BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0133.p (317 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311227|ref|YP_003775319.1| thioredoxin reductase oxidoredu... 612 e-176 gi|300313594|ref|YP_003777686.1| alkyl hydroperoxide reductase s... 129 3e-31 gi|300310953|ref|YP_003775045.1| thioredoxin reductase oxidoredu... 62 9e-11 >gi|300311227|ref|YP_003775319.1| thioredoxin reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 612 bits (1578), Expect = e-176, Method: Compositional matrix adjust. Identities = 294/316 (93%), Positives = 306/316 (96%) Query: 2 TTPKHAKVLILGSGPAGYSAAVYAARANLNPVLITGVEQGGQLMTTTDVENWPGDPMGVQ 61 TTPKHAKVLILGSGPAGYSAAVYAARANLNPVLITGVEQGGQLMTTTDVENWPGDP+GVQ Sbjct: 3 TTPKHAKVLILGSGPAGYSAAVYAARANLNPVLITGVEQGGQLMTTTDVENWPGDPLGVQ 62 Query: 62 GPELMQRLLQHAERFNTEIIFDHIHTTRLSERPFRLIGDSGEYTCDALIIATGASAQYLG 121 GPELMQRLLQHAERF TEIIFDHIHTT+LSERPFRLIGDSGEYT DALIIATGASAQYLG Sbjct: 63 GPELMQRLLQHAERFKTEIIFDHIHTTKLSERPFRLIGDSGEYTADALIIATGASAQYLG 122 Query: 122 LPSEEAFMGKGVSACATCDGFFYRGKEVAVVGGGNTAVEEALYLSNIASKVTIIHRRDKF 181 LPSE+AFMG+GVSACATCDGFFYRGKEVAVVGGGNTAVEEALYLSNIASKVTIIHRRDKF Sbjct: 123 LPSEQAFMGRGVSACATCDGFFYRGKEVAVVGGGNTAVEEALYLSNIASKVTIIHRRDKF 182 Query: 182 RAEPILIDRLLHKVTEGKIDIKWHHTLDEVVGDESGVTGIKIKSTQDGSVTPITLHGLFI 241 RAEPILIDRLLHKV+EGKI I+WHHTLDEVVGD+SGVTGIKIKSTQD ++T I +HGLFI Sbjct: 183 RAEPILIDRLLHKVSEGKIAIQWHHTLDEVVGDDSGVTGIKIKSTQDDAITEIPVHGLFI 242 Query: 242 AIGHKPNTGIFEGQLDMHNGYIKTRTGLEGFATATSIDGVFAAGDVQDHVYRQAITSAGT 301 AIGHKPNT IFEGQL M NGY+KTRTG EGFATATSIDGVFAAGDVQDHVYRQAITSAGT Sbjct: 243 AIGHKPNTSIFEGQLSMQNGYLKTRTGTEGFATATSIDGVFAAGDVQDHVYRQAITSAGT 302 Query: 302 GCMAALDSQRYLEGLE 317 GCMAALD+QRYLEGLE Sbjct: 303 GCMAALDAQRYLEGLE 318 >gi|300313594|ref|YP_003777686.1| alkyl hydroperoxide reductase subunit F [Herbaspirillum seropedicae SmR1] Length = 533 Score = 129 bits (324), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 92/283 (32%), Positives = 146/283 (51%), Gaps = 26/283 (9%) Query: 41 GGQLMTTTDVENWPGDPMGVQ---GPELMQRLLQHAERFNTEIIFDHIHTTRLSERPFRL 97 GGQ++ T +EN+ + VQ GP+ L QH + ++ +I+ ++ +L Sbjct: 248 GGQVLDTLAIENF----ISVQETEGPQFATALEQHVKAYDVDIM--NVQRAEALVPGDKL 301 Query: 98 I----GDSGEYTCDALIIATGASAQYLGLPSEEAFMGKGVSACATCDGFFYRGKEVAVVG 153 I + G +I++TGA + + +P E+ + KGV+ C CDG ++GK