BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0129.p (337 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 276 2e-75 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 123 2e-29 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 93 3e-20 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 89 4e-19 gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 89 7e-19 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 276 bits (706), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 146/332 (43%), Positives = 204/332 (61%), Gaps = 8/332 (2%) Query: 4 RILGRNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYGMGHNEM 63 R LG NG SA+GLGCMGMSD Y D+ +ATI AL+ G+ LLDT D YG NE Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEE 77 Query: 64 LIREALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWV-GMDNRPAAVKNFLAYTLRRLGT 122 L+ A++G RDK ++ KFG RD G+D PA ++ + +L+RLG Sbjct: 78 LLGRAIKG-------RRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGV 130 Query: 123 DHIDIYRPARLDPSVPIEDTVGAIAEEIKAGRVRHIGLSEVGADTLRRAHAVHPITDLQI 182 + ID+Y R+DP+ PIE T+G +A+ ++AG++R+IGLSE A TL RAH VHP+T LQ Sbjct: 131 ETIDLYYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSEASAATLERAHRVHPVTALQS 190 Query: 183 EYSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHWDPSRQLAANDFRSHSPRFSGD 242 EYSL +R E ++L CR LGIG AY L RG ++G LA +DFR +PRF G+ Sbjct: 191 EYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFRRSNPRFQGE 250 Query: 243 NLAHNLSLIDVLRSVAADKQATPAQVAIAWVLAQGSDIVPLVGARRPERLAESLGALKLT 302 N A NL L + ++ +A TP+Q+A+AWV+AQ IVP+ G +R L ++ G++ + Sbjct: 251 NFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVK 310 Query: 303 LTAQDLARIEEAIPADAAAGDRYAAHAMAHLD 334 L +DL ++ P AAAG+RY +M L+ Sbjct: 311 LAPEDLQALDAIFPRGAAAGERYTDASMKMLN 342 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 104/328 (31%), Positives = 162/328 (49%), Gaps = 29/328 (8%) Query: 1 MEHRILGRNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYG--M 58 M +R GR+GL A+ LG + G + + G A + A D GVT D + YG Sbjct: 14 MPYRRTGRSGLLLPAISLGLW--QNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPPA 71 Query: 59 GHNEMLIREALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWVGMDNRPAAVKNFL----A 114 G E R RD++ +S K G W G P+ + L Sbjct: 72 GSAE---ENFGRWMAKDFLPYRDELVISSKAGWQM-----WPGPYGGPSGARKHLIASCE 123 Query: 115 YTLRRLGTDHIDIYRPARLDPSVPIEDTVGAIAEEIKAGRVRHIGLSEVGADTLRRAHAV 174 +L+R+G D++DI+ R+D P+E+T+GA+A + G+ ++G+S + R+A A+ Sbjct: 124 QSLKRMGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAI 183 Query: 175 H-----PITDLQIEYSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHWDPSRQLAA 229 P+ Q YS+++R IE +LP ELG+G + L++GL++ + A Sbjct: 184 LKSEGVPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVPEGA 243 Query: 230 NDFRSHS-PR--FSGDNLAHNLSLIDVLRSVAADKQATPAQVAIAWVLAQGSDIVPLVGA 286 R+ S P+ S DNLA I L +A+ + T AQ+AIAWVL L+GA Sbjct: 244 RVTRAPSLPQRMLSEDNLAR----IRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGA 299 Query: 287 RRPERLAESLGAL-KLTLTAQDLARIEE 313 R E+L +SL A+ KL T ++LA I++ Sbjct: 300 RTVEQLDDSLDAVNKLDFTQEELASIDK 327 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 92.8 bits (229), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 87/345 (25%), Positives = 153/345 (44%), Gaps = 44/345 (12%) Query: 1 MEHRILGRNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYGMGH 60 M +R LG++ L SAL LG M D D Q + +A + GV +DT D Y G Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGD---QTDLQESGRIVASAREHGVNFIDTADVYTKGK 57 Query: 61 NEMLIREALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWVGMDNRPAAVKNFLAYTLR-- 118 +E ++ + L G R + L+ K G M +P ++ +R Sbjct: 58 