BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0085.p (393 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 146 3e-36 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 80 2e-16 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 57 2e-09 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 146 bits (368), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 102/349 (29%), Positives = 170/349 (48%), Gaps = 19/349 (5%) Query: 1 MLNQSERPLLTIGGGVVNADAAELAVEFAELTGVPVIPTLMAWGAIPDDHPQMAGMVGLQ 60 +L +ERP++ GGG++ A+AA + + G P TLM GA + GM G+ Sbjct: 202 LLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGLGAYRSSSDKFVGMPGMH 261 Query: 61 TSHRYGNATMLASDFVLGIGNRWANRHTGSIEVF-TKDRTFVHVDIEPTQIGRVFGPDYG 119 ++ N M D ++ IG R+ +R G+ + F + R +H+DI+P+ I + D Sbjct: 262 GTYE-ANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARKIIHIDIDPSSISKRVKVDIP 320 Query: 120 IVSDAKAALKLFIEVAKELKAAGNLPDRSAWLADCQE-RKRTLHRRTHFEEVPIKPQRVY 178 IV + K L+ + + N AW E RKR + EE IKPQ V Sbjct: 321 IVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRKRDCLKFATSEEF-IKPQSVV 379 Query: 179 EEMNRAFGRDTCYVSTIGLSQIAAAQFLHVYKDRHWINCGQAGPLGWTISAALGVCAADP 238 +++ D S +G Q+ AAQ+ K R WIN G G +G + A+GV A+P Sbjct: 380 QKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGGLGTMGVGLPYAMGVQMANP 439 Query: 239 QRKVVAISGDYDFQFMIEELAVGAQFNLPYLHVVVNNSYLGLIRQAQRGFEMDYCVQLAF 298 V I+G+ Q I+ELA Q++L +++NN +LG++RQ Q ++DY + + Sbjct: 440 DATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMVRQWQ---QIDYGSRYSE 496 Query: 299 ENINA-PELNGYGVDHVAVVEGLGCKAIRVTNPNEI----QDAFAVAQR 342 +++ P+ N + E G +++ P ++ ++AFA+ R Sbjct: 497 SYMDSLPDFN-------KLAESYGHVGMKIEKPGDVDGALREAFAMKDR 538 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 97/375 (25%), Positives = 155/375 (41%), Gaps = 29/375 (7%) Query: 1 MLNQSERPLLTIGGGVVNADAAELAVEFAELTGVPVIPTLMAWGAIPDDHPQMAGMVGLQ 60 +L Q+ +PLL GGGV+ +A E FAE GVPV T A+P H G +G+ Sbjct: 232 LLRQARQPLLIAGGGVLYGNACEHLRRFAEQHGVPVAETQAGKSALPWSHALQMGAIGV- 290 Query: 61 TSHRYGNATMLASDFVLGIGNRWANRHTGSIEVFTKDRTFVHVDIEPTQIGRVFGPDYGI 120 T NA +D V+ +G R + TGS +F + R +++++ + G + Sbjct: 291 TGSPAANALAQEADVVIAVGTRLQDFTTGSHTLFAQAR-LLNLNVNAMDAHKWRG--LAL 347 Query: 121 VSDAKAALKLFIEVAKELKAAGNLPDRSAWLA-DCQERKRTLHRRTHFEEVPIKPQRVYE 179 +DA L+ + ++A R+ LA D +ER + +T V Sbjct: 348 QADAGLGLEALSHALEGWRSAPEWHARAHTLAQDWRERVAQITGQTDTGGRLPYDGEVIG 407 Query: 180 EMNRAFGRD------TCYVSTIG--LSQIAAAQFLHVYKDRHWINCGQAGPLGWTISAAL 231 + R+ C T+ L ++ A Y + +C +G+ ++ L Sbjct: 408 AIQRSVADSPSQDIVVCAAGTLPAELHKLWRAGRPGAYHVEYGYSC-----MGYEVAGGL 462 Query: 232 GVCAADPQRKVVAISGDYDFQFMIEELAVGAQFNLPYLHVVVNNSYLGLIRQAQRGFEMD 291 GV A PQR+V+ I GD + M ELA + VV++N G I + Q+ Sbjct: 463 GVKLAQPQREVIVIVGDGSYLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQA---- 518 Query: 292 YCVQLAFENINAPELN-GYG---VDHVAVVEGLGCKAIRVTNPNEIQDAFAVAQRWMAEY 347 C F N+ A L G G +D A LG V E++ A A+ A+ Sbjct: 519 -CGGAPFNNMLADCLQAGPGAPAIDFAAHARSLGALGENVKTITELEAALQRAR--AADR 575 Query: 348 RVPVVVEIILERVTN 362 V ++ R T+ Sbjct: 576 SYLVCIDTDASRTTD 590 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 76/342 (22%), Positives = 123/342 (35%), Gaps = 40/342 (11%) Query: 35 PVIPTLMAWGAIPDDHPQMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSIEVF 94 PV+ T DHP G VGL G + A++ +L +G+ + E Sbjct: 229 PVMHTFRGKDLASYDHPHWIGGVGL-IGGAPGTDALHAAEVMLMLGSDYPYS-----EFL 282 Query: 95 TKDRTFVHVDIEPTQIGRVFGPDYGIVSDAKAALKLFIEVAKELKAAGNL-----PDRSA 149 + +D +GR GI A++ +E E K G R Sbjct: 283 PTKSKVIQIDERGFVLGRRAPVTLGITGSVAPAVQQLLERV-EGKTDGAFLQQANAHRER 341 Query: 150 WLA--DCQERKRTLHRRTHFEEVPIKPQRVYEEMNRAFGRDTCYVSTIGLSQIAAAQFLH 207 W A D + H++ IKPQ V ++ D +V G+ + ++ Sbjct: 342 WNAKLDSAAEIKRHHKK-------IKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIR 394 Query: 208 VYKDRHWINCGQAGPLGWTISAALGVCAADPQRKVVAISGDYDFQFMIEELAVGAQFNLP 267 + + +G ++ A G+ A D +R+VV GD F ++ E + LP Sbjct: 395 QRGGQRILASFNNAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLP 454 Query: 268 YLHVVVNNSYLGLIRQAQRGFEMDYCVQLAFENINAPELNGYGVDHVAVVEGLGCKAIRV 327 VV NN GL+ EM+ AFE P D A G + V Sbjct: 455 VKVVVFNNREWGLVH-----LEMEEAGMPAFEGSEFPN-----PDFAAFARACGGQGFSV 504 Query: 328 TNPNEIQDAF---------AVAQRWMAEYRVPVVVEIILERV 360 P E++ AV ++ +PV+ I +E++ Sbjct: 505 RTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPHIKMEQI 546 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,277,272 Number of extensions: 54235 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 3 Length of query: 393 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 306 Effective length of database: 1,207,925 Effective search space: 369625050 Effective search space used: 369625050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)