEALVRWIHPEKGFIGPDNFIPFAETTGF--IRMLTLWMMEQVARL 608 Query: 86 CRHIVMAQFAQSGLPGNLFLNVSPGALVQ---PEAIQGETLRHLEKLGINPQRIIIELTE 142 Q Q G+ +N+S L+ P A RH + P + +E+TE Sbjct: 609 SE-----QLQQQGVQLKFSVNLSTRDLLDQELPTAFAAILQRH----RLQPAALCLEITE 659 Query: 143 NEPTYDYTLLRNAAMHYRNMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFIQGINND 202 + D +N NMGF ++IDD G G+SSL L + +K+D F+ + D Sbjct: 660 SAIMDDPARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQD 719 Query: 203 PMKLQFVRSIQEIAEKSGSIVIAEGIETQAELLTIRDLGIACGQGYHIARP 253 + VRS ++ G V+AEG+E++ +R +G GQGY + +P Sbjct: 720 QDDAKIVRSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQGQGYFMGKP 770 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,900,974 Number of extensions: 79380 Number of successful extensions: 264 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 21 Length of query: 609 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 518 Effective length of database: 1,188,985 Effective search space: 615894230 Effective search space used: 615894230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)