BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0025.0060.p
         (300 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum serop...   439   e-124
gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil...    90   2e-19

>gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum
           seropedicae SmR1]
          Length = 300

 Score =  439 bits (1128), Expect = e-124,   Method: Compositional matrix adjust.
 Identities = 201/289 (69%), Positives = 242/289 (83%)

Query: 4   SRSEYLDIRGLQYHVRHWGDPQAPKLFMVHGWMDISASFQFMVDCLQQEWHVIAPDWRGF 63
           SRSE+L IRGL+YHVRHWGDP+APK+FM+HGWMD+SASFQFMVD LQ +WHVIAPDWRGF
Sbjct: 10  SRSEFLTIRGLRYHVRHWGDPEAPKIFMLHGWMDMSASFQFMVDALQGQWHVIAPDWRGF 69

Query: 64  GLTERPQADTYWYPDYLADLDALLLHYSPDEAVNLLGHSLGGNVVGLYAGVRPQRIAKLV 123
           GLTER QAD+YW+PDYLADLDA++ HY+  EA+NLLGHS+GGNVV LYAGVRPQRI +L+
Sbjct: 70  GLTERSQADSYWFPDYLADLDAIIDHYAGSEAINLLGHSMGGNVVTLYAGVRPQRIKRLI 129

Query: 124 NLEGFGLPGTKPEDAPGRFAKWLDELLEKPEMRSYPSLDGVIARLQKTNPRLSRERAVFL 183
           NLEG GLP T+PE APGRFA+W+DE+  +P +R Y +L+ V  RLQ+ N RLS ERA FL
Sbjct: 130 NLEGLGLPATQPEQAPGRFAQWMDEVRNRPVLRGYATLEEVAQRLQRNNARLSDERAAFL 189

Query: 184 AAHWAGQNEEGNWEILGDPAHKNTNPLLYRIDEVTACWAKISAPVLWVEAAETDIWRWFG 243
           A HWA Q++ G W+ILGDPAHK  +P+LYR DE+ ACW +I+APVLW+EA ++DIWR+FG
Sbjct: 190 AQHWAQQDDSGLWQILGDPAHKQVSPILYRADEMMACWRRITAPVLWIEAIDSDIWRFFG 249

Query: 244 DNALMRAEIDRRIAFIPDVRTEIIPDAGHMLHHDQPELLAAAIERFLAE 292
             +L R EIDRRIA IP V+ E+I DAGHMLHHDQPE LA  IE FL +
Sbjct: 250 PASLKREEIDRRIACIPRVQVEMIADAGHMLHHDQPEQLAQLIEAFLQQ 298


>gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily
           protein [Herbaspirillum seropedicae SmR1]
          Length = 289

 Score = 90.1 bits (222), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 82/299 (27%), Positives = 134/299 (44%), Gaps = 18/299 (6%)

Query: 5   RSEYLDIRGLQYHVRHWGDPQAPKLFMVHGWMDISASFQFMVDCLQQEWHVIAPDWRGFG 64
           R   L +  L +H+  WGDPQ   L ++HG      +F  +   L   +  IA D RG G
Sbjct: 4   RDYLLTLSALNFHITEWGDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQRGRG 63

Query: 65  LTERPQADTYWYPDYLADLDALLLHYSPDEAVNLLGHSLGGNVVGLYAGVRPQRIAKLVN 124
            ++      Y+   Y+AD++AL+   +  +  +LLGHS+GG    +YA   P ++ +L+ 
Sbjct: 64  QSDWDAGANYYTDAYVADVEALVARLNLRQ-FDLLGHSMGGINAIVYAARHPAKVRRLI- 121

Query: 125 LEGFGLPGTKPEDAPGRFAKWLDELLEKPEMRSYPSLDGVIARLQKTNPRLSRERAVFLA 184
           +E  G PG   E++PG   +   EL   P    + S D     ++   P ++ E      
Sbjct: 122 IEDAG-PGAF-ENSPG-ATRIRRELATTPS--HFASWDEASTFMRALRPSVTEEARQQRL 176

Query: 185 AHWAGQNEEGNWEILGDP---AHKNTNPLLYRIDEVTACWAKISAPVLWVEAAETDIWRW 241
            +      +G +    D    +    +P L R+ ++      +  P L V    +D    
Sbjct: 177 HNMLKPAAQGGYTWRYDHVGISSTRLSPDLSRVVDLRPHIMALMCPTLVVRGERSDYL-- 234

Query: 242 FGDNALMRAEIDRRIAFIPDVRTEIIPDAGHMLHHDQPELLAAAIERFLAESAAKQVSK 300
              +A M AE+ +  A I    T  I DAGH +H DQPE  A  + +FLA+   + V +
Sbjct: 235 ---SAEMAAEMRQLNARI---LTSTIADAGHYIHDDQPEAFAEVVRQFLAQEQHETVEE 287


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.138    0.447 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,058,967
Number of extensions: 46878
Number of successful extensions: 157
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 160
Number of HSP's successfully gapped: 2
Length of query: 300
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 215
Effective length of database: 1,217,395
Effective search space: 261739925
Effective search space used: 261739925
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 130 (54.7 bits)