BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0110.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312238|ref|YP_003776330.1| amino acid ABC transporter perm... 483 e-137 gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC t... 154 8e-39 gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC t... 138 5e-34 gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 138 7e-34 gi|300310522|ref|YP_003774614.1| branched-chain amino acid ABC t... 132 5e-32 >gi|300312238|ref|YP_003776330.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 483 bits (1242), Expect = e-137, Method: Compositional matrix adjust. Identities = 264/296 (89%), Positives = 279/296 (94%), Gaps = 8/296 (2%) Query: 4 MEWWDGFWATYNTVIFSVGVNAMLALSIYVTLSCGLLSLANAAFMGIGAYVASLITMQAG 63 MEWWDGFWATYNTVIFS+GVNAMLALSIYVTLSCGLLSLANAAFMGIGAY ASL+TMQ Sbjct: 1 MEWWDGFWATYNTVIFSIGVNAMLALSIYVTLSCGLLSLANAAFMGIGAYTASLLTMQFE 60 Query: 64 LPFPVALAAGGVLPALVALLIGIPTLRLSGVYLAMATLGFGEVVRVVVLNMEITGGPMGL 123 +PFP+ALAAGGVLPALVAL+IGIPTLRLSGVYLAMATLGFGEVVRV+VLN++ITGGPMGL Sbjct: 61 MPFPIALAAGGVLPALVALVIGIPTLRLSGVYLAMATLGFGEVVRVIVLNLDITGGPMGL 120 Query: 124 NGVPPVTEWWHIVLLLGVTVYFLARVRRSKIGRAFEAIKEDEVAARLMGVNVAGYKLLAF 183 NGVPP+TEWWHIVLLLGVT+Y LAR+RRSKIGRAFEAIKEDE+AARLMGVNVAGYKLLAF Sbjct: 121 NGVPPITEWWHIVLLLGVTIYVLARLRRSKIGRAFEAIKEDEIAARLMGVNVAGYKLLAF 180 Query: 184 VIGAAIAGVAGALNAHYTFTIGPNNYGFENAVDILTMAVFGGTSNLIGPMIGSTILSLLP 243 VIGAAIAGVAG LNAH+TFTIGPNNYGFENAVDILTMAVFGGTSNLIGPMIGSTILSLLP Sbjct: 181 VIGAAIAGVAGGLNAHFTFTIGPNNYGFENAVDILTMAVFGGTSNLIGPMIGSTILSLLP 240 Query: 244 EVLRHFKDFRLAANGLILILVVLYLPKGIWDPRRIRAFMQRRNSGNSNNSASKKVN 299 EVLRHFKDFRLA NGLILILVVLYLPKGIWDPRRIRAF+ R +A KKV Sbjct: 241 EVLRHFKDFRLAINGLILILVVLYLPKGIWDPRRIRAFLGR--------NAEKKVK 288 >gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 408 Score = 154 bits (389), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 100/279 (35%), Positives = 156/279 (55%), Gaps = 43/279 (15%) Query: 38 GLLSLANAAFMGIGAYVASLITMQAGLPF----PVALAAGGVLPALVALLIGIPTLRLSG 93 GLL L F +GAY +L GL F P+A AA AL L+G P LRL G Sbjct: 127 GLLDLGYVGFYAVGAYTYALCNQYFGLTFWECVPLAAAAS----ALFGFLLGFPVLRLRG 182 Query: 94 VYLAMATLGFGEVVRVVVLNM-EITGGPMGLNGVP------------PVTE--------- 131 YLA+ TLGFGE++R+++ NM ITGGP G++G+P PVTE Sbjct: 183 DYLAIVTLGFGEIIRLLLNNMTSITGGPDGVSGIPKPTVFGLVMARNPVTEGGTTFHQLF 242 Query: 132 ------------WWHIVLLLGVTVYFL-ARVRRSKIGRAFEAIKEDEVAARLMGVNVAGY 178 + + LL+ + YF+ +R+ R +GRA+EA++EDE+A R +G+N Sbjct: 243 GLSYQGGHMVIFLYFLALLMVLFTYFVTSRLLRMPMGRAWEALREDEIACRSLGINPTKV 302 Query: 179 KLLAFVIGAAIAGVAGALNAHYTFTIGPNNYGFENAVDILTMAVFGGTSNLIGPMIGSTI 238 KL AF +GAA AG+AG+ A + P ++ F + IL + V GG + +G ++ + + Sbjct: 303 KLSAFTLGAAFAGLAGSFFAARQGLVTPESFTFIESALILAIVVLGGMGSQLGVILAAIL 362 Query: 239 LSLLPEVLRHFKDFRLAANGLILILVVLYLPKGIWDPRR 277 L++LPE+ R F ++R+ GL+++L++++ P+G+ R Sbjct: 363 LTVLPELARSFAEYRMLIFGLVMVLMMMWRPQGLLPATR 401 >gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 138 bits (348), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 106/334 (31%), Positives = 159/334 (47%), Gaps = 82/334 (24%) Query: 26 MLALSIYVTLS-CGLLSLANAAFMGIGAYVASLIT------------------------- 59 MLAL + V + GLL L AF IGAY A L+ Sbjct: 52 MLALGLNVVVGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWL 111 Query: 60 -----MQAGLPFPVALAA--GGVLPALVALLIGIPTLRLSGVYLAMATLGFGEVVRVVVL 112 +Q G+ + L L AL L+G PTL+L G YLA+ TLGFGE++R+ + Sbjct: 112 CGPEIVQNGIHLSLWLIVPISAFLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMN 171 Query: 113 NME----ITGGPMGLN---------------------------GVPPVTEWWHIVLLLGV 141 N+ IT GP G+N +P V ++ + LLL + Sbjct: 172 NLNAPVNITNGPQGINLIDPIKVFGVSLAGEPGSGSMVKVFGMSMPSVNAYYFLFLLLCI 231 Query: 142 -TVYFLARVRRSKIGRAFEAIKEDEVAARLMGVNVAGYKLLAFVIGAAIAGVAGALNAHY 200 ++F R++ S++GRA+ AI+EDE+AA+ MG+N KLLAF +GA+ GVAGA+ + Sbjct: 232 GVIFFSVRLQDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAF 291 Query: 201 TFTIGPNNYGFENAVDILTMAVFGGTSNLIGPMIGSTILSLLPEVLRHFKD--------- 251 + P ++ ++ +L M V GG ++ G ++G IL+ LPEVLRH + Sbjct: 292 QGFVSPESFSLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGK 351 Query: 252 -------FRLAANGLILILVVLYLPKGIW-DPRR 277 R GL +++++L P G+W PR Sbjct: 352 VWIDAEVLRQLLYGLAMVVIMLTRPAGLWPSPRH 385 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 138 bits (347), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 79/231 (34%), Positives = 136/231 (58%), Gaps = 10/231 (4%) Query: 37 CGLLSLANAAFMGIGAYVASLITMQAGLPFPVALAAGGVLPALVALLIGIPTLRLSGVYL 96 G +SL +A GIG+Y A ++ + G+P V + A + A+ ++ +P LR++G YL Sbjct: 52 TGQVSLGHAGLFGIGSYAAGVLFFKLGMPIFVTVPAAIAVTAVFGAILALPALRVTGPYL 111 Query: 97 AMATLGFGEVVRVVVLNME-ITGGPMGLNGVPP---------VTEWWHIVLLLGVTVYFL 146 AM TL FG ++++++ M +T GP+G+ P V ++ + +L+ +++ + Sbjct: 112 AMVTLAFGTIIQILINEMTFLTEGPLGIKLQKPMIGGDKMSEVQFFYLVAVLMVLSMLVV 171 Query: 147 ARVRRSKIGRAFEAIKEDEVAARLMGVNVAGYKLLAFVIGAAIAGVAGALNAHYTFTIGP 206 R+ +S +GRAF+A+++ +A+ MGV+V YK+ AF+I AA+AG++GAL A+ I P Sbjct: 172 HRILKSNLGRAFQALRDSPIASDCMGVSVYRYKVYAFIISAALAGLSGALYAYSEEYISP 231 Query: 207 NNYGFENAVDILTMAVFGGTSNLIGPMIGSTILSLLPEVLRHFKDFRLAAN 257 N Y FE + L + GG + IG +IG+ I+ +LP +L FR A Sbjct: 232 NTYNFELTIMFLLAVIMGGRKSRIGSLIGALIVVMLPSLLSDIALFRQIAT 282 >gi|300310522|ref|YP_003774614.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 132 bits (331), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 81/240 (33%), Positives = 127/240 (52%), Gaps = 49/240 (20%) Query: 87 PTLRLSGVYLAMATLGFGEVVRVVVLNME----ITGGPMGLN------------------ 124 PTL+L G YLA+ TLGFGE++R+ + N+ IT GP G+N Sbjct: 146 PTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNITNGPQGINLIDPIKVFGVSLAGEPGS 205 Query: 125 ---------GVPPVTEWWHIVLLLGV-TVYFLARVRRSKIGRAFEAIKEDEVAARLMGVN 174 +P V ++ + LLL + ++F R++ S++GRA+ AI+EDE+AA+ MG+N Sbjct: 206 GSMVKVFGMSMPSVNAYYFLFLLLCIGVIFFSVRLQDSRLGRAWVAIREDEIAAKAMGIN 265 Query: 175 VAGYKLLAFVIGAAIAGVAGALNAHYTFTIGPNNYGFENAVDILTMAVFGGTSNLIGPMI 234 KLLAF +GA+ GVAGA+ + + P ++ ++ +L M V GG ++ G ++ Sbjct: 266 TRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESFSLTESIAVLAMVVLGGIGHIPGVVL 325 Query: 235 GSTILSLLPEVLRHFKD----------------FRLAANGLILILVVLYLPKGIW-DPRR 277 G IL+ LPEVLRH + R GL +++++L P G+W PR Sbjct: 326 GGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLRQLLYGLAMVVIMLTRPAGLWPSPRH 385 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.141 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 745,881 Number of extensions: 27203 Number of successful extensions: 213 Number of sequences better than 1.0e-08: 28 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 28 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)