BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0058.p (617 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 714 0.0 gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 189 7e-49 gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 81 3e-16 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 714 bits (1844), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/381 (87%), Positives = 359/381 (94%) Query: 1 MNDKKNYGFTTTILHSDRQKDIEHGSLHKPIHTSVAFGYKDARQLAEVFQGKQSGYRYGR 60 MNDKK YGFTTTILHSDRQK IEHGSLHKPIHTSV FGY+DARQLAEVFQGKQ GYRYGR Sbjct: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 Query: 61 QGNPTVSALEDKVTRMEDGLSTICFATGMAAIGAVIQGLLREGDHVVSSAFLFGNTNSMW 120 QGNPTV+ALEDK+T+MEDG STICFATGMAAIGA++QGLLREGDHVVSSAFLFGNTNS+W Sbjct: 61 QGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 Query: 121 MTVHAQGARVSMVDATDVKLVEAALTPQTRIVFVETIANPRTQIADLKRIGELCRERGIL 180 MTV AQGA+VSMVDATDVK VEAA+T TR+VFVETIANPRTQ+ADLKRIGELCRERGIL Sbjct: 121 MTVGAQGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGIL 180 Query: 181 YIVDNTMTSPYLFQPKAVNAGLVVNSLTKSIGGHGNALGGALTDTGLYDWSRYPHIADNY 240 Y+VDNTMTSPYLF+PK V AGLVVNSLTKSIGGHGNALGGALTDTG +DW+RYPHIA+NY Sbjct: 181 YVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAENY 240 Query: 241 KKNPQPQWGMAQIRAKALRDFGASLGPEAAHHIAVGAETIALRQDRECANALAVAQMLDA 300 KKNP PQWGMAQIRAKALRDFG SLGPEAAHHIAVGAETIALRQ+REC NALA+AQML A Sbjct: 241 KKNPAPQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQMLQA 300 Query: 301 DPRVAAVYYPGLPSHPQHALATELFRSYGSLLSFELKDGIDCFDYLNRLRLAVASSNLGD 360 D RVAAVYYPGL SHPQHAL+ LFRS+GSL+SFELKDGIDCFDYLNRLRLA+ +SNLGD Sbjct: 301 DERVAAVYYPGLESHPQHALSKALFRSFGSLMSFELKDGIDCFDYLNRLRLAIPTSNLGD 360 Query: 361 TRTLVIPVAHTIFFEMGQERR 381 TRTLVIPVAHTIF+EMG ERR Sbjct: 361 TRTLVIPVAHTIFYEMGAERR 381 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 189 bits (479), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 128/389 (32%), Positives = 201/389 (51%), Gaps = 21/389 (5%) Query: 1 MNDKKNYGFTTTILHSDRQKDIEHGSLHKPIHTSVAFGYKDARQLAEVFQGKQSGYRYGR 60 M D + F T +H D ++ PI+ +VAF + D + A++F K G Y R Sbjct: 1 MPDNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 60 Query: 61 QGNPTVSALEDKVTRMEDGLSTICFATGMAAIGAVIQGLLREGDHVVSSAFLFGNTNSMW 120 NPT LE ++ +E G+ + A+G AA+ IQ + GD++VS++ L+G T +++ Sbjct: 61 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 120 Query: 121 M-TVHAQGARVSMVDATDVKLVEAALTPQTRIVFVETIANPRTQIADLKRIGELCRERGI 179 T+ G + D E + +T+ +++E+I NP I D+ + ++ G+ Sbjct: 121 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV 180 Query: 180 LYIVDNTMTSPYLFQPKAVNAGLVVNSLTKSIGGHGNALGGALTDTGLYDWSRYPHIADN 239 IVDNT+ +PYL +P A +VV+SLTK +GGHG LGGA+ D+G + W+++ Sbjct: 181 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQ---R 237 Query: 240 YKKNPQPQWG---------------MAQIRAKALRDFGASLGPEAAHHIAVGAETIALRQ 284 +K+ +P + + R LR+ GA+L P A I G ET+ LR Sbjct: 238 FKRLNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRL 297 Query: 285 DRECANALAVAQMLDADPRVAAVYYPGLPSHPQHALATELFRSYGS-LLSFELKDGID-C 342 DR AN LAVAQ L P+V V Y GL HP HALA F+ S +L+F + +G + Sbjct: 298 DRITANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANGREGG 357 Query: 343 FDYLNRLRLAVASSNLGDTRTLVIPVAHT 371 + + L+L N+GD ++L A T Sbjct: 358 ARFQDALQLFTRLVNIGDAKSLATHPAST 386 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 80.9 bits (198), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 90/404 (22%), Positives = 157/404 (38%), Gaps = 49/404 (12%) Query: 1 MNDKKNYGFT--TTILHSDRQKDIEHGSLHKPIHTSVAFGYKDARQLAEVFQGKQSGYRY 58 M+D K T T+++HS + + IH + + D + ++ GY Y Sbjct: 1 MSDPKQTSLTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTY 60 Query: 59 GRQGNPTVSALEDKVTRMEDGLSTICFATGMAAIGAVIQGLLREGDHVVSSAFLFGNTNS 118 G G PT LE ++ +E G +G+AAI V L+ GD V+ ++G + Sbjct: 61 GLHGTPTSFVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKD 120 Query: 119 M--WMTVHAQGARVSMVDATDVKLVEAALTPQTRIVFVETIANPRTQIADLKRIGELCRE 176 + W+ Q D + + + P TR+V+ E + ++ D+ + Sbjct: 121 LGQWLERDFQ-ITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHA 179 Query: 177 RGILYIVDNTMTSPYLFQPKAVNAGLVVNSLTKSIGGHGNALGGALTDTGLYDWSRYPHI 236 +G+L +DNT ++ F+ +V+ ++TK G + L GA+ I Sbjct: 180 KGVLVAIDNTWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAV-------------I 226 Query: 237 ADNYKKNPQPQWGMAQIRAKALRDFGASLGPEAAHHIAVGAETIALRQDRECANALAVAQ 296 + N + + +A +R G +G + + + ++ LR + A A VA Sbjct: 227 VRDQALNEKLE--LAHMR------LGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAA 278 Query: 297 MLDADPRVAAVYYPGLPSHPQHALATELFRSYGSLLSFELKDGIDCFD----------YL 346 L P + AV +P P HA F G L S FD ++ Sbjct: 279 WLKQRPEIVAVLHPAFEDCPGHAHWKRDFSGAGGLFSV-------IFDPRYVEAQTDRFV 331 Query: 347 NRLRLAVASSNLGDTRTLVIPVAHTIFFEMGQERRQQFVRGLVV 390 + LRL + G +L +P + M RR G +V Sbjct: 332 DSLRLFKIGYSWGGAHSLCVP------YRMKGMRRAWMREGQLV 369 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,951,887 Number of extensions: 82737 Number of successful extensions: 279 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 278 Number of HSP's successfully gapped: 3 Length of query: 617 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 526 Effective length of database: 1,188,985 Effective search space: 625406110 Effective search space used: 625406110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 133 (55.8 bits)