BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0098.p (202 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313544|ref|YP_003777636.1| transmembrane protein [Herbaspi... 192 2e-50 >gi|300313544|ref|YP_003777636.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 192 bits (487), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 92/179 (51%), Positives = 127/179 (70%), Gaps = 5/179 (2%) Query: 7 SYGVEWLNLLVRWLHLIAGIAWIGASFYFVWLDNSIRPPKPGSDLAKKGVSGELWAVHGG 66 +Y ++WLNLL+RWLH+I IAWIG+SFYFVWLDNS+ P +L +KGV GELWAVHGG Sbjct: 3 AYILDWLNLLLRWLHVITAIAWIGSSFYFVWLDNSLVAPT-DPELKEKGVGGELWAVHGG 61 Query: 67 GFYNPQKYLVAPSELPEELHWFKWEAYATWISGFAMLFIVYYFNASAMMVDKSVADLTGL 126 GFYNPQKYL+AP LPE LHWF WE+Y+TW+SGFA+ ++Y FNA+ +VD++V D++ Sbjct: 62 GFYNPQKYLLAPKVLPEHLHWFYWESYSTWLSGFALFSLLYLFNANVFLVDRNVFDMSAS 121 Query: 127 QSVGVGLGTLVLGWIVYDLLCKSPLGKREG---LLGAVMFVFIVAVAYVLTHLLSGHAG 182 + G + L+ W++YD +C S +G R G L+ ++ + +V + HL SG A Sbjct: 122 MAGGAAIAFLIFNWLIYDAIC-STIGDRPGADRLVNLLVGLQVVVSVWAACHLFSGRAA 179 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.474 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 633,280 Number of extensions: 25282 Number of successful extensions: 95 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 94 Number of HSP's successfully gapped: 1 Length of query: 202 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 121 Effective length of database: 1,236,335 Effective search space: 149596535 Effective search space used: 149596535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)