BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0057.p (67 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312583|ref|YP_003776675.1| glutamine phosphoribosylpyropho... 123 3e-30 >gi|300312583|ref|YP_003776675.1| glutamine phosphoribosylpyrophosphate amidotransferase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 123 bits (308), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 57/67 (85%), Positives = 64/67 (95%) Query: 1 VEDLKRSISDINPALRNFEASCFDGNYITGDISRDYLDRIEFARKNPKPALEDAVRSQLN 60 VE LKR+ISD NPAL+ FEASCFDG+YITGDI+++YLDRIE+ARKNPKPALEDAVRSQLN Sbjct: 440 VEALKRAISDANPALKQFEASCFDGHYITGDITQEYLDRIEYARKNPKPALEDAVRSQLN 499 Query: 61 LNLARVD 67 LNLARVD Sbjct: 500 LNLARVD 506 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 189,005 Number of extensions: 5796 Number of successful extensions: 16 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 16 Number of HSP's successfully gapped: 1 Length of query: 67 Length of database: 1,619,870 Length adjustment: 39 Effective length of query: 28 Effective length of database: 1,435,205 Effective search space: 40185740 Effective search space used: 40185740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)