BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0111.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312237|ref|YP_003776329.1| amino acid ABC transporter perm... 473 e-135 gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC tr... 171 5e-44 gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC t... 144 1e-35 gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC t... 144 1e-35 gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC t... 127 9e-31 >gi|300312237|ref|YP_003776329.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 473 bits (1217), Expect = e-135, Method: Compositional matrix adjust. Identities = 260/294 (88%), Positives = 277/294 (94%), Gaps = 1/294 (0%) Query: 1 MLEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEKMALPLW 60 MLEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVE+MALPLW Sbjct: 1 MLEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMALPLW 60 Query: 61 LAMLAAMVATGLIGLIVDILVLKPLRKRNAPHLIPMIATIGXCTPPDSHDRHHRRGAENK 120 +A+L AMVATGL+GLI+D+LVLKPLRKRNAPHLIPMIATIG ++ GAENK Sbjct: 61 VALLLAMVATGLLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIM-ITNISQGLFGAENK 119 Query: 121 RFPIGTIPEDSVTIGNLHVTAVQVGIVVIAFVLMIVLLAVMRRTQLGRALRAIAESPKAA 180 RFP GTIPE+SV++GNLH+TAVQV I+VIAFVLM+VLL VMR+TQLGRALRAIAESPKAA Sbjct: 120 RFPQGTIPEESVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAESPKAA 179 Query: 181 YLLGINVEGLFYLTSFAAAALGGAAGVLVGLSFNAISPFMGQPMLHKGIAVIILGGMGDI 240 YLLGINVEGLF LTSFAAAALGGAAGVLVG+SFNAISPFMGQPMLHKGIAVIILGGMGDI Sbjct: 180 YLLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILGGMGDI 239 Query: 241 RGAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVRPSGMFGKVLERKA 294 RGAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLV+PSGMFGKVLERKA Sbjct: 240 RGAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGKVLERKA 293 >gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 308 Score = 171 bits (434), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 107/298 (35%), Positives = 172/298 (57%), Gaps = 14/298 (4%) Query: 2 LEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEKMALP--- 58 L QQL+N LSLG++YAL A+G+T+V+G++G+IN +HG I+M+ SY L+ + + + Sbjct: 8 LTQQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAIGVQSGY 67 Query: 59 -----LWLAMLAAMVATGLIGLIVDILVLKPLRKRNAPHLIPMIATIGXCTPPDSHDRHH 113 L A++ +++ TGL G V+ + +PLR P L+P+I+ IG ++ + Sbjct: 68 PLPLLLGGALIVSVLVTGLYGWTVERVAYRPLR--GGPRLVPLISAIGMSIFLQNYVQIG 125 Query: 114 RRGAENKRFPI---GTIPEDSVTIGNLHVTAVQVGIVVIAFVLMIVLLAVMRRTQLGRAL 170 + GA + P+ G + + + + ++ IV + VLM+ L + R+++GRA Sbjct: 126 Q-GARDMSVPVLISGALEFQMGSDFTVTIPYSRMVIVGVTLVLMVALTLFIARSRMGRAC 184 Query: 171 RAIAESPKAAYLLGINVEGLFYLTSFAAAALGGAAGVLVGLSFNAISPFMGQPMLHKGIA 230 RA AE A LLGI+ + T A L GVL+ L+ ++P++G K Sbjct: 185 RACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFIAGIKAFT 244 Query: 231 VIILGGMGDIRGAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVRPSGMFGK 288 +LGG+G I GAM+GG+ LG AE Y+ S+++D V+FGLL LILL RP+G+ GK Sbjct: 245 AAVLGGIGSIPGAMLGGVLLGLAETFAAGYLPSEYKDVVSFGLLVLILLFRPTGLLGK 302 >gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 144 bits (362), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 107/308 (34%), Positives = 176/308 (57%), Gaps = 21/308 (6%) Query: 4 QQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLL---VEKMA--LP 58 QQ+IN L LGS+YAL ALG+T+V+GVL +IN +HG I M+G+ L L V+++A LP Sbjct: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAPGLP 65 Query: 59 ----LWLAMLAAMVATGLIGLIVDILVLKPLRKRNAPHLIPMIATIGXCTPPDSHDRHHR 114 L +A++ A+ ++ L+++ + +PLR NAP L P+I IG + Sbjct: 66 GIVQLVIAIVGAIPVCIVVSLLIERIAYRPLR--NAPRLAPLITAIGVSILLQTL-AMMI 122 Query: 115 RGAENKRFPIGTIPEDSVTIGNLHVTAVQVGIVVIAFVLMIVLLAVMRRTQLGRALRAIA 174 G FP +P D V I ++ Q+ ++ +A + M+ L+ ++ +T++GRA+RA A Sbjct: 123 WGRSPLPFP-QVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRATA 181 Query: 175 ESPKAAYLLGINVEGLFYLTSFAAAALGGAAGVLVGLSFNAISPFMGQPMLHKGIAVIIL 234 E+P+ A L+G++ + +T A L AGV+ +++ MG K + +L Sbjct: 182 ENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAVL 241 Query: 235 GGMGDIRGAMIGGLFLGFAEVLTVAYI--------SSDFRDAVAFGLLFLILLVRPSGMF 286 GG+G+I GAM+GG+ LG E L YI S+++D AF +L ++L +RPSG+ Sbjct: 242 GGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGIM 301 Query: 287 GKVLERKA 294 G+ + +A Sbjct: 302 GERVADRA 309 >gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 144 bits (362), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 107/308 (34%), Positives = 176/308 (57%), Gaps = 21/308 (6%) Query: 4 QQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLL---VEKMA--LP 58 QQ+IN L LGS+YAL ALG+T+V+GVL +IN +HG I M+G+ L L V+++A LP Sbjct: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAPGLP 65 Query: 59 ----LWLAMLAAMVATGLIGLIVDILVLKPLRKRNAPHLIPMIATIGXCTPPDSHDRHHR 114 L +A++ A+ ++ L+++ + +PLR NAP L P+I IG + Sbjct: 66 GIVQLVIAIVGAIPVCIVVSLLIERIAYRPLR--NAPRLAPLITAIGVSILLQTL-AMMI 122 Query: 115 RGAENKRFPIGTIPEDSVTIGNLHVTAVQVGIVVIAFVLMIVLLAVMRRTQLGRALRAIA 174 G FP +P D V I ++ Q+ ++ +A + M+ L+ ++ +T++GRA+RA A Sbjct: 123 WGRSPLPFP-QVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRATA 181 Query: 175 ESPKAAYLLGINVEGLFYLTSFAAAALGGAAGVLVGLSFNAISPFMGQPMLHKGIAVIIL 234 E+P+ A L+G++ + +T A L AGV+ +++ MG K + +L Sbjct: 182 ENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAVL 241 Query: 235 GGMGDIRGAMIGGLFLGFAEVLTVAYI--------SSDFRDAVAFGLLFLILLVRPSGMF 286 GG+G+I GAM+GG+ LG E L YI S+++D AF +L ++L +RPSG+ Sbjct: 242 GGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGIM 301 Query: 287 GKVLERKA 294 G+ + +A Sbjct: 302 GERVADRA 309 >gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 127 bits (320), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 87/276 (31%), Positives = 150/276 (54%), Gaps = 5/276 (1%) Query: 13 GSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEKMALPLWLAMLAAMVATGL 72 GS YA+ +LG ++FG+L VIN +HG ++MLG++ A + + + L W+ ++ A + G Sbjct: 22 GSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLSYLGLNYWVMLIVAPILVGA 81 Query: 73 IGLIVDILVLKPLRKRNAPHLIPMIATIGXCTPPDSHDRHHRRGAENKRFPIGTIPEDSV 132 G++++ +L+ L K + HL ++ T G + R G + + + + + + Sbjct: 82 FGIVIEKTMLRWLYKLD--HLYGLLLTFGITLMIEGIFR-SIYGVSGQPYSVPDLLQGAT 138 Query: 133 TIGNLHVTAVQVGIVVIAFVLMIVLLAVMRRTQLGRALRAIAESPKAAYLLGINVEGLFY 192 +G + + + +VV + ++ V+ +T+LG LRA E+PK GINV + Sbjct: 139 NLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGTENPKLVEAFGINVPLMVT 198 Query: 193 LTSFAAAALGGAAGVLVGLSFNAISPFMGQPMLHKGIAVIILGGMGDIRGAMIGGLFLGF 252 LT AL AGVL +SP MG ++ AV+++GGMG I G++I GL LG Sbjct: 199 LTYGFGVALAAFAGVLAAPVIQ-VSPLMGSNLIITVFAVVVIGGMGSILGSIITGLVLGL 257 Query: 253 AEVLTVAYISSDFRDAVAFGLLFLILLVRPSGMFGK 288 E LT + + V F ++ ++L+VRP+G+FGK Sbjct: 258 IEGLTRVF-YPELSATVVFIIMAIVLMVRPAGLFGK 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.145 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 759,237 Number of extensions: 26836 Number of successful extensions: 244 Number of sequences better than 1.0e-08: 28 Number of HSP's gapped: 209 Number of HSP's successfully gapped: 29 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)