SEEIVGKLLAG-------QRHEWVLASKLGN---------AMSGKPNESNYSRSWIVRET 101 Query: 119 -----RLGTDHIDIYRPARLDPSVPIEDTVGAIAEEIKAGRVRHIGLSEVG----ADTLR 169 RL TD++DI R +E+ V A+ + I+ G++R G+S A+ +R Sbjct: 102 ENILTRLDTDYLDILYMHRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVR 161 Query: 170 ---RAHAVHPITDLQIEYSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHWDPSRQ 226 + + P+ Q Y+L++R E +ILP C G+G+ Y ++RG+++G + P ++ Sbjct: 162 LCEKNNVPLPLV-CQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQK 220 Query: 227 LAAN------DFRSHSPRFSGDNLAHNLSLIDVLRSVAADKQATPAQVAIAWVLAQGSDI 280 A + D R F ++L + L+ + Q A AWVLA + Sbjct: 221 PAQDTRAGRGDKRMMETEFREESLL----IAQRLQQHCEARGVKLGQFATAWVLANSAIS 276 Query: 281 VPLVGARRPERLAESLGALKLTLTAQDLARIEEAIPADAAAGDRY 325 + G R ++ + A +L ++A++ A ++ + A+ Y Sbjct: 277 SVIAGPRTLAQMEDYYDAAELKISAEEEALVDSLVTPGHASTHGY 321 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 82/300 (27%), Positives = 137/300 (45%), Gaps = 25/300 (8%) Query: 1 MEHRILGRNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYGMGH 60 M+ R GR G S +G G + +G Q A +HAALDAGV+ +DT D YG G Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 Query: 61 NEMLIREALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWVGMDNRPAAVKNFLAYTLRRL 120 +E LI + L+ +GG ++ ++ K G R + N A + F+ +L+ L Sbjct: 61 SEQLIAQVLK---ERGG---ERPFVATKAG--RRLSPHVASGYNDRAQLSAFIDRSLKNL 112 Query: 121 GTDHIDIYRPARLDPSVPIEDTV-GAIAEEIKAGRVRHIGLSEVGADTLRRAHAVHPITD 179 G D +D+ + V + V G + + AG++RH G+S + +A + Sbjct: 113 GVDCLDLVQLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKS 172 Query: 180 LQIEYSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHWDPSRQLAANDFRSHS--- 236 +QI +++ + L + I + A L+ GL++G AA+D R + Sbjct: 173 VQIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHG 232 Query: 237 ------PRFSGDNLAHNLSLIDVLRSVAADKQATP-AQVAIAWVLAQG--SDIVPLVGAR 287 FSG L+ ++ +R++ Q P AQ A+ W+L + S ++P GAR Sbjct: 233 EAFDVGETFSGVPYEVALAAVEKIRALV--PQGVPMAQFALRWILMESGISTVIP--GAR 288 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 88.6 bits (218), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 85/312 (27%), Positives = 136/312 (43%), Gaps = 57/312 (18%) Query: 8 RNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYGMGHNEMLIRE 67 RNG S LG G M + PA A + + A LD G++L+DT + Y G E ++ + Sbjct: 10 RNGQSVPILGQGTWNMGE--SPAQANAEVRALQAGLDLGMSLIDTAEMYAEGGAERIVAK 67 Query: 68 ALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWVGMDNRPAAVKNFLAYTLRRLGTDHIDI 127 A+ G RD+V L K + + AA + +L+RLGTD ID+ Sbjct: 68 AIAG-------RRDQVYLVSKVLPHNASKRGTI------AACEA----SLKRLGTDRIDL 110 Query: 128 YRPARLDPSVPIEDTVGAIAEEIKAGRVRHIGLSEVGAD----TLRRAHAVHPITDLQIE 183 Y + P+ T+ + + G++ G+S D L H H + + Q+ Sbjct: 111 YL-LHWRGAHPLAATIEGMETLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCN-QVL 168 Query: 184 YSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHWDPSRQLAANDFRSHSPRFSGDN 243 Y+L RGIE D+LP ++ I + AY + +G I H Sbjct: 169 YNLSRRGIEYDLLPQAQQAHIAVMAYSPIEQGRILKH----------------------- 205 Query: 244 LAHNLSLIDVLRSVAADKQATPAQVAIAWVLAQGSDIVPLVGARRPERLAESLGALKLTL 303 L ++A + TPAQ+A+AWVL Q +V + A + + +L ++L Sbjct: 206 --------PALTALAQAHRVTPAQIALAWVLRQ-PGVVAIPKAADVAHVQANRASLDISL 256 Query: 304 TAQDLARIEEAI 315 +A DL ++EA Sbjct: 257 SAADLKILDEAF 268 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,088,889 Number of extensions: 51054 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 6 Length of query: 337 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 251 Effective length of database: 1,212,660 Effective search space: 304377660 Effective search space used: 304377660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)