BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0025.0100.p
         (577 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313821|ref|YP_003777913.1| histidine ammonia-lyase [Herbas...   776   0.0  
gi|300313003|ref|YP_003777095.1| histidine ammonia-lyase [Herbas...   172   6e-44

>gi|300313821|ref|YP_003777913.1| histidine ammonia-lyase
           [Herbaspirillum seropedicae SmR1]
          Length = 542

 Score =  776 bits (2005), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 385/428 (89%), Positives = 400/428 (93%), Gaps = 2/428 (0%)

Query: 1   MLLRANAFASNYSAPRVEVVDRLLDFINAGIHPIMPQKGSVGASGDLAPLAYLAAAIVGF 60
           MLLRANAFASNYSAPRVEVVDRLL FINAGIHPIMPQKGSVGASGDLAPLAYLAAAI GF
Sbjct: 104 MLLRANAFASNYSAPRVEVVDRLLAFINAGIHPIMPQKGSVGASGDLAPLAYLAAAIAGF 163

Query: 61  DEAEVMYQGKRMSAPEAITKAGIGPVKFDLKAKDASALINGCTVSLAVAVLAAHDSRNLL 120
           DEAEVMYQG+RM A EAI +AGIGPVKFDLKAKDASALINGCT SLAVA+LAAHD+R+LL
Sbjct: 164 DEAEVMYQGQRMRATEAIEQAGIGPVKFDLKAKDASALINGCTASLAVAILAAHDARHLL 223

Query: 121 TDACLSLGLTLEAMRAEMSAFDPRIQQARPHAGQIKTAAVIRTLLKGSTRTTHEARSVQL 180
           TDACLSLGLTLEAMRAEMSAFDPRIQQARPHAGQIKTA VIRTLL+GSTRTTH AR+VQL
Sbjct: 224 TDACLSLGLTLEAMRAEMSAFDPRIQQARPHAGQIKTAEVIRTLLQGSTRTTHAARAVQL 283

Query: 181 PDESRRTDIPYSSRIQDVYSLRCAPQVYGPVFDALDYIDTIIDKEINSATDNPLIFSKEE 240
           PDE RRTDI Y++RIQDVYSLRCAPQVYGPVFDALDYIDTII+KE NSATDNPLIF KEE
Sbjct: 284 PDELRRTDIAYTARIQDVYSLRCAPQVYGPVFDALDYIDTIIEKETNSATDNPLIFHKEE 343

Query: 241 ADGGGFEIISGGNFHGQYLAQAMDLLAMTITDLGSICERRIARLIDPTLSWGLPRNLMSG 300
             G GFEIISGGNFHGQYLAQAMDLLAM ITDLGSICERRIARLIDPTLSWGLPRNLMSG
Sbjct: 344 --GEGFEIISGGNFHGQYLAQAMDLLAMAITDLGSICERRIARLIDPTLSWGLPRNLMSG 401

Query: 301 VRGVNTGYPVVQCSMSSLVMENRTLSMPGSVDSIPSKGNSEDHVSNSTWCARKAATVVAN 360
           VRGVNTGYPVVQCSMSSLVMENRTL MPGSVDSIPSKGNSEDH+SNSTWCARKA TVV N
Sbjct: 402 VRGVNTGYPVVQCSMSSLVMENRTLCMPGSVDSIPSKGNSEDHISNSTWCARKAMTVVQN 461

Query: 361 TQYIVGVEMLLAAQGLTMTEELLKDFVLGEGTQAAYEEIRKQIPACLDGDRWFHNDIQVA 420
           TQYIVGVEMLLAAQ LTMTE LLKDFVLG+GTQAAY EIR+QIPACLDGDRWFH+D+Q A
Sbjct: 462 TQYIVGVEMLLAAQALTMTEALLKDFVLGQGTQAAYAEIRRQIPACLDGDRWFHDDVQAA 521

Query: 421 HXVSASSS 428
           H    S S
Sbjct: 522 HGFVVSGS 529


>gi|300313003|ref|YP_003777095.1| histidine ammonia-lyase
           [Herbaspirillum seropedicae SmR1]
          Length = 526

 Score =  172 bits (437), Expect = 6e-44,   Method: Compositional matrix adjust.
 Identities = 121/377 (32%), Positives = 196/377 (51%), Gaps = 33/377 (8%)

Query: 4   RANAFASNYSAPRVEVVDRLLDFINAGIHPIMPQKGSVGASGDLAPLAYLAAAIVGFDEA 63
           R N+    +S   V +++++   +   + P +P +GSVGASGDL PL+YLAA + G  E 
Sbjct: 117 RLNSLCQGFSGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPLSYLAAVLCG--ER 174

Query: 64  EVMYQGKRMSAPEAITKAGIGPVKFDLKAKDASALINGCTVSLAVAVLAAHDSRNLLTDA 123
           +V  +G  + A +A+  AG+ P++  L+ K+  A++NG  V  A+A LA   +R L   A
Sbjct: 175 DVWREGATVPAAQALAAAGMTPLR--LRPKEGLAIMNGTAVMTALACLAFDRARYLCQLA 232

Query: 124 CLSLGLTLEAMRAEMSAFDPRIQQARPHAGQIKTAAVIRTLLKGSTRTTHEARSVQLPDE 183
                ++   +      FD  +  A+PH GQ + AA ++  L       +E         
Sbjct: 233 TRITAMSSFTLDGNAHHFDATLFSAKPHPGQQQVAAWLQRDLPCGQAHRNE--------- 283

Query: 184 SRRTDIPYSSRIQDVYSLRCAPQVYGPVFDALDYIDTIIDKEINSATDNPLIFSKEEADG 243
                     R+QD YS+RCAP V G + DAL  +   I+ E+NSA DNPLI      D 
Sbjct: 284 ---------KRLQDRYSVRCAPHVIGVLNDALPSLRQFIENELNSANDNPLI------DP 328

Query: 244 GGFEIISGGNFHGQYLAQAMDLLAMTITDLGSICERRIARLIDPTLSWGLPRNLMSGVRG 303
            G  ++ GG+F+G ++A AMD +   + ++  + +R++A ++D   + GLP NL SG +G
Sbjct: 329 DGERVLHGGHFYGGHIAFAMDSMKTAVANVADLLDRQMALMVDQRYNNGLPANL-SGAQG 387

Query: 304 ----VNTGYPVVQCSMSSLVMENRTLSMPGSVDSIPSKGNSEDHVSNSTWCARKAATVVA 359
               +N G   +Q S S+   E   L+MP SV S  ++ +++D VS  T  AR    V+ 
Sbjct: 388 ARAPINHGLKALQISASAWTAEALKLTMPASVFSRSTECHNQDKVSMGTIAARDCLRVLE 447

Query: 360 NTQYIVGVEMLLAAQGL 376
            T+ +    ++   QG+
Sbjct: 448 LTEQVAAALLITVRQGV 464


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.132    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,679,423
Number of extensions: 65970
Number of successful extensions: 194
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 190
Number of HSP's successfully gapped: 2
Length of query: 577
Length of database: 1,619,870
Length adjustment: 90
Effective length of query: 487
Effective length of database: 1,193,720
Effective search space: 581341640
Effective search space used: 581341640
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 133 (55.8 bits)