BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0124.p (531 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) syntheta... 345 4e-96 gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthet... 145 8e-36 >gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) synthetase [Herbaspirillum seropedicae SmR1] Length = 565 Score = 345 bits (886), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 218/565 (38%), Positives = 309/565 (54%), Gaps = 39/565 (6%) Query: 3 VKVAIAQINSTVGDISGNRNKIVEFARRAAAQGADIVLTPELSLVGYPPEDLLLRQAFYA 62 +++AIAQ N T+GD GN ++ +RAA GAD+V+ PELS+ GY P DLL F Sbjct: 2 LRIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFLD 61 Query: 63 KTEEALQALTAELAGLKDVHVVVGHPALKDGAR---FNAASVLLNGKITGSYRKLELPND 119 + E LQ + L + V+G P G FNA + G+I Y K LP Sbjct: 62 RAEATLQDVIDATRRLPGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPVY 121 Query: 120 SVFDEKRYFT--PDGTPLVFDVKGTKFGINICEDTW------FPRAP-ARAKEAGAQVLL 170 VFD+ R+F P+ T V +KG + G ICED W + P + +A AQ+++ Sbjct: 122 GVFDDGRHFEAGPE-TACVLPLKGMRVGFLICEDGWNDDLRSYRVNPFEKLAQADAQLVV 180 Query: 171 ILNGSPYHMNKQLLRLDVMRDNVTKQGMPLVFANLVGGQDELIFDGNSFAMDIAGELVAS 230 +N SP + K+ R + + ++ M L++ N VGGQD+L++DG SFAM L Sbjct: 181 SINASPSDVGKRSQRHALFQAACLRRQMALLYVNQVGGQDQLVYDGGSFAMSPLQGLAFE 240 Query: 231 LAHAQEDLQVIEFDGVQ------IRNPQMAPALSIEAQVYQ-ALVLGVRDYVGKNGFPSV 283 A ED QV+ F+G + + PQ + ++ ++LG+RDY + GF V Sbjct: 241 AARLAEDFQVLAFEGGRFLRSDGVGAPQCIDQTMATPEFHRRQILLGLRDYARRCGFGKV 300 Query: 284 LIGLSGGVDSA------XDALGADKVRAIMMPSPYTADISWIDSRDMVERIKVRYDEISI 337 ++G SGG+DSA +ALGA V A+ MPS +++ S DS + + + V I Sbjct: 301 VVGSSGGIDSAVTIALAAEALGAQNVVAVTMPSVFSSAGSVSDSETLCQALGVTLFRHPI 360 Query: 338 NDCFSAFRSTLSEEFKGLKEDTTEENIQARIRGTLLMALSNKYGSIVLTTGNKSEMAVGY 397 D F + F EN+QAR+RG +LM SN Y +++LTTGNKSE++VGY Sbjct: 361 ADLVREFEDGFATAFGAPLRGLPLENLQARVRGAILMEYSNGYDALLLTTGNKSEISVGY 420 Query: 398 CTLYGDMAGGFAVIKDIAKTLVYRLCAYRNSIS--EVIPERILTRAPSAELRPDQKDQDS 455 CTLYGD GG +I D+ KT VY L + N+ + E+IP I+ +APSAEL PDQ+D DS Sbjct: 421 CTLYGDTNGGLGLIGDLYKTEVYALARHLNAQAGREIIPAAIIDKAPSAELAPDQRDTDS 480 Query: 456 LPPYEVLDAIMQMYME-ENLSIGEIERA--------GYQKGD--IERITRLIKINEYKRR 504 LPPYEVLD I++ ++E L E + A G G +ERI R++ NEYKRR Sbjct: 481 LPPYEVLDEILKWHIEGSRLPADEAQAAARTVEQLSGTDTGAALVERICRMVARNEYKRR 540 Query: 505 QAPVGIRVTHRAFGRDWRYPITSRF 529 Q+P IR+ RAFG + PI R+ Sbjct: 541 QSPPVIRLRSRAFGSGRQIPIAVRY 565 >gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthetase [Herbaspirillum seropedicae SmR1] Length = 685 Score = 145 bits (366), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 151/562 (26%), Positives = 239/562 (42%), Gaps = 104/562 (18%) Query: 4 KVAIAQINSTVGDISGNRNKIVEFARRAAAQGADIVLTPELSLVGYPPEDLLLRQAFYAK 63 +VA+A + V D N + + A +AAA+GA +V PEL L Y EDL ++A Sbjct: 15 RVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAYSCEDLFQQRALLDA 74 Query: 64 TEEALQALTAELAGLKDVHVVVGHPALKDGARFNAASVLLNGKITGSYRKLELPNDSVFD 123 + +ALQ++ E + ++VG P D +N A VL +G+I G K LPN S F Sbjct: 75 SLQALQSVL-EASMRIPAALIVGLPLKVDHQLYNCAVVLHHGRILGVVPKSYLPNYSEFY 133 Query: 124 EKRYFTPD-----------------GTPLVFDVKGT---KFGINICEDTWFPRAPAR-AK 162 E R F+ G+ L+F+++ +F I ICED W P P+ A Sbjct: 134 EARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICEDVWVPIPPSSFAA 193 Query: 163 EAGAQVLLILNGSPYHMNKQLLRLDVMRDNVTKQGMPLVFANLVGGQD--ELIFDGNSFA 220 AGA VL+ L+ S + K R ++ + ++++ G+ +L +DG + Sbjct: 194 LAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGESTTDLAWDGQALI 253 Query: 221 MDIAGELVA---------------------------------SLAHAQEDLQVIEFDGVQ 247 + GEL+A S+ ++++ E G + Sbjct: 254 CE-NGELLAESERFAQGGHVIYADVDLERLSRERFHQTTFGQSVRRHADEVRRFEVVGFE 312 Query: 248 IRNPQMA-----------PALSIEA--------QVYQALVLGVRDYVGKNGFPSVLIGLS 288 ++ PQ P + A +VY V + + G V+IG+S Sbjct: 313 VQIPQQQALPLQRRVARFPYVPANAEQRELRCREVYSIQVQALVQRLSSAGLKKVVIGVS 372 Query: 289 GGVDS---------AXDALGADK--VRAIMMPSPYTADISWIDSRDMVERIKVRYDEISI 337 GG+DS A D LG + + A MP T+ + + + ++ ++ EI I Sbjct: 373 GGLDSTHALLVCAKAMDKLGLPRSNILAYTMPGFATSSRTLVQAHQLMAQVGCSAQEIDI 432 Query: 338 NDCFSAFRSTLSEEFKGLKE--DTTEENIQARIRGTLLMALSNKYGSIVLTTGNKSEMAV 395 L + + D T EN+QA R L L+N +G+IV+ TG+ SE+A+ Sbjct: 433 RPSCEQMLKDLGHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAIVIGTGDLSELAL 492 Query: 396 GYCTL-YGDMAGGFAVIKDIAKTLV---YRLCAYRNSISEVIPE---RILTRAPSAEL-- 446 G+CT GD + V + KTL+ R A S+ PE +L S EL Sbjct: 493 GWCTYGVGDHMSHYNVNASVPKTLITHMVRWVAESGSLELKQPEVLIHVLETEISPELVP 552 Query: 447 -----RPDQKDQDSLPPYEVLD 463 P Q+ QD + PYE+ D Sbjct: 553 GASDGEPGQRTQDFIGPYELQD 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,603,881 Number of extensions: 66842 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 3 Length of query: 531 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 441 Effective length of database: 1,193,720 Effective search space: 526430520 Effective search space used: 526430520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)