BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0138.p (197 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311234|ref|YP_003775326.1| hypothetical protein Hsero_1909... 177 7e-46 >gi|300311234|ref|YP_003775326.1| hypothetical protein Hsero_1909 [Herbaspirillum seropedicae SmR1] Length = 257 Score = 177 bits (448), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 92/197 (46%), Positives = 129/197 (65%), Gaps = 8/197 (4%) Query: 5 PSQPPLIAYLIGFALSACCAIWAGSQFALTGSSIGYGVLTGLAFGAMFAFGQRVKNRLFP 64 P P++ L+G + +WAG + +S+ YG++ GL+FGA FAFGQ++KNR P Sbjct: 60 PRDVPVLFSLLGLVAAFALGLWAGIHYMSNDASLFYGLIAGLSFGAAFAFGQQIKNRFVP 119 Query: 65 TPESTK--WKINAGPSAADLRPAKRAAARAKPVTVAFDDEFIKTARAGAELESIAWQDVE 122 P++ K WK+ A P AA+L PA+ AA +A+ ++V FDDE I+T GA+ E IAW +V+ Sbjct: 120 -PKARKSDWKVQA-PGAAELSPARLAAQQARKISVEFDDEEIRTMVRGAKREGIAWAEVK 177 Query: 123 RIVITIGDDFLPMPYWMLA--TAKG--GIRLPNDTPGLESLMEEFKVKLPGYDNDATYAA 178 + I I + LP P W+L+ +A+G GI +PND GLE LM+ K +LPGYDND Y+ Sbjct: 178 DVTIRISEGHLPQPEWILSGQSAEGIKGILVPNDADGLEELMQAMKQRLPGYDNDQNYST 237 Query: 179 VIAAMGAMEGVFEVWKK 195 VIAAM AMEG F VW + Sbjct: 238 VIAAMSAMEGSFHVWSR 254 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 618,675 Number of extensions: 23139 Number of successful extensions: 84 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 82 Number of HSP's successfully gapped: 1 Length of query: 197 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 117 Effective length of database: 1,241,070 Effective search space: 145205190 Effective search space used: 145205190 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)