V+V+G Sbjct: 302 IEVKLANGGVLKGKTVILSTGARWRNVNVPGEQEYKNKGVAYCPHCDGPLFKGKRVSVIG 361 Query: 154 GGNTAVEEALYLSNIASKVTIIHRRDKFRAEPILIDRL--LHKVTEGKIDIKWHHTLDEV 211 GGN+ VE A+ L+ I + VT+I + +A+ +L+ +L L VT I E+ Sbjct: 362 GGNSGVEAAIDLAGIVAHVTLIEFAAELKADAVLVRKLKSLPNVT-----IVTSAQTTEI 416 Query: 212 VGDESGVTGIKIKSTQDGSVTPITLHGLFIAIGHKPNTGIFEGQLDM-HNGYIKTRTGLE 270 GD V GI K + G + + L G+F+ IG PNT +G L + +G I+ Sbjct: 417 TGDGQRVNGISYKERETGEIKHVPLEGVFVQIGLVPNTEWLKGTLALSKHGEIEIDA--- 473 Query: 271 GFATATSIDGVFAAGDVQDHVYRQAITSAGTGCMAALDSQRYL 313 TSI GVFAAGDV Y+Q + + G G AAL + +L Sbjct: 474 --KGQTSIPGVFAAGDVTTVPYKQIVIAVGAGATAALSAFDHL 514 >gi|300310953|ref|YP_003775045.1| thioredoxin reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 61.6 bits (148), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 75/315 (23%), Positives = 136/315 (43%), Gaps = 38/315 (12%) Query: 7 AKVLILGSGPAG----YSAAVYAARANLNPVLITGVEQGGQLMTTTDVENWPGDPMGVQG 62 A +I+G+GP G + + +A++ L Q +L + + P P+ G Sbjct: 16 ADAVIIGAGPVGLFQVFELGLLEIKAHVIDSLPVVGGQCVELYPDKPIYDIPAVPV-CTG 74 Query: 63 PELMQRLLQHAERFNTEIIFDHIHTTRLSERP---FRLIGDSG-EYTCDALIIATGASA- 117 EL LL+ E F+ T +++R F + +G ++ + IA G + Sbjct: 75 QELTDNLLKQIEPFSPTFHLGQ-EVTLVNKREDGRFDVETSTGTKFITKTIFIAAGVGSF 133 Query: 118 --QYLGLPSEEAFMGKGVSACATCDGFFYRGKEVAVVGGGNTAVEEALYLSNIASKVTII 175 + L + E F G V D + GK + + GGG++A++ AL A V ++ Sbjct: 134 QPRTLKVEGIEQFEGSQVFYRVK-DPALFEGKNIVICGGGDSALDWALNFVGKAESVILL 192 Query: 176 HRRDKFRAEPILIDRLLHKVTEGKIDIKWHHTLDEVVGDESGVTGIKIKSTQ-------- 227 HRR++FRA P + ++ ++ + + ++G VTG +IK + Sbjct: 193 HRREEFRAAPASVAKMK--------ELCEQYEMQLLIGQ---VTGTEIKDGKLAEVKVTG 241 Query: 228 -DGSVTPITLHGLFIAIGHKPNTG-IFEGQLDMHNGYIKTRTGLEGFATATSIDGVFAAG 285 DG + L L + G P G I E L++ +K E F T++ G+FA G Sbjct: 242 ADGVTRRLPLDMLLVFFGLSPKLGPIAEWGLEIERKQLKV-MDTEKF--ETNVPGIFAVG 298 Query: 286 DVQDHVYRQAITSAG 300 D+ + ++ + +G Sbjct: 299 DINTYPGKKKLILSG 313 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 983,515 Number of extensions: 42256 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 3 Length of query: 317 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 232 Effective length of database: 1,217,395 Effective search space: 282435640 Effective search space used: 282435640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)