BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0042.p (226 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312595|ref|YP_003776687.1| 3-isopropylmalate dehydratase s... 410 e-116 >gi|300312595|ref|YP_003776687.1| 3-isopropylmalate dehydratase small subunit [Herbaspirillum seropedicae SmR1] Length = 215 Score = 410 bits (1054), Expect = e-116, Method: Compositional matrix adjust. Identities = 187/215 (86%), Positives = 207/215 (96%) Query: 12 MNKFIVHDGLVAPLDRANVDTDAIIPKQFLKSIQRSGFGPNLFDEWRYLDHGEPGMDNSK 71 MNKFIVH+GLVAPLDRANVDTDAIIPKQFLKSI+RSGFGPNLFDEWRYLDHGEPGMDNSK Sbjct: 1 MNKFIVHEGLVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDHGEPGMDNSK 60 Query: 72 RPLNPDFVLNQARYQGASVLLARKNFGCGSSREHAPWALDQYGFRAVIAPSFADIFFNNC 131 RPLNPDFVLNQ RYQG+S+LL+RKNFGCGSSREHAPWALDQ+GFRAVIAPSFADIFFNNC Sbjct: 61 RPLNPDFVLNQERYQGSSILLSRKNFGCGSSREHAPWALDQFGFRAVIAPSFADIFFNNC 120 Query: 132 FKNGLLPIVLPEQQIDRLFDEVKAFPGFRLIIDLEKQVITTANGSQTYPFEVGEFRKYCL 191 +KNGLLPIVL EQQ+D LF+EVKAFPG+RL+IDLEKQ+++T NGSQTYPFE+ EFRKYCL Sbjct: 121 YKNGLLPIVLTEQQVDHLFNEVKAFPGYRLVIDLEKQIVSTTNGSQTYPFEISEFRKYCL 180 Query: 192 LNGLDDIGLTLRQADKIRAFEERHLFDQPWLANTI 226 LNG DDIGLTLR+AD IRA+EE+HL +QPWL+NTI Sbjct: 181 LNGFDDIGLTLRKADTIRAYEEKHLHEQPWLSNTI 215 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.142 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 740,009 Number of extensions: 31362 Number of successful extensions: 62 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 62 Number of HSP's successfully gapped: 1 Length of query: 226 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 144 Effective length of database: 1,231,600 Effective search space: 177350400 Effective search space used: 177350400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 129 (54.3 bits)