BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0093.p (486 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 328 6e-91 gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 136 3e-33 gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirill... 60 3e-10 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 328 bits (841), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 151/168 (89%), Positives = 159/168 (94%) Query: 1 MSVGQSKGGLPPDRVVEDENDILRDTQRLIETYHDHSRHAMQRIVVAPCSPFSVSRDLMR 60 MSVGQSKGGLPPDRVV+DE IL+DTQRLIETYHDH RHAMQRIVVAPCSPFSVSR+LMR Sbjct: 163 MSVGQSKGGLPPDRVVQDEAAILKDTQRLIETYHDHQRHAMQRIVVAPCSPFSVSRELMR 222 Query: 61 EAANMARSYKVSLHTHLAENVNDIAYSREKFNMTPAEYAEDCGWVGHDVWHAHCVQLDDH 120 EAA++AR Y VSLHTHLAENVNDIAYSREKFNM+PAEYAEDCGWVGHDVWHAHCVQLD H Sbjct: 223 EAADLARHYGVSLHTHLAENVNDIAYSREKFNMSPAEYAEDCGWVGHDVWHAHCVQLDAH 282 Query: 121 GIDLFARTGTGVAHCPCSNMRLASGIAPIRKMIAAGVPVGLGVDXIAA 168 GIDLFARTGTGVAHCPCSNMRLASGIAPIRKM+ AGVPVGLGVD A+ Sbjct: 283 GIDLFARTGTGVAHCPCSNMRLASGIAPIRKMLDAGVPVGLGVDGSAS 330 Score = 289 bits (739), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 138/176 (78%), Positives = 156/176 (88%), Gaps = 2/176 (1%) Query: 189 KTLLIKNATVVVTMNDTREEIKNGALFIRNNIIEQVGQTADLPQTADEIIDASNHVVLPG 248 KTLLI+NA ++VTMN++REEI +GAL+IRNN+IEQVGQTADLPQ ADEIIDA +H+VLPG Sbjct: 8 KTLLIRNARILVTMNESREEIPDGALYIRNNVIEQVGQTADLPQDADEIIDARHHIVLPG 67 Query: 249 LINTHHHMYQSLTRVIPAAQDGELFNWLTNLYPIWAGLTPEMIKVSTLTAMAELILSGCT 308 LINTHHHMYQSLTR IP+AQ+GELFNWLT LYPIWAGLTPEMI+VSTLTAMAELILSGCT Sbjct: 68 LINTHHHMYQSLTRAIPSAQNGELFNWLTKLYPIWAGLTPEMIQVSTLTAMAELILSGCT 127 Query: 309 TSSDHLYIYPNKTRLDDSLEAAQEIGMRFHGARGAAX--KAKVGLGVDGSASNDGA 362 TSSDHLYIYPN+TRLDDSLEAA IGMRFHGARGA ++K GL D ++ A Sbjct: 128 TSSDHLYIYPNQTRLDDSLEAAARIGMRFHGARGAMSVGQSKGGLPPDRVVQDEAA 183 Score = 235 bits (599), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 116/138 (84%), Positives = 123/138 (89%) Query: 349 VGLGVDGSASNDGAHMLGEVRQAMLLQRVGFGPDAMTARQALEIATLGGAKVLNRDDIGA 408 VGLGVDGSASND AHMLGEVRQAMLLQRVGFGPDAMTARQALE+ATLGGAKVLNRDDIGA Sbjct: 321 VGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAMTARQALEVATLGGAKVLNRDDIGA 380 Query: 409 LKPGMSADVAMFKLDQIGFAGALHDPVAALVFCTPANVSYSIINGRVIVREGQCTTIDLG 468 LKPGMSADV MF QI FAGALHDPVAALVFCTPANV SIINGRVIVREGQ T+I+LG Sbjct: 381 LKPGMSADVVMFDTRQIAFAGALHDPVAALVFCTPANVDCSIINGRVIVREGQLTSIELG 440 Query: 469 SVIERHNALAFELADAAN 486 +V+ERHN L+ LA + Sbjct: 441 AVLERHNQLSLALAQGVD 458 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 136 bits (343), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 71/153 (46%), Positives = 93/153 (60%), Gaps = 1/153 (0%) Query: 17 EDENDILRDTQRLIETYHDHSRHAMQRIVVAPCSP-FSVSRDLMREAANMARSYKVSLHT 75 E + L D Q L +HD + +M+R+V+AP +P +S + D +RE A AR + LH+ Sbjct: 177 ETLDGFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELREMAVAAREMGLRLHS 