BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0114.p (275 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312232|ref|YP_003776324.1| signal transduction protein [He... 431 e-122 gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626... 236 1e-63 gi|300311595|ref|YP_003775687.1| two component response regulato... 79 3e-16 gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 77 1e-15 >gi|300312232|ref|YP_003776324.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 431 bits (1109), Expect = e-122, Method: Compositional matrix adjust. Identities = 204/275 (74%), Positives = 237/275 (86%), Gaps = 1/275 (0%) Query: 1 MNFEALFQQQAALPSIPKVVQEVIESFNNDNVSIEDIAKKLSADQVLSAKLLRLANSSYY 60 MN EALFQQQ ALPSIPKVVQEVIESFNN++VSIE+IAKKL+ADQVLSAK+LRLANSSYY Sbjct: 1 MNLEALFQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYY 60 Query: 61 HVSRSIGTVDDAVLMLGFMTVRTLVVSTGLTGSFKSMSGVDLKLFWRYSLHTAIVAKWLA 120 HVSR+IGTVDDAVLMLGFMTVRTLVVSTG+ GSFK + GVDLKLFWRYSL TA+VAKWLA Sbjct: 61 HVSRTIGTVDDAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKLFWRYSLDTALVAKWLA 120 Query: 121 KKQGLNSDLAFTVGLMHAIGQLVMHAAMPEQTRQIDKIASTIDARRLDVEHTSFGYDFSD 180 K+ LNSD AFTVGLMHAIGQLVMHA MPEQ Q+DK+A +DARRLDVE SFGYDF+D Sbjct: 121 KRGKLNSDFAFTVGLMHAIGQLVMHAGMPEQALQVDKVAGPLDARRLDVERKSFGYDFAD 180 Query: 181 VGAELAKRWRFPEEFSEAIAAFSSPLEADPFNKIAAVIHIAAWRARAEENNYSREEMEST 240 VGAELA+RWRFP+EF++ I AF PL+ + F+ AA++H+AAWRARA EN YS EE+ +T Sbjct: 181 VGAELARRWRFPDEFAKVIEAFPQPLQGE-FDPFAAIVHLAAWRARAHENQYSDEELAAT 239 Query: 241 FPDDVAAKLGVKQAVLFDDLPPLAELGAGMEELIS 275 FP +VA +LGV+ ++PPL ELG GM+ELI+ Sbjct: 240 FPAEVAERLGVRLETFTQEMPPLEELGEGMQELIA 274 >gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626 [Herbaspirillum seropedicae SmR1] Length = 290 Score = 236 bits (602), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 117/289 (40%), Positives = 181/289 (62%), Gaps = 15/289 (5%) Query: 1 MNFEALFQQQAALPSIPKVVQEVIESFNNDNVSIEDIAKKLSADQVLSAKLLRLANSSYY 60 M ++L Q LP++PKVVQ+++ SFN +++S +IA++++ D LSAK+LRLANS+++ Sbjct: 1 MELKSLVDQPGKLPTVPKVVQQLMASFNAEDISAHEIAQQIATDPALSAKVLRLANSAFF 60 Query: 61 HVSRSIGTVDDAVLMLGFMTVRTLVVSTGLTGSFKSMSGVDLKLFWRYSLHTAIVAKWLA 120 H SR+IGTVDDA+ +LGF+ VR LV+ G+ +FK+ G+DL FWR++L+TA+ A+WLA Sbjct: 61 HASRTIGTVDDALRILGFIMVRNLVLGNGMVAAFKNTRGMDLHQFWRHTLYTAVAARWLA 120 Query: 121 KKQGL----NSDLAFTVGLMHAIGQLVMHAAMPEQTRQIDKIASTIDARRLDVEHTSFGY 176 G+ N D FTVG+MH IGQL +HA +D+ ++ +D+ R + E ++G+ Sbjct: 121 MHAGMVGEDNGDAVFTVGMMHGIGQLQLHAVAANAVAPLDRHSNVLDSDRAEREKEAWGF 180 Query: 177 