BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0087.p (295 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 149 3e-37 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 120 1e-28 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 113 2e-26 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 84 2e-17 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 149 bits (375), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 105/288 (36%), Positives = 153/288 (53%), Gaps = 10/288 (3%) Query: 2 AKVGFIGLGIMGSPMAENLQKGGHKLYLHDQ-KNPPAALIE-GGATVCTSGAEVAKRADI 59 A + FIGLG MG PMA+NL K G + HD K L+E GG T S A VAK AD+ Sbjct: 3 ANIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAK-ADV 61 Query: 60 IIVMVPDTPHVEAVLFSKGGVAEGLSAGKIVVDMSSISPIATKGFAQKINALGCEYLDAP 119 +I M+P + HVE++ + GV G +++D S+I+P A + A A G E LDAP Sbjct: 62 VITMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAP 121 Query: 120 VSGGEVGAKAASLTIMVGGNESAFNDVKSLFELMGKNITLVGGNGDGQTTKVANQIIVAL 179 VSGG GA A +LT MVGG++ AF + + MGK I G +G GQT KV N +++ + Sbjct: 122 VSGGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGI 181 Query: 180 NIQAVAEALLFASKAGADPARVRQALMGGFASSRILEVHG-------ERMVKRTFNPGFR 232 + +EA+ G DP + + + + LEV+ + + GF Sbjct: 182 LMIGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGFG 241 Query: 233 IELHQKDLNLALQGAKALGVSLPNTAATQELMNACAANGMSGLDHSAL 280 ++L KDL LA++ A A ++P A + L + + NG LD S++ Sbjct: 242 VDLMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSI 289 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 120 bits (301), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 79/246 (32%), Positives = 140/246 (56%), Gaps = 2/246 (0%) Query: 1 MAKVGFIGLGIMGSPMAENLQKGGHKLYLHD-QKNPPAALIEGGATVCTSGAEVAKRADI 59 M K+G IGLG MG MA +LQ+GG+++ +D Q++ AL + G S A +A ++ Sbjct: 1 MKKIGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEM 60 Query: 60 IIVMVPDTPHVEAVLFSKGGVAEGLSAGKIVVDMSSISPIATKGFAQKINALGCEYLDAP 119 +++ +P + VE V+ +GG+ + + G ++VD ++ P +T+ A ++ A G E +DAP Sbjct: 61 VVLSLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAP 120 Query: 120 VSGGEVGAKAASLTIMVGGNESAFNDVKSLFELMGKNITLVGGNGDGQTTKVANQIIVAL 179 VSGG GA A++ +++GG+ ++ + +M VG G G K+ N ++ Sbjct: 121 VSGGPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVTGT 180 Query: 180 NIQAVAEALLFASKAGADPARVRQALMGGFASSRILEV-HGERMVKRTFNPGFRIELHQK 238 ++ EA A+ AG D ++ Q L G A+S+++EV + + F+ GF ++L +K Sbjct: 181 HLLIAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKLMRK 240 Query: 239 DLNLAL 244 DL LA+ Sbjct: 241 DLRLAM 246 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 113 bits (282), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 76/281 (27%), Positives = 133/281 (47%), Gaps = 2/281 (0%) Query: 4 VGFIGLGIMGSPMAENLQKGGHKLYLHD-QKNPPAALIEGGATVCTSGAEVAKRADIIIV 62 VG IGLG MG +A +L + G ++ D +K G C + AE+A + D++I Sbjct: 5 VGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVIT 64 Query: 63 MVPDTPHVEAVLFSKGGVAEGLSAGKIVVDMSSISPIATKGFAQKINALGCEYLDAPVSG 122 +V + E VLF G + G +V+ ++++P +++ G LDAPVSG Sbjct: 65 LVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAPVSG 124 Query: 123 GEVGAKAASLTIMVGGNESAFNDVKSLFELM-GKNITLVGGNGDGQTTKVANQIIVALNI 181 G A + +T+M G + ++ + M GK L +G G K+ NQ++ ++I Sbjct: 125 GAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHI 184 Query: 182 QAVAEALLFASKAGADPARVRQALMGGFASSRILEVHGERMVKRTFNPGFRIELHQKDLN 241 A AEA+ + G +P + + + +S + E ++ + P +++ KDL Sbjct: 185 AASAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFVKDLG 244 Query: 242 LALQGAKALGVSLPNTAATQELMNACAANGMSGLDHSALCR 282 L L A+ LP +AA ++ + G G D SA+ + Sbjct: 245 LVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIK 285 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 90/285 (31%), Positives = 137/285 (48%), Gaps = 6/285 (2%) Query: 3 KVGFIGLGIMGSPMAENLQKGGHKLYLHDQKNPPAA-LIEGGATVCTSGAEVAKRADIII 61 K+ F+GLG MG PMA NL + G+ + ++ P L GA + AE A AD++I Sbjct: 2 KIAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADADVLI 61 Query: 62 VMVPDTPHVEAVLFSKGGVAEGLSAGKIVVDMSSISPIATKGFAQKINALGCEYLDAPVS 121 M+ D E L G L G I V+M++IS A A G Y+ APV Sbjct: 62 SMLADDDATETSL-LDAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVL 120 Query: 122 GGEVGAKAASLTIMVGGNESAFNDVKSLFELMGKNITLVGGNGD-GQTTKVANQIIVALN 180 G A+A L I+ G E A V+ L +++G+ +G + K+A ++A Sbjct: 121 GRVNVAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIASA 180 Query: 181 IQAVAEALLFASKAGADPA-RVRQALMGGFASSRILEVHGERMVKRTFNP-GFRIELHQK 238 I A++EA+ G D A + A FA+ + + +G+ + F P GF++ L K Sbjct: 181 IGAMSEAVALVQGYGLDKAGFIELATSTAFAAP-VYKGYGQAIADDRFEPAGFKLALGLK 239 Query: 239 DLNLALQGAKALGVSLPNTAATQELMNACAANGMSGLDHSALCRA 283 D+ LAL+ + V L +A ++L A+G LD +AL RA Sbjct: 240 DVRLALEAGEQAHVPLSLASALRDLHIDGLAHGEGHLDWAALSRA 284 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 915,064 Number of extensions: 37750 Number of successful extensions: 196 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 4 Length of query: 295 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 210 Effective length of database: 1,217,395 Effective search space: 255652950 Effective search space used: 255652950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)