BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0094.p (506 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase... 425 e-120 gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidoh... 235 8e-63 gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum ... 129 5e-31 >gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase/N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 587 Score = 425 bits (1092), Expect = e-120, Method: Compositional matrix adjust. Identities = 206/265 (77%), Positives = 227/265 (85%), Gaps = 3/265 (1%) Query: 3 TLEQLNGSAARDVPAFVNTLHGIYEHSPWIPERAAKLGPFANITALKQALQSVVTGADRA 62 TL+ LN ++A D F L GIYEHSPWI ERAA PF+ ITALK ALQS V A RA Sbjct: 4 TLQDLNQASASD---FTAALQGIYEHSPWIAERAAPQRPFSTITALKLALQSRVNQASRA 60 Query: 63 EQLGLIRAHPELAGKAAIAGELTAESTGEQAKAGLNLCTPEEFATLQKLNGDYNARFGFP 122 EQLGLIRAHPELAG+AAIAGELTAESTGEQAKAGL+LC+PEEFA LQ+LN DYNARFGFP Sbjct: 61 EQLGLIRAHPELAGRAAIAGELTAESTGEQAKAGLHLCSPEEFARLQQLNADYNARFGFP 120 Query: 123 FILAVKGATGKGLTRQQIITTFTRRLRNQPEDELREALRQIGRIAEMRINDLLGHTPAIG 182 FILAVKG TG GLTRQQII TFTRRLRNQ EDELRE+LRQIGRIAE+R+N+LLGH P +G Sbjct: 121 FILAVKGPTGAGLTRQQIIATFTRRLRNQVEDELRESLRQIGRIAEIRLNELLGHQPTLG 180 Query: 183 ATIMEWAEEIGAWSEDEGALTCTYMTDAHRRTADQIAHWMREAGMHAHIDAVGNVVGRYL 242 AT+M+WAEEIGA SE+EGALTCTY+T+ H+RTA QIA WMREAGM HIDAVGNVVGRY Sbjct: 181 ATVMQWAEEIGALSEEEGALTCTYLTETHQRTAAQIAQWMREAGMQVHIDAVGNVVGRYQ 240 Query: 243 STDPQAKTLMTGSHYDTVRNGGVYE 267 S PQAKTL+TGSHYDTVRNGG Y+ Sbjct: 241 SPHPQAKTLLTGSHYDTVRNGGKYD 265 Score = 411 bits (1056), Expect = e-116, Method: Compositional matrix adjust. Identities = 200/238 (84%), Positives = 220/238 (92%) Query: 269 IARDPSDLLGYVEVHIEQGPVLLNRDLPVGIVTSIAGSSRYLVNLKGVASHAGTTPMNMR 328 IARDP+++L YVEVHIEQGPVLL RDLPVGIVTSIAGSSRYLV+LKGVASHAGTTPM+MR Sbjct: 350 IARDPAEILAYVEVHIEQGPVLLKRDLPVGIVTSIAGSSRYLVDLKGVASHAGTTPMDMR 409 Query: 329 KDAAAAAAEIILYVEQRCGEDQHASLVGTVGQLQVPNGSTNVIPGACTLSLDIRAAADDV 388 KDAAAAAAEIILYVEQRC +DQHA+LVGTVGQLQVP GSTNVIPGAC LSLDIRAA D+V Sbjct: 410 KDAAAAAAEIILYVEQRCSQDQHAALVGTVGQLQVPRGSTNVIPGACQLSLDIRAATDEV 469 Query: 389 RDAAVADVLKKIEEICQRRSVDVTIEKTVSAPAAPCARWLMNQLAAATERAGVKPFELAS 448 R AAV D+L KIEEIC RRS+D+ +E+T+SAPAAPCA WLM QL+AAT+RAGV PFELAS Sbjct: 470 RLAAVKDILAKIEEICTRRSIDLKLEETLSAPAAPCAPWLMAQLSAATQRAGVPPFELAS 529 Query: 449 GAGHDAMTIAKMTDVAMLFTRCGNGGISHNPLETMTADDAEVSAQILLDFLRNFKAKV 506 GAGHDAM + ++TDV MLFTRCGNGGISHNPLETMTADDAEVS QILLDFLRNFKAK+ Sbjct: 530 GAGHDAMAMVRLTDVCMLFTRCGNGGISHNPLETMTADDAEVSGQILLDFLRNFKAKI 587 >gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 235 bits (599), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 123/236 (52%), Positives = 160/236 (67%), Gaps = 2/236 (0%) Query: 268 AIARDPSDLLGYVEVHIEQGPVLLNRDLPVGIVTSIAGSSRYLVNLKGVASHAGTTPMNM 327 A A P + +VEVHIEQGP+LLN DLPVG+VT+I+G+SR++ + G+A HAGT PM++ Sbjct: 183 ADAWQPGSMAAFVEVHIEQGPLLLNEDLPVGVVTAISGASRFMAEVHGLAGHAGTVPMHL 242 Query: 328 RKDAAAAAAEIILYVEQRCGEDQHASLVGTVGQLQVPNGSTNVIPGACTLSLDIRAAADD 387 R+DAA AAAEI LY+E+RC LVGT+G L+V G+ NV+PG + S+DIRA D Sbjct: 243 RRDAAMAAAEIGLYIERRC--SIKPELVGTMGLLEVVQGAANVVPGLASFSIDIRAEDDS 300 Query: 388 VRDAAVADVLKKIEEICQRRSVDVTIEKTVSAPAAPCARWLMNQLAAATERAGVKPFELA 447 R AAVADV +I+ I RR V + + +T A + PCA L LA + AG L Sbjct: 301 DRLAAVADVKAEIDRIAARRQVQLALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLP 360 Query: 448 SGAGHDAMTIAKMTDVAMLFTRCGNGGISHNPLETMTADDAEVSAQILLDFLRNFK 503 SGAGHDAM +A + DVAMLF RCGNGGISH+P ETMT DA ++AQ+ + F+ Sbjct: 361 SGAGHDAMALAGLADVAMLFVRCGNGGISHHPDETMTEADAAIAAQVFSHLVEQFQ 416 Score = 79.3 bits (194), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 40/88 (45%), Positives = 54/88 (61%), Gaps = 2/88 (2%) Query: 182 GATIMEWAEEIGAWSEDEGALTCTYMTDAHRRTADQIAHWMREAGMHAHIDAVGNVVGRY 241 G M+WA+++ +E G LT TY+T H+ A +A WM EAGM D GNV+GRY Sbjct: 12 GRQAMQWAQQLAQHTESPGMLTRTYLTPQHQAAAAALARWMEEAGMRVRRDNAGNVIGRY 71 Query: 242 LST--DPQAKTLMTGSHYDTVRNGGVYE 267 + A + +TGSH+DTVRNGG Y+ Sbjct: 72 EAAPGHESAPSFVTGSHFDTVRNGGWYD 99 >gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 129 bits (325), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 77/221 (34%), Positives = 116/221 (52%), Gaps = 8/221 (3%) Query: 279 YVEVHIEQGPVLLNRDLPVGIVTSIAGSSRYLVNLKGVASHAGTTPMNMRKDAAAAAAEI 338 Y E HIEQGPVL N D +G+V ++ G S Y + G SHAG TPM +RKDA ++ Sbjct: 192 YYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEIRKDAL----QV 247 Query: 339 ILYVEQRCGEDQHASLV---GTVGQLQVPNGSTNVIPGACTLSLDIRAAADDVRDAAVAD 395 Y+ Q H GTVGQ+QV S NVIPG S+D+R ++ D Sbjct: 248 STYIMQEVVAIAHRYAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEASLTRMHED 307 Query: 396 VLKKIEEICQRRSVDVTIEKTVSAPAAPCARWLMNQLAAATERAGVKPFELASGAGHDAM 455 +L +E + ++ + + +E+ P P ++++ AA+ G K ++ SGAGHDA+ Sbjct: 308 LLAYLEAMRKKTGLSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVSGAGHDAI 367 Query: 456 TIAKMTDVAMLFTRCGNGGISHNPLETMTADDAEVSAQILL 496 A++ M+F C + G+SHN +E + E +LL Sbjct: 368 YAARLAPAGMIFVPCKD-GVSHNEIEDARPEHLEAGCNVLL 407 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,523,624 Number of extensions: 62106 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 5 Length of query: 506 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 417 Effective length of database: 1,198,455 Effective search space: 499755735 Effective search space used: 499755735 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)