DFSDVGAELAKRWRFPEEFSEAIAAFSSPLEADPFNKIAAVIHIAAWRARAEENNYSREE 236 ++DV AELA W FP+ A+ + PL A F++ AA +H+ W AR + E Sbjct: 181 HYADVSAELAAIWHFPDALVTALRSVPDPLGAGRFDRAAAWVHLGGWAARVAILSQDDEN 240 Query: 237 MESTFPDDVAAKLGVKQA-----VLFDD------LPPLAELGAGMEELI 274 ++T+P V KLGV A V D +P L EL AG++ ++ Sbjct: 241 AQATYPAAVGKKLGVDPAWMPLLVKHDPDKAERAMPSLGELAAGLDAML 289 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 79.3 bits (194), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 53/203 (26%), Positives = 100/203 (49%), Gaps = 5/203 (2%) Query: 7 FQQQAALPSIPKVVQEVIESFNNDNVSIEDIAKKLSADQVLSAKLLRLANSSYYHVSRSI 66 Q LP+ V ++++ +++++ ++ ++ D VL+ +L+++AN + R + Sbjct: 13 MQDSEVLPAPSGVRLKIMQMCQQEDIALPELVAQVQGDPVLAGRLIQIANIPNINKGRMV 72 Query: 67 GTVD-DAVLMLGFMTVRTLVVSTGLTGSFK--SMSGVDLKLFWRYSLHTAIVAKWLAKK- 122 +V+ + +LM+G VR + ++ LT S + + D FW S A + L Sbjct: 73 ASVNVELLLMVGLHAVRQMALAISLTESARRGDCAEFDYDAFWSRSTAMACAGQALGDLL 132 Query: 123 QGLNSDLAFTVGLMHAIGQLVMHAAMPEQTRQIDKIASTIDARRLDVEHTSFGYDFSDVG 182 Q FT+GL+ IGQL + +A P++ ++ +A DA+ E SFG+D + Sbjct: 133 QVAPPAEMFTIGLLAGIGQLGLASARPKRYGEL-LLAGAKDAQLRVQERESFGFDHLALA 191 Query: 183 AELAKRWRFPEEFSEAIAAFSSP 205 A + + W FP F EA+ P Sbjct: 192 AAMMQDWHFPRIFCEAVLHHRDP 214 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 57/205 (27%), Positives = 97/205 (47%), Gaps = 17/205 (8%) Query: 12 ALPSIPKVVQEVIESFNNDNVSIEDIAKKLSADQVLSAKLLRLANSSYYHVSRSIGTVD- 70 ALPS V ++ DN + ++ + AD LS +++R+ N R I +V Sbjct: 18 ALPSPGGVRLTIMRLCAQDNPDMAELIHLVQADPALSGRIIRIVNVLNAQRVRPIASVSM 77 Query: 71 DAVLMLGFMTVRTLVVSTGL--TGSFKSMSGVDLKLFWRYSLHTAIVAKWLAKKQGLNSD 128 D++L++G VR L ++ L T + S + L FW +S A A Q + S Sbjct: 78 DSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFAMACSA------QAVTSQ 131 Query: 129 L-------AFTVGLMHAIGQLVMHAAMPEQTRQIDKIASTIDARRLDVEHTSFGYDFSDV 181 L FT GL+ IG+L + +A P + A ARR +E + FG++ ++ Sbjct: 132 LRVAPLGEMFTTGLLANIGKLTLASAQPTGYSALLTRALDAPARR-AMEQSLFGFNHLNI 190 Query: 182 GAELAKRWRFPEEFSEAIAAFSSPL 206 A L + W+ P+ F +A+ + +P+ Sbjct: 191 AAALMQDWKIPDLFCDAVLHYETPV 215 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 800,623 Number of extensions: 30767 Number of successful extensions: 138 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 133 Number of HSP's successfully gapped: 4 Length of query: 275 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 191 Effective length of database: 1,222,130 Effective search space: 233426830 Effective search space used: 233426830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)