236 Query: 76 HLAENVNDIAYSREKFNMTPAEYAEDCGWVGHDVWHAHCVQLDDHGIDLFARTGTGVAHC 135 HL+E V + P E+ E GW+G DVW AH V+LD I L TGTG+AHC Sbjct: 237 HLSETVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHC 296 Query: 136 PCSNMRLASGIAPIRKMIAAGVPVGLGVDXIAA 168 P SN RL SGIAPIR + AAGVPV +GVD A+ Sbjct: 297 PQSNGRLGSGIAPIRALEAAGVPVSIGVDGAAS 329 Score = 89.0 bits (219), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 53/165 (32%), Positives = 83/165 (50%), Gaps = 7/165 (4%) Query: 189 KTLLIKNATVVVT-MNDTREEIKNGALFIRNNIIEQVGQTADLPQTADEIIDASNHVVLP 247 T LI+N ++T + + + + +I +GQ LP +E+IDAS+ V P Sbjct: 2 STFLIRNLQAIMTGLPGAAARHAGPDIRVSDGVIAAIGQLDALP--GEELIDASHCVAYP 59 Query: 248 GLINTHHHMYQSLTRVIPAAQDGELFNWL-TNLYPIWAGLTPEMIKVSTLTAMAELILSG 306 +NTHHH++QSL + P + L WL Y A ++ +++ M EL+ SG Sbjct: 60 AWVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSG 119 Query: 307 CTTSSDHLYIYPNKTRLDDS---LEAAQEIGMRFHGARGAAXKAK 348 C T +DH Y+Y D S E AQ++G+RF RG A + + Sbjct: 120 CGTIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTR 164 Score = 66.6 bits (161), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 48/143 (33%), Positives = 68/143 (47%), Gaps = 17/143 (11%) Query: 344 AXKAKVGLGVDGSASNDGAHMLGEVRQAMLLQRV--------GF-------GPDAMTARQ 388 A V +GVDG+ASN+ A M+ E A L+QR F G DA T Sbjct: 315 AAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFRGGHFEGGADAATVED 374 Query: 389 ALEIATLGGAKVLNRDDIGALKPGMSADVAMFKLDQIGFAGALHDPVAALVFCTPANVSY 448 + T GGA+VL D + L+ G AD+A+++LD + G LHDP V Sbjct: 375 VVRWGTTGGARVLGLDALDGLQVGQQADIALYRLDDPRYFG-LHDPAIGPVASGGRPFLK 433 Query: 449 SI-INGRVIVREGQCTTIDLGSV 470 ++ + GR +VRE +DL + Sbjct: 434 AMWVGGRAVVREDAIPGVDLAEL 456 >gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirillum seropedicae SmR1] Length = 432 Score = 60.5 bits (145), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 41/157 (26%), Positives = 75/157 (47%), Gaps = 8/157 (5%) Query: 12 PDRVVEDENDILRDTQRLIETYHDHSR-HAMQRIVVAPCSPFSVSRDLMREAANMARSY- 69 PD + + +R+++ LI +H+ R H + AP S + + M+ A +A Y Sbjct: 169 PDFLRDTAESGVRESEALIGRWHNKGRQHYAITVRFAPTS----THEQMKLAGELAAQYP 224 Query: 70 KVSLHTHLAENVNDIAYSREKF--NMTPAEYAEDCGWVGHDVWHAHCVQLDDHGIDLFAR 127 + TH+AEN +++ + +E + + + + G + +AHC+ LDD A Sbjct: 225 DTYIQTHVAENTDEVKWVKELYPDARSYLDVYDGYGLMRQRALYAHCIWLDDQDRARMAA 284 Query: 128 TGTGVAHCPCSNMRLASGIAPIRKMIAAGVPVGLGVD 164 T + CP SN+ L SG+ + AA +P+ + D Sbjct: 285 TQSAATLCPTSNLFLGSGLFDFARADAARLPLSVATD 321 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,459,454 Number of extensions: 58979 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 7 Length of query: 486 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 397 Effective length of database: 1,198,455 Effective search space: 475786635 Effective search space used: 475786635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)