BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0025.0086.p
         (259 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313515|ref|YP_003777607.1| hydroxypyruvate isomerase [Herb...   241   4e-65

>gi|300313515|ref|YP_003777607.1| hydroxypyruvate isomerase
           [Herbaspirillum seropedicae SmR1]
          Length = 267

 Score =  241 bits (615), Expect = 4e-65,   Method: Compositional matrix adjust.
 Identities = 114/253 (45%), Positives = 160/253 (63%)

Query: 1   MTKLAANLTMLFTELPFLERFRAAAQANFKAVEFLFPYAFEPAQIAEQLRKNELELVLHN 60
           M + AANL+M++ E  FL+RF AAA+  F  VEFLFPY + P +I  +L  N L   L N
Sbjct: 1   MLRFAANLSMMYVEHDFLDRFAAAAKDGFSGVEFLFPYDYAPEEIKSRLEANGLTQALFN 60

Query: 61  LPAGNWEAGERGIACHPDRVSEFRQGIDDAIRYAKILGVRQLNCLVGIQPQGVSAAAARE 120
            P G+W AGERGIA  P R  EF++ I+ A++YA ++G + L+ + G+   G   A  R 
Sbjct: 61  APPGDWAAGERGIASLPGRQDEFKRSIETALKYAAVIGNQTLHVMAGLIKPGQDRAQHRA 120

Query: 121 TLVANLRFAADKLQAEGIALLIEPINYFDIPGFFLKTTKQAAELIASTGSSNLFIQYDIY 180
             + NL +AA + ++ G+ ++IEPIN  +IPGFFL     A  + A  G+ NL +Q+D Y
Sbjct: 121 VYLDNLSYAAHQAESAGLTVVIEPINTRNIPGFFLNRQDDAQAICAEVGAPNLKVQFDCY 180

Query: 181 HMQRMEGELANTIKANLSLIRHVQLADNPGRFEPGTGEINYRFLFKHLDEIGYQGWIGCE 240
           H Q +EG++A  +K ++  I H+Q+A  P R EP  GE+NY +L K +D +GYQGWIGCE
Sbjct: 181 HCQIVEGDVAVKLKRDMPGIGHIQIAGVPERHEPDVGELNYPYLLKLIDTLGYQGWIGCE 240

Query: 241 YKPKAGTVEGLGW 253
           Y P   T  GLGW
Sbjct: 241 YNPAGATSAGLGW 253


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.139    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 807,302
Number of extensions: 33180
Number of successful extensions: 85
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 85
Number of HSP's successfully gapped: 1
Length of query: 259
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 176
Effective length of database: 1,226,865
Effective search space: 215928240
Effective search space used: 215928240
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 129 (54.3 bits)