BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0025.0082.p
         (323 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312558|ref|YP_003776650.1| transmembrane protein [Herbaspi...   494   e-141

>gi|300312558|ref|YP_003776650.1| transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 432

 Score =  494 bits (1272), Expect = e-141,   Method: Compositional matrix adjust.
 Identities = 242/287 (84%), Positives = 272/287 (94%)

Query: 1   MTFLDKFFKLSDNGTTVRTELLAGLTTFLTMAYIIFVNPSILGDAGMPKDSVFVATCVAA 60
           M  L+KFF+L +NGTT+RTELLAGLTTFLTMAYIIFVNPSILGDAGMPKD+VFVATC+AA
Sbjct: 1   MNALNKFFRLDENGTTIRTELLAGLTTFLTMAYIIFVNPSILGDAGMPKDAVFVATCIAA 60

Query: 61  AIGTLIMGLYANYPIGLAPGMGLNAYFAYAVVKGMGFPWQAALGAVFISGCLFLLVSLFR 120
           A+GTLIMGLYANYPIGLAPGMGLNAYFAYAVVKGMGFPWQAALGAV ISGCLFLLVS+ R
Sbjct: 61  AVGTLIMGLYANYPIGLAPGMGLNAYFAYAVVKGMGFPWQAALGAVLISGCLFLLVSVLR 120

Query: 121 IRELIINAIPHSLRTAIPAGIGLFLALISLKNAGIVAASPATFVTMGDLHQAAPVLAIIG 180
           +RELII++IP SLRTAIPAGIGLFL +ISLK+AGI+AA+PATFVT+GDLHQ   V+AIIG
Sbjct: 121 VRELIISSIPRSLRTAIPAGIGLFLGIISLKSAGIIAANPATFVTLGDLHQPPAVMAIIG 180

Query: 181 FLVIVALDQLKIRGALLIGILTVTVLSFIFGGNHFNGVFSMPPSISPTLFQLDLKGAISM 240
           FLVIVALD+LK+RGALLIGIL VTVLSF+FGGNHF+G+F+ PPSI+PTL QLD+KGA+SM
Sbjct: 181 FLVIVALDRLKVRGALLIGILLVTVLSFVFGGNHFSGIFAAPPSIAPTLLQLDIKGALSM 240

Query: 241 GLLNVVLVFFLVELFDATGTLMGVAQRAGLVKNGKIERINKALLADS 287
           GLLNVVLVFFLVELFDATGTLMGVA RAGL+K GK+ER+NKAL+ADS
Sbjct: 241 GLLNVVLVFFLVELFDATGTLMGVATRAGLMKEGKMERLNKALMADS 287


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.328    0.143    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 981,078
Number of extensions: 42406
Number of successful extensions: 184
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 183
Number of HSP's successfully gapped: 1
Length of query: 323
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 238
Effective length of database: 1,217,395
Effective search space: 289740010
Effective search space used: 289740010
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 131 (55.1